The Effect of <i>TRANSPARENT TESTA2</i> on Seed Fatty Acid Biosynthesis and Tolerance to Environmental Stresses during Young Seedling Establishment in Arabidopsis

Chen, Mingxun; Wang, Zhong; Zhu, Yu; Li, Zhilan; Hussain, N.; Xuan, Lijie; Guo, Wanli; Zhang, Guoping; Jiang, Lixi

doi:10.1104/pp.112.202945

Cited by 86 publications

(74 citation statements)

References 54 publications

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“…Seed FAs were extracted and analyzed as reported previously in detail (Poirier et al, 1999;Chen et al, 2012). Briefly, total FAs were converted to FA methyl esters in methanol solution containing 1 M HCl for 2 h at 80°C.…”

Section: Measurement Of Seed Fasmentioning

confidence: 99%

“…The negative feedback effect of TTG1 on LEC2 and ABI3 plays a role in mediating FUS3 expression in seeds, thereby regulating the rate of seed storage reserve accumulation (Chen et al, 2015). An R2R3-MYB protein, MYB123 (TT2), was found to be a key regulator of not only proanthocyanidin deposition but also oil accumulation in seeds (Nesi et al, 2001;Chen et al, 2012;Wang et al, 2014). Knockout of MYB118, which also is a member of the R2R3-MYB TFs, caused the derepression of maturation-related genes, thus stimulating storage processes in endosperm (Barthole et al, 2014).…”

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MYB89 Transcription Factor Represses Seed Oil Accumulation

Jin

Duan

et al. 2016

Plant Physiol.

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ORCID IDs: 0000-0002-0738-7899 (M.Z.); 0000-0002-2121-9517 (M.C.).In many higher plants, seed oil accumulation is precisely controlled by intricate multilevel regulatory networks, among which transcriptional regulation mainly influences oil biosynthesis. In Arabidopsis (Arabidopsis thaliana), the master positive transcription factors, WRINKLED1 (WRI1) and LEAFY COTYLEDON1-LIKE (L1L), are important for seed oil accumulation. We found that an R2R3-MYB transcription factor, MYB89, was expressed predominantly in developing seeds during maturation. Oil and major fatty acid biosynthesis in seeds was significantly promoted by myb89-1 mutation and MYB89 knockdown; thus, MYB89 was an important repressor during seed oil accumulation. RNA sequencing revealed remarkable up-regulation of numerous genes involved in seed oil accumulation in myb89 seeds at 12 d after pollination. Posttranslational activation of a MYB89-glucocorticoid receptor fusion protein and chromatin immunoprecipitation assays demonstrated that MYB89 inhibited seed oil accumulation by directly repressing WRI1 and five key genes and by indirectly suppressing L1L and 11 key genes involved in oil biosynthesis during seed maturation. These results help us to understand the novel function of MYB89 and provide new insights into the regulatory network of transcriptional factors controlling seed oil accumulation in Arabidopsis.

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Section: Measurement Of Seed Fasmentioning

confidence: 99%

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confidence: 99%

MYB89 Transcription Factor Represses Seed Oil Accumulation

Jin

Duan

et al. 2016

Plant Physiol.

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“…Measurement of fatty acids in seeds was subsequently performed using the gas chromatograph (GC-2014, Shimadzu) as previously described (Poirier et al, 1999;Chen et al, 2012a). Starch and protein in seeds were measured as described before (Chen et al, 2012b).…”

Section: Measurement Of Fatty Acid Starch and Proteinmentioning

confidence: 99%

TRANSPARENT TESTA GLABRA1 Regulates the Accumulation of Seed Storage Reserves in Arabidopsis

Chen

Zhang

et al. 2015

Plant Physiol.

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ORCID IDs: 0000-0002-2121-9517 (M.C.); 0000-0002-9778-8855 (H.Y.).Seed storage reserves mainly consist of starch, triacylglycerols, and storage proteins. They not only provide energy for seed germination and seedling establishment, but also supply essential dietary nutrients for human beings and animals. So far, the regulatory networks that govern the accumulation of seed storage reserves in plants are still largely unknown. Here, we show that TRANSPARENT TESTA GLABRA1 (TTG1), which encodes a WD40 repeat transcription factor involved in many aspects of plant development, plays an important role in mediating the accumulation of seed storage reserves in Arabidopsis (Arabidopsis thaliana). The dry weight of ttg1-1 embryos significantly increases compared with that of wild-type embryos, which is accompanied by an increase in the contents of starch, total protein, and fatty acids in ttg1-1 seeds. FUSCA3 (FUS3), a master regulator of seed maturation, binds directly to the TTG1 genomic region and suppresses TTG1 expression in developing seeds. TTG1 negatively regulates the accumulation of seed storage proteins partially through transcriptional repression of 2S3, a gene encoding a 2S albumin precursor. TTG1 also indirectly suppresses the expression of genes involved in either seed development or synthesis/modification of fatty acids in developing seeds. In addition, we demonstrate that the maternal allele of the TTG1 gene suppresses the accumulation of storage proteins and fatty acids in seeds. Our results suggest that TTG1 is a direct target of FUS3 in the framework of the regulatory hierarchy controlling seed filling and regulates the accumulation of seed storage proteins and fatty acids during the seed maturation process.

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“…In plants, flavonoid derivatives determine the pigmentation pattern of vegetative organs and seeds (Nesi et al, 2001; Lepiniec et al, 2006) and are involved in multiple functions. For example, they play important roles in protecting plants against a wide range of environmental injuries Agati and Tattini, 2010;Agati et al, 2012;Chen et al, 2012b). Seed coat mutants include two major groups.…”

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TRANSPARENT TESTA8 Inhibits Seed Fatty Acid Accumulation by Targeting Several Seed Development Regulators in Arabidopsis

et al. 2014

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Fatty acids (FAs) and FA-derived complex lipids play important roles in plant growth and vegetative development and are a class of prominent metabolites stored in mature seeds. The factors and regulatory networks that control FA accumulation in plant seeds remain largely unknown. The role of TRANSPARENT TESTA8 (TT8) in the regulation of flavonoid biosynthesis and the formation of seed coat color is extensively studied; however, its function in affecting seed FA biosynthesis is poorly understood. In this article, we show that Arabidopsis (Arabidopsis thaliana) TT8 acts maternally to affect seed FA biosynthesis and inhibits seed FA accumulation by down-regulating a group of genes either critical to embryonic development or important in the FA biosynthesis pathway. Moreover, the tt8 mutation resulted in reduced deposition of protein in seeds during maturation. Posttranslational activation of a TT8-GLUCOCORTICOID RECEPTOR fusion protein and chromatin immunoprecipitation assays demonstrated that TT8 represses the activities of LEAFY COTYLEDON1, LEAFY COTYLEDON2, and FUSCA3, the critical transcriptional factors important for seed development, as well as CYTIDINEDIPHOSPHATE DIACYLGLYCEROL SYNTHASE2, which mediates glycerolipid biosynthesis. These results help us to understand the entire function of TT8 and increase our knowledge of the complicated networks regulating the formation of FA-derived complex lipids in plant seeds.

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The Effect of TRANSPARENT TESTA2 on Seed Fatty Acid Biosynthesis and Tolerance to Environmental Stresses during Young Seedling Establishment in Arabidopsis

Cited by 86 publications

References 54 publications

MYB89 Transcription Factor Represses Seed Oil Accumulation

MYB89 Transcription Factor Represses Seed Oil Accumulation

TRANSPARENT TESTA GLABRA1 Regulates the Accumulation of Seed Storage Reserves in Arabidopsis

TRANSPARENT TESTA8 Inhibits Seed Fatty Acid Accumulation by Targeting Several Seed Development Regulators in Arabidopsis

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