The effect of inflammation on rat urinary bladder-dependent relaxation in coaxial bioassay system

İnci, Kubilay; Ismailoglu, Ugur B.; Şahin, Altan; Sungur, A.; Şahin-Erdemli, İnci

doi:10.1007/s00210-003-0710-y

Cited by 15 publications

(7 citation statements)

References 25 publications

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“…The effect was interpreted as being caused by an unidentified relaxant factor released from bladder tissue (Fovaeus et al, 1999). The presence of a relaxant factor, released from the rat bladder by acetylcholine, was confirmed by Inci et al (2003), who also showed that bladder inflammation did not alter the synthesis and/or release of this bladder-derived relaxant factor. Hawthorn et al (2000) reported the presence of a diffusable, urothelium-derived inhibitory factor in the pig bladder, which could not be identified, but did not appear to be NO, a COX product, a catecholamine, adenosine, GABA, or an endothelium-derived relaxing factor sensitive to apamin.…”

Section: Peripheral Targetsmentioning

confidence: 86%

Pharmacology of the Lower Urinary Tract: Basis for Current and Future Treatments of Urinary Incontinence

Andersson

Wein²

2004

Pharmacol Rev

452

364

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Section: Peripheral Targetsmentioning

confidence: 86%

Pharmacology of the Lower Urinary Tract: Basis for Current and Future Treatments of Urinary Incontinence

Andersson

Wein²

2004

Pharmacol Rev

452

364

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“…Gross histological changes manifested as severe inflammation and focal erosions of the bladder resulting from the urothelial cytotoxicity may therefore be associated with a corresponding loss in the bladder contractility (over 50%). CYCL-induced bladder cystitis, therefore, appears to be a result of reactive oxygen species (ROS) overload or NO induction in the system36,37 triggering a cascade of events beginning with the membrane damage.…”

Section: Discussionmentioning

confidence: 99%

Acacia Senegal Gum Exudate Offers Protection Against Cyclophosphamide‐Induced Urinary Bladder Cytotoxicity

Al-Yahya

Al-Majed²,

Gado³

et al. 2009

Oxidative Medicine and Cellular Longevity

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Cylophosphamide (CYCL) is a strong anticancer and immunosuppressive agent but its urotoxicity presents one of the major toxic effects that limit its wide usage particularly in high dose regimens. Therefore, this study aimed to investigate Acacia Senegal gum exudate, Gum Arabic (GA), for its possible role as a natural, nontoxic agent against CYCL-induced urotoxicity. Male Swiss albino rats were exposed to CYCL (150 mg/kg BW, once i.p) with or without GA oral supplementation (7.5 g/kg/day for 6 days) through drinking water. Glutathione (GSH), Malondialdehyde (MDA) and Nitric oxide (NO) bladder contents were assessed. Responsiveness of the bladder rings to acetylcholine (ACh) in vitro, microscopic and macroscopic features are also investigated. CYCL produced pronounced harmful effects on bladder urothelial lining with significant increases in (MDA) and NO levels in the tissue homogenates. Bladder-GSH content is dropped by over 60% following CYCL injection. Bladder contractility, as measured by its responsiveness to ACh, recorded a marked reduction. The isolated bladders exhibited such macroscopic changes as severe edema, inflammation and extravasation. The bladder weight increased as well. Histological changes were evident in the form of severe congestion, petechial hemorrhage and chronic inflammatory reaction in the lamina propria accompanied with desquamated epithelia. GA, a potential protective agent, produced an almost complete reversal of NO induction, lipid peroxidation or cellular GSH bladder contents in the GA + CYCL-treated group. Likewise, bladder inflammation and edema were reduced. Bladder rings showed a remarkable recovery in their responsiveness to ACh. Bladder histological examination showed a near normal configuration and structural integrity, with a significant reduction in inflammation and disappearance of focal erosions. These remarkable effects of GA may be attributed to its ability to neutralize acrolein, the reactive metabolite of CYCL and/or the resultant reactive oxygen metabolites, through a scavenging action. GA may limit the cascading events of CYCL-induced damage, initiating a cytoprotective effect leading to structural and functional recovery of the bladder tissues.

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“…Inci et al 31 have examined the effect of inflammation on rat urinary bladder-derived relaxant factor in the coaxial bioassay system. The authors concluded that CP-induced bladder inflammation did not alter the synthesis and/or release of the bladder-derived relaxing factor, which is neither a cyclo-oxygenase product nor NO, but the altered contractility may be due to inflammation-induced oedema.…”

Section: Stated Thatmentioning

confidence: 99%

Protective effect of taurine against cyclophosphamide‐induced urinary bladder toxicity in rats

Abd-Allah¹,

Gado

Al-Majed

et al. 2005

Clin Exp Pharma Physio

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1. In the present study, the effect of taurine, on cyclophosphamide (CP)-induced urinary bladder toxicity was investigated. 2. Administration of a single dose of CP (150 mg/kg, i.p.) induced cystitis, as manifested by marked congestion, oedema and extravasation in rat urinary bladder, as well as a marked desquamative damage to the urothium, severe inflammation in the lamina propria, focal erosions and polymorphonuclear leucocytes associated with occasional lymphocyte infiltration as determined by macroscopic and histopathological examination. 3. A significant decrease in the endogenous anti-oxidant compound glutathione and elevation of lipid peroxidation also resulted in rat urinary bladder tissue. 4. Cyclophosphamide-induced cystitis markedly affected the contractile function of the urinary bladder, as revealed by a significant inhibition of tissue responsiveness to acetylcholine (ACh) at different molar concentrations in vitro. 5. Conversely, pretreatment with taurine (1% in drinking water to reach a dose of 1 g/kg per day) for 7 days before and 1 day after CP injection produced a significant decrease in urinary bladder weight (oedema) and a marked decrease in vascular congestion and haemorrhage, as well as a profound improvement in histological structure. Moreover, taurine pretreatment resulted in a significant decrease in lipid peroxide in urinary bladder tissue and glutathione content was greatly restored. 6. Urinary bladder rings isolated from rats treated concurrently with taurine and CP showed a significant increase in their responsiveness to ACh compared with the CP group. 7. These results suggest that taurine offers a protective effect against CP-induced urinary bladder toxicity and may, therefore, decrease the limitation on its clinical application. These results merit extension and further investigation of the impact of taurine on CP antitumour activity.

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The effect of inflammation on rat urinary bladder-dependent relaxation in coaxial bioassay system

Cited by 15 publications

References 25 publications

Pharmacology of the Lower Urinary Tract: Basis for Current and Future Treatments of Urinary Incontinence

Pharmacology of the Lower Urinary Tract: Basis for Current and Future Treatments of Urinary Incontinence

Acacia Senegal Gum Exudate Offers Protection Against Cyclophosphamide‐Induced Urinary Bladder Cytotoxicity

Protective effect of taurine against cyclophosphamide‐induced urinary bladder toxicity in rats

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