The Effect of Trigonella foenum-graceum L. (Fenugreek) Towards Collagen Type I Alpha 1 (COL1A1) and Collagen Type III Alpha 1 (COL3A1) on Postmenopausal Woman's Fibroblast

Fenugreek (Trigonella foenum-graceum) increases postmenopausal fibroblast-associated COL1A1 and COL3A1 production dominantly through its binding to estrogen receptor beta

“…The administration of 2 µg/ml fenugreek extract increased the secretion of COL1A1 and COL3A1 in HDF. This result was in accordance with previous experimental study (Yusharyahya et al, 2019). Fenugreek extract demonstrated a similar mechanism of action to another phytoestrogen such as soybeans in inhibiting skin-aging process (Jiang et al, 2013;Pilsakova et al, 2010).…”

“…Fenugreek seeds were obtained from Badan Penelitian Tanaman Obat, Tawangmangu, Solo, Indonesia through methanol extraction process by the Agency for the Assessment and Application of Technology (Badan Pengkajian dan Penerapan Teknologi). The dose of fenugreek extract referred to the previous study, i.e., the optimal dose of 2 µg/ml with completed media solvent (Yusharyahya et al, 2019).…”

“…Based on the previous study on the comparison of Collagen Type 1 Alpha 1 (COL1A1) and Collagen Type 3 Alpha 1 (COL3A1) secretion on postmenopausal women's fibroblasts (Human Dermal Fibroblast/HDF), the administration of 2 µg/ml of fenugreek extract significantly increased collagen secretion compared to control (no-treatment) and 5 nM estradiol (Yusharyahya et al, 2019). This study aimed to identify the mechanism of action, especially in fibroblast-associated COL1A1 and COL3A1 production.…”

Anti-aging effects of fenugreek cream on postmenopausal skin: A randomized controlled trial

“…Several studies on fenugreek have demonstrated its benefits toward skin elasticity, moisture, and melanin concentration, but subjects involved in the studies were young males and females (Gade et al, 2015;Naveed et al, 2010;Waqas et al, 2010). Our previous in vitro research was able to show the stimulation of COL1A1 and COL3A1 production (Yusharyahya et al, 2019b) through activation of estrogen receptor (ER) α and ER β, particularly ER β , and elucidate the optimum concentration of fenugreek required (Yusharyahya et al, 2020).…”