The effect of inoculum preparation on the fully aerated growth of Saccharomyces cerevisiae with a glucose substrate.

Orlowski, Joanna H.; Barford, J. P.

doi:10.2323/jgam.33.113

Cited by 11 publications

(6 citation statements)

References 11 publications

(14 reference statements)

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“…The first alternative seems unlikely since the specific sugar uptake rate for the adapted cells growing on sucrose and glucose mixtures are almost as high as when the cells are growing on sucrose alone (Table 1); it is also higher than that of cells growing on glucose and fructose mixture (17). The biomass yields ranged from 0.13 to 0.16 g-biomass g-sugar-' which correspond to those earlier reported in chemostat cultures at high dilution rates (2,18) and in batch cultures at high sugar concentrations (6,16). The ethanol yields, oxygen uptake rates, carbon dioxide production rate and respiratory quotient (RQ) show that the growth was both fermentative and respiratory.…”

Section: And Discussionsupporting

confidence: 70%

“…When the cells are growing on sucrose and glucose mixtures, the specific growthh rate is on average 0.45 h-' for unadapted cells. This is the specific growth rate when the cells are growing on glucose alone (16). However, when the adapted cells were growing on mixtures containing 12 g 1-' glucose at inoculation, there was a slight but significant increase in the specific growth rate from 0.45 to 0.47 h -' and the specific sugar uptake rate from 18.8 to 20.1 mmol glucose equivalent g-biomass-' h-' (Table 1).…”

Section: And Discussionmentioning

confidence: 99%

“…The major metabolic parameters included specific growth rate (L.e ), biomass yield (YXS), ethanol yield (Ye), specific sugar uptake rate (QS) and specific ethanol production rate (Qe) and were calculated as previously described (16).…”

Section: Methodsmentioning

confidence: 99%

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Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Mwesigye¹,

Barford²

1996

J. Gen. Appl. Microbiol.

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Saccharomyces cerevisiae was grown on different concentrations of sucrose and glucose mixtures after adapting on sucrose. The yeast cells were found to have two different mechanisms by which sucrose was utilised: hydrolysis outside the cell membrane and direct transport into the cells. The mechanism by which sucrose was utilised depended on the initial concentration of glucose in the mixture and the adaptation state of the cells. In both cases, glucose was utilised first and invertase secretion was repressed when the glucose concentration was higher than 2 g l-'. The major important finding in our results is that, for fully sucrose-adapted cells, even in the presence of a repressive glucose concentration, the yeast cells were able to utilise sucrose, and most importantly, without first being hydrolysed to its constituent monosaccharides. Previously, it has been thought that direct transport of sucrose contributes only a small part of the process of utilisation of sucrose, but as our results indicate it can be the major mechanism of sucrose utilisation in S. cerevisiae if the cells are adapted on sucrose for long periods, glucose concentrations in the medium are high and/or invertase activities are limiting.The initial steps in the utilisation of sugars by yeast is either the passage of intact sugar molecules across the cell membrane or the hydrolysis outside the cell followed by entry of all or some of the hydrolytic products into the cell. Sugar transport into microbial cells across the plasmalemma can take place by active transport, facilitated diffusion and group translocation (19).Sucrose utilisation involves its hydrolysis outside the yeast cell by extracellular enzyme invertase ($-D-fructosidase) into glucose and fructose which are then transported into the cells and metabolised. Most Saccharomyces cerevisiae strains have two forms of invertase: an external large invertase, which is a glycoprotein, and an internal small invertase (9,15). Some strains of S. cerevisiae have been reported to transport sucrose directly into the cell (14,17,20).

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Section: And Discussionsupporting

confidence: 70%

Section: And Discussionmentioning

confidence: 99%

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Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Mwesigye¹,

Barford²

1996

J. Gen. Appl. Microbiol.

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“…Also batch culture is inherently unbalanced (Low et al, unpublished results) compared to steady-state chemostat cultures and is subject to significant variations in final cell and antibody yield. Inoculum preparation, volume and viability probably play a significant role in this regard, as with other eukaryotic systems (Orlowski and Barford, 1987).…”

Section: Discussionmentioning

confidence: 99%

A comparison of different culture methods for hybridoma propagation and monoclonal antibody production

et al. 1990

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A major variable to consider in the production of biologicals from mammalian cell cultures is the mode of operation, be it a batch, continuous, perfusion, fed-batch or other production method. The final choice must consider a number of fundamental and economic issues. Here we present some antibody production data from different cell lines using different modes of production and discuss the important factors for consideration in choosing a production strategy. It was found that the productivity of batch cultures was lower than that obtained in continuous and perfused cultures, but that productivity could be improved by implementing suitable feeding strategies. The antibody productivity of one cell line, MCL1, during exponential phase was not affected by media type or glucose level. The maximum productivity of two cell lines in continuous culture was found to occur at dilution rates below the maximum, from 0.019 to 0.030 hr-1.

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“…Although previous works have shown that Deinococcaceae have complex nutritional requirements [ 2 , 13 , 17 , 18 ], no study has been reported yet on the influence of the inoculum preparation on their growth properties (growth rate, maximal biomass concentration, glucose uptake). Inoculum preparation, including preservation method, revivification step and inoculum stages, is an essential element for the quality of the microbial culture and for bioprocess performances [ 19 ]. To achieve high performance culture, e.g., high biomass concentration and growth rate, a reproducible physiological state in the inoculum and a correct inoculation cell density are essential [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Characterization of the Growth of Deinococcus geothermalis DSM-11302: Effect of Inoculum Size, Growth Medium and Culture Conditions

et al. 2015

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Due to their remarkable resistance to extreme conditions, Deinococcaceae strains are of great interest to biotechnological prospects. However, the physiology of the extremophile strain Deinococcus geothermalis has scarcely been studied and is not well understood. The physiological behaviour was then studied in well-controlled conditions in flask and bioreactor cultures. The growth of D. geothermalis type strains was compared. Among the strains tested, the strain from the German Collection of Microorganisms (Deutsche Sammlung von Mikroorganismen DSM) DSM-11302 was found to give the highest biomass concentration and growth rate: in a complex medium with glucose, the growth rate reached 0.75 h−1 at 45 °C. Yeast extract concentration in the medium had significant constitutive and catalytic effects. Furthermore, the results showed that the physiological descriptors were not affected by the inoculum preparation steps. A batch culture of D. geothermalis DSM-11302 on defined medium was carried out: cells grew exponentially with a maximal growth rate of 0.28 h−1 and D. geothermalis DSM-11302 biomass reached 1.4 g·L−1 in 20 h. Then, 1.4 gDryCellWeight of biomass (X) was obtained from 5.6 g glucose (Glc) consumed as carbon source, corresponding to a yield of 0.3 CmolX·CmolGlc−1; cell specific oxygen uptake and carbon dioxide production rates reached 216 and 226 mmol.CmolX−1·h−1, respectively, and the respiratory quotient (QR) value varied from 1.1 to 1.7. This is the first time that kinetic parameters and yields are reported for D. geothermalis DSM-11302 grown on a mineral medium in well-controlled batch culture.

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The effect of inoculum preparation on the fully aerated growth of Saccharomyces cerevisiae with a glucose substrate.

Cited by 11 publications

References 11 publications

Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

A comparison of different culture methods for hybridoma propagation and monoclonal antibody production

Quantitative Characterization of the Growth of Deinococcus geothermalis DSM-11302: Effect of Inoculum Size, Growth Medium and Culture Conditions

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