The effect of ibuprofen on expression of Cox-1/2-related miRNAs in MKN- 45 -derived cancer stem-like cells

Akrami, Hassan; Karimi, Behnam; Salehi, Zohreh; Sisakhtnezhad, Sajjad

doi:10.4103/jrptps.jrptps_9_18

Cited by 2 publications

(4 citation statements)

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“…The higher reactivity on the oxygen atoms in relation to fcan be linked to IBF mechanism of action, which is still not clarified. 84,85 In fact, given the IBF pKa, its dissociated form (IBF COO -) is supposed to be present in both the investigated pHs, being dominant in basic solutions. 42 The pronounced electric dipole and charge separation noticed for this structure suggests a more effective interaction with water, explaining the efficient drug release in alkaline solutions with very effective burst release processes (Figure 4).…”

Section: Discussionmentioning

confidence: 98%

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Ibuprofen-loaded biocompatible latex membrane for drug release: Characterization and molecular modeling

Lima

Pegorin

Miranda

et al. 2021

Journal of Applied Biomaterials & Functional Materials

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The incorporation of drugs and bioactive compounds in the natural rubber latex (NRL) matrix has been an alternative for the development of transdermal release membranes. Ibuprofen (IBF) is known to be used to treat inflammatory diseases, but when administered orally, high concentrations can cause some adverse problems. In this work, the incorporation of IBF in the NRL membranes was evaluated by physical-chemical, in vitro permeation, hemocompatibility and molecular modeling assays. In addition, the in vitro release profile of IBF in acid and basic media was analyzed during 96 h. The IBF-NRL membrane exhibited the absence of intermolecular bonding that could hinder drug release and presented compatible mechanical properties for applications as a cutaneous adhesive (0.58 and 1.12 MPa to Young’s modulus and rupture tension, respectively). The IBF-NRL system did not present a significant hemolysis degree (1.67%) within 24 h. The release test indicated that in the first hours of the study, 48.5% IBF was released at basic pH and 22.5% at acidic pH, which is characteristic of a burst effect. Then, a stable release profile was observed until the end of the assay, with total IBF release of 60% in alkaline medium and 50% in acidic medium. The drug permeation results indicated that the IBF-NRL membranes can be used for the local skin treatment with permeation of 3.11% of IBF. Dynamic Molecular simulations indicated a pronounced electric dipole in the ionized form of IBF, which suggests a more effective interaction with water, explaining the efficient drug release in alkaline solutions. In general, the results demonstrate that the IBF-NRL membrane has great potential for a new adhesive that can be used for the treatment of inflammatory processes and injuries.

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Section: Discussionmentioning

confidence: 98%

“…The higher reactivity on the oxygen atoms in relation to f – can be linked to IBF mechanism of action, which is still not clarified. 84,85…”

Section: Discussionmentioning

confidence: 99%

Ibuprofen-loaded biocompatible latex membrane for drug release: Characterization and molecular modeling

Lima

Pegorin

Miranda

et al. 2021

Journal of Applied Biomaterials & Functional Materials

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“…It has been found that overexpression of Cox-2 in cancer cell lines promotes their ability to invade surrounding tissues as well as increases cell invasion in gastric cancer. Some miRNAs downregulated the expression of Cox-1 and Cox-2 genes and thereby inhibited cell invasion [ 62 ]. This study investigated the expression of miRNAs that target Cox-1/2 mRNAs and evaluated the effect of SP on the expression of the Cox-1/2 mRNAs in HepG2 cells.…”

Section: Discussionmentioning

confidence: 99%

“…This study investigated the expression of miRNAs that target Cox-1/2 mRNAs and evaluated the effect of SP on the expression of the Cox-1/2 mRNAs in HepG2 cells. In the current study, miRNA and mRNA expression was performed to find the correlation in the expression of miRNAs (miR-146a and miR-155) and Cox-1/2 mRNA [ 62 ]. The present study shows a significant reduction of Cox-2 , this is an indication that SP might be targeting Cox-2 as one of the many mechanisms to inhibit inflammation.…”

Section: Discussionmentioning

confidence: 99%

Spirulina platensis Mitigates the Inhibition of Selected miRNAs that Promote Inflammation in HAART-Treated HepG2 Cells

Sibiya

Ghazi

Mohan

et al. 2022

Plants

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The introduction of highly active antiretroviral therapy (HAART) in the treatment of HIV/AIDS has recently gained popularity. In addition, the significant role of microRNA expression in HIV pathogenesis cannot be overlooked; hence the need to explore the mechanisms of microRNA expression in the presence of HAART and Spirulina platensis (SP) in HepG2 cells. This study investigates the biochemical mechanisms of microRNA expression in HepG2 cells in the presence of HAART, SP, and the potential synergistic effect of HAART–SP. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine cell viability following SP treatment. The cellular redox status was assessed using the quantification of intracellular reactive oxygen species (ROS), lipid peroxidation, and a lactate dehydrogenase (LDH) assay. The fluorometric JC-1 assay was used to determine mitochondrial polarisation. The quantitative polymerase chain reaction (qPCR) was also employed for micro-RNA and gene expressions. The results show that MiR-146a (p < 0.0001) and miR-155 (p < 0.0001) levels increased in SP-treated cells. However, only miR-146a (p < 0.0001) in HAART–SP indicated an increase, while miR-155 (p < 0.0001) in HAART–SP treatment indicated a significant decreased expression. Further inflammation analysis revealed that Cox-1 mRNA expression was reduced in SP-treated cells (p = 0.4129). However, Cox-1 expression was significantly increased in HAART–SP-treated cells (p < 0.0001). The investigation revealed that HepG2 cells exposed to HAART–SP treatment showed a significant decrease in Cox-2 (p < 0.0001) expression. mRNA expression also decreased in SP-treated cells (p < 0.0001); therefore, SP potentially controls inflammation by regulating microRNA expressions. Moreover, the positive synergistic effect is indicated by normalised intracellular ROS levels (p < 0.0001) in the HAART–SP treatment. We hereby recommend further investigation on the synergistic roles of SP and HAART in the expression of microRNA with more focus on inflammatory and oxidative pathways.

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The effect of ibuprofen on expression of Cox-1/2-related miRNAs in MKN- 45 -derived cancer stem-like cells

Cited by 2 publications

References 1 publication

Ibuprofen-loaded biocompatible latex membrane for drug release: Characterization and molecular modeling

Ibuprofen-loaded biocompatible latex membrane for drug release: Characterization and molecular modeling

Spirulina platensis Mitigates the Inhibition of Selected miRNAs that Promote Inflammation in HAART-Treated HepG2 Cells

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