The effect of IHF on σ S selectivity of the phoA and pst promoters of Escherichia coli

Taschner, Natália Pasternak; Yagil, Ezra; Spira, Beny

doi:10.1007/s00203-005-0082-4

Cited by 15 publications

(7 citation statements)

References 23 publications

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“…As expected, the cultures in low-Pi medium displayed highly induced AP activity, confirming that they have entered the Pi-starvation phase while those grown in high-Pi showed only basal AP levels. PpstS showed the strongest CAT activity and a Pi-dependent induction of *11-fold (Table 2, line A), confirming previous data (Taschner et al 2006). Both the PpstC and PpstB clones presented a weaker but constitutive CAT activity (Table 2, lines B and D) and PpstA (line C) showed a negligible activity, lower than the negative control (line F).…”

Section: Resultssupporting

confidence: 91%

“…The new plasmid constructs and pBS11 (pstS promoter cloned in pKK232-8) (Taschner et al 2006) were transformed into the wild-type strain MG1655 and into its isogenic phoB mutant (strain EY1491) to test if the cloned fragments display promoter activity and if they are phoBdependent. Each transformant was tested for growth on LB plates supplemented with chloramphenicol.…”

Section: Resultsmentioning

confidence: 99%

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Alternative promoters in the pst operon of Escherichia coli

et al. 2010

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The pst operon of Escherichia coli is composed of five genes pstS, pstC, pstA, pstB and phoU, that encode a high-affinity phosphate transport system and a negative regulator of the PHO regulon. Transcription of pst is induced under phosphate shortage and is initiated at the promoter located upstream of the first gene of the operon, pstS. Here, we show by four different technical approaches the existence of additional internal promoters upstream of pstC, pstB and phoU. These promoters are not induced by Pi-limitation and do not possess PHO-box sequences. Plasmids carrying the pst internal genes partially complement chromosomal mutations in their corresponding genes, indicating that they are translated into functional proteins.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Alternative promoters in the pst operon of Escherichia coli

et al. 2010

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“…In bacteria, metabolism changes that allow cells to survive occur during environmental stress conditions (Weber et al, 2005;Jain et al, 2006;Klauck et al, 2007). Many lines of evidence suggest that the Pho regulon and the stress response are interrelated (Spira et al, 1995;Spira & Yagil, 1998;Ruiz & Silhavy, 2003;Taschner et al, 2004Taschner et al, , 2006Schurdell et al, 2007). Figure 2 illustrates interconnections between the Pho regulon and some key factors involved in nutritional and other stress responses.…”

Section: Environmental Stresses Stringent Response and The Pho Regulonmentioning

confidence: 99%

The phosphate regulon and bacterial virulence: a regulatory network connecting phosphate homeostasis and pathogenesis

et al. 2008

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Bacterial pathogens regulate virulence factor gene expression coordinately in response to environmental stimuli, including nutrient starvation. The phosphate (Pho) regulon plays a key role in phosphate homeostasis. It is controlled by the PhoR/PhoB two-component regulatory system. PhoR is an integral membrane signaling histidine kinase that, through an interaction with the ABC-type phosphate-specific transport (Pst) system and a protein called PhoU, somehow senses environmental inorganic phosphate (P(i)) levels. Under conditions of P(i) limitation (or in the absence of a Pst component or PhoU), PhoR activates its partner response regulator PhoB by phosphorylation, which, in turn, up- or down-regulates target genes. Single-cell profiling of PhoB activation has shown recently that Pho regulon gene expression exhibits a stochastic, "all-or-none" behavior. Recent studies have also shown that the Pho regulon plays a role in the virulence of several bacteria. Here, we present a comprehensive overview of the role of the Pho regulon in bacterial virulence. The Pho regulon is clearly not a simple regulatory circuit for controlling phosphate homeostasis; it is part of a complex network important for both bacterial virulence and stress response.

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“…The Pho regulon and the stress response may be interrelated [95,96,97,98,99,100]. The presence of glucose or mutations in cya or crp leads to the induction of phoA gene in phoR mutatnt.…”

Section: Phosphate Regulationmentioning

confidence: 99%

Regulation Systems of Bacteria such as Escherichia coli in Response to Nutrient Limitation and Environmental Stresses

2013

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An overview was made to understand the regulation system of a bacterial cell such as Escherichia coli in response to nutrient limitation such as carbon, nitrogen, phosphate, sulfur, ion sources, and environmental stresses such as oxidative stress, acid shock, heat shock, and solvent stresses. It is quite important to understand how the cell detects environmental signals, integrate such information, and how the cell system is regulated. As for catabolite regulation, F1,6B P (FDP), PEP, and PYR play important roles in enzyme level regulation together with transcriptional regulation by such transcription factors as Cra, Fis, CsrA, and cAMP-Crp. αKG plays an important role in the coordinated control between carbon (C)- and nitrogen (N)-limitations, where αKG inhibits enzyme I (EI) of phosphotransferase system (PTS), thus regulating the glucose uptake rate in accordance with N level. As such, multiple regulation systems are co-ordinated for the cell synthesis and energy generation against nutrient limitations and environmental stresses. As for oxidative stress, the TCA cycle both generates and scavenges the reactive oxygen species (ROSs), where NADPH produced at ICDH and the oxidative pentose phosphate pathways play an important role in coping with oxidative stress. Solvent resistant mechanism was also considered for the stresses caused by biofuels and biochemicals production in the cell.

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The effect of IHF on σ S selectivity of the phoA and pst promoters of Escherichia coli

Cited by 15 publications

References 23 publications

Alternative promoters in the pst operon of Escherichia coli

Alternative promoters in the pst operon of Escherichia coli

The phosphate regulon and bacterial virulence: a regulatory network connecting phosphate homeostasis and pathogenesis

Regulation Systems of Bacteria such as Escherichia coli in Response to Nutrient Limitation and Environmental Stresses

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