The effect of indole-3-carbinol on PIN1 and PIN2 in Arabidopsis roots

Katz, Ella; Nisani, Sophia; Sela, Mor; Behar, Hila; Chamovitz, Daniel

doi:10.1080/15592324.2015.1062200

Cited by 19 publications

(18 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kerwin et al, 2015;; D J Kliebenstein, Kroymann, et al, 2001;D J Kliebenstein, Lambrix, Reichelt, Gershenzon, & Mitchell-Olds, 2001; James E. Rodman, Kruckeberg, & Al-Shehbaz, 1981;James Eric Rodman, 1980;Sønderby et al, 2010;Wright, Lauga, & Charlesworth, 2002). GSLs consist of a common core structure with a highly diverse side chain that determines the GSLs biological activity in defense, growth, development and abiotic stress resistance (Beekwilder et al, 2008;Hansen et al, 2008;Hasegawa, Yamada, Kosemura, Yamamura, & Hasegawa, 2000;Katz et al, 2020;Katz, Nisani, Sela, Behar, & Chamovitz, 2015;Malinovsky et al, 2017;Salehin et al, 2019;Yamada et al, 2003). The Arabidopsis-GSL system is an optimal model to study the species wide processes driving specialized metabolite variation because the identity of the whole biosynthetic pathway is known, including the major causal loci for natural variation (Benderoth et al, 2006;Brachi et al, 2015;Chan et al, 2011Chan et al, , 2010D.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe

Katz

Bagaza

Holden

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Plants face a variety of challenges within their ever-changing environment. Diverse metabolites are central to the plants ability to overcome these challenges. Understanding the environmental and genetic factors influencing the variation in specialized metabolites is the key to understand how plants survive and develop under changing environments. Here we measure the variation in specialized metabolites across a population of 797 natural Arabidopsis thaliana accessions. We show a combination of geography, environmental parameters, demography, and different genetic processes that creates a specific pattern in their accumulation and distribution. By identifying and tracking causal polymorphisms at multiple loci controlling metabolites variation we show that each locus displays extensive allelic heterogeneity with signatures of both parallel and convergent evolutionary processes. These loci combine epistatically and show differing relationships to environmental parameters leading to different distributions. This provides a detailed perspective about the complexity of the forces and mechanisms that shape the accumulation and distribution of a family of specialized metabolites critical for plant fitness.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe

Katz

Bagaza

Holden

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…While normally a number of CycB1-expressing cells are visible in the root meristem, following I3C treatment, no CycB1-containing cells were detected, indicating a cessation of cell division. This conclusion is supported by transcript-profiling results showing the downregulation of cell cycle genes six hours following exposure to I3C 55 .…”

Section: Indole-3-carbinol and Plantsmentioning

confidence: 60%

“…Specific autophagy targets damaged proteins and other cellular components for degradation 59 and is seen in the co-localization of the GFP-AtATG8A and mCherry-AtNBR1 marker proteins in autophagosomes following I3C treatment. The I3C-induced autophagy targets the TIR1 auxin receptor, thus connecting I3C-dependent inhibition of auxin signaling 55 and the I3C induction of autophagy 58 .…”

Section: Indole-3-carbinol and Plantsmentioning

confidence: 99%

Indole-3-carbinol: a plant hormone combatting cancer

2018

Self Cite

View full text Add to dashboard Cite

A diet rich in cruciferous vegetables such as cauliflower, broccoli, and cabbage has long been considered healthy, and various epidemiological studies suggest that the consumption of cruciferous vegetables contributes to a cancer-protecting diet. While these vegetables contain a vast array of phytochemicals, the mechanism by which these vegetables counteract cancer is still largely unresolved. Numerous in situ studies have implicated indole-3-carbinol, a breakdown product of the glucosinolate indole-3-ylmethylglucosinolate, as one of the phytochemicals with anti-cancer properties. Indole-3-carbinol influences a range of cellular processes, but the mechanisms by which it acts on cancer cells are slowly being revealed. Recent studies on the role of indole-3-carbinol in Arabidopsis opens the door for cross-kingdom comparisons that can help in understanding the roles of this important phytohormone in both plant biology and combatting cancer.

show abstract

“…The highly reactive ITC hydrolysis product gives rise to a multitude of different compounds ( Agerbirk et al, 2009 ). One of these, I3C, was recently reported to exhibit auxin-antagonistic behavior via its competitive binding to TIR1 – the major auxin receptor ( Katz et al, 2015a , b ). This proposes a function of an I3G hydrolysis product as inhibitor of auxin signaling upon attack.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Modeling of Indole Glucosinolate Hydrolysis and Its Impact on Auxin Signaling

et al. 2018

View full text Add to dashboard Cite

Plants release chemicals to deter attackers. Arabidopsis thaliana relies on multiple defense compounds, including indol-3-ylmethyl glucosinolate (I3G), which upon hydrolysis initiated by myrosinase enzymes releases a multitude of bioactive compounds, among others, indole-3-acetonitrile and indole-3-acetoisothiocyanate. The highly unstable isothiocyanate rapidly reacts with other molecules. One of the products, indole-3-carbinol, was reported to inhibit auxin signaling through binding to the TIR1 auxin receptor. On the contrary, the nitrile product of I3G hydrolysis can be converted by nitrilase enzymes to form the primary auxin molecule, indole-3-acetic acid, which activates TIR1. This suggests that auxin signaling is subject to both antagonistic and protagonistic effects of I3G hydrolysis upon attack. We hypothesize that I3G hydrolysis and auxin signaling form an incoherent feedforward loop and we build a mathematical model to examine the regulatory network dynamics. We use molecular docking to investigate the possible antagonistic properties of different I3G hydrolysis products by competitive binding to the TIR1 receptor. Our simulations reveal an uncoupling of auxin concentration and signaling, and we determine that enzyme activity and antagonist binding affinity are key parameters for this uncoupling. The molecular docking predicts that several I3G hydrolysis products strongly antagonize auxin signaling. By comparing a tissue disrupting attack – e.g., by chewing insects or necrotrophic pathogens that causes rapid release of I3G hydrolysis products – to sustained cell-autonomous I3G hydrolysis, e.g., upon infection by biotrophic pathogens, we find that each scenario gives rise to distinct auxin signaling dynamics. This suggests that plants have different defense versus growth strategies depending on the nature of the attack.

show abstract

The effect of indole-3-carbinol on PIN1 and PIN2 in Arabidopsis roots

Cited by 19 publications

References 22 publications

Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe

Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe

Indole-3-carbinol: a plant hormone combatting cancer

Dynamic Modeling of Indole Glucosinolate Hydrolysis and Its Impact on Auxin Signaling

Contact Info

Product

Resources

About