The effect ofPanax notoginsengsaponins on oxidative stress induced by PCV2 infection in immune cells:in vitroandin vivostudies

Wang, Qiu Hua; Kuang, Na; Hu, Wen; Yin, Dan; Wei, Ying; Hu, Ting

doi:10.4142/jvs.2020.21.e61

Cited by 16 publications

(9 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…17 Treatment of porcine circovirus 2-infected porcine lung cells with PNS resulted in a significant reduction in intracellular NO content and xanthine oxidase activity, which suggested that PNS could regulate the oxidative stress of immune cells. 18 PNS decreased serum MDA, lactate dehydrogenase, myocardial tissue superoxide dismutase, and reactive oxygen species. 19 Ginsenoside Rg1, a natural compound isolated from PNS, promoted HUVEC progression into the S and G2/M phases of the cell cycle and markedly elevated EGFR secretion and had a notable effect on HUVEC migration and tube formation.…”

Section: Discussionmentioning

confidence: 88%

“…Dammarane‐type triterpenoid saponins could inhibit NO activities in a dose‐dependent manner 17 . Treatment of porcine circovirus 2‐infected porcine lung cells with PNS resulted in a significant reduction in intracellular NO content and xanthine oxidase activity, which suggested that PNS could regulate the oxidative stress of immune cells 18 . PNS decreased serum MDA, lactate dehydrogenase, myocardial tissue superoxide dismutase, and reactive oxygen species 19 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Panax notoginseng saponins improves healing of high glucose‐induced wound through the GSK‐3β/β‐catenin pathway

Lei

Gao

Duan

et al. 2022

Environmental Toxicology

View full text Add to dashboard Cite

Chronic non-healing wounds are one of the most common complications of diabetes mellitus and results in a huge physical and mental burden for patients. Panax notoginseng saponins (PNS) have a wide range of applications in anti-apoptosis, anti-oxidation, and promoting blood circulation. Our study aimed to explore whether PNS could improve diabetic wound healing. High-glucose (HG, 30 Mm) were used to incubated human umbilical vein endothelial cells (HUVECs) to simulate the hyperglycemia environment in vivo, and 200 μg/ml (optimum harmless concentration screened) PNS was added into HG-incubated HUVECs to investigate the protective effect of PNS on the cells. Compared with control, high glucose treatment significantly suppressed HUVEC proliferation, invasion, migration, angiogenesis, malondialdehyde (MDA) production and nitric oxide (NO) release, promoted cell apoptosis, and deactivated the GSK-3β/β-catenin/ VEGF pathway. PNS treatment could largely rescue the effects of HG on cell dysfunction and improve the deactivation of GSK-3β/β-catenin/VEGF pathway. ICG-001, a small molecular β-catenin inhibitor that can selectively antagonize β-catenin mediated transcriptional activity, could eliminate the protective effects of PNS on cell dysfunction and activation of GSK-3β/β-catenin/VEGF pathway. Moreover, Furthermore, a diabetic model (50 mg/kg streptozotocin induced) with back skin wound was established in rats, and the wounds were administrated with petrolatum, gelatin/Bletilla striata gelatin (GT/BSGT), or GT/BSGT plus PNS. We found that PNS signally facilitated wound healing and matrix remodeling in vivo. In conclusion, our study verified that PNS improved wound healing in hyperglycemic rats via promoting endothelial cell proliferation, invasion, migration, angiogenesis, suppressing cell apoptosis and oxidative damage, and activating the GSK-3β/β-catenin pathway.

show abstract

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Panax notoginseng saponins improves healing of high glucose‐induced wound through the GSK‐3β/β‐catenin pathway

Lei

Gao

Duan

et al. 2022

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

“…PNS can promote the proliferation of hematopoietic progenitor cells and modulate T cell immune functions ( 19 ). PNS can inhibit the oxidative stress of immune cells when infected with PCV2 ( 20 ). At present, research on the anti-viral activity of PNS is still in its infancy, and its immunomodulatory effects have not been evaluated.…”

Section: Introductionmentioning

confidence: 99%

Panax Notoginseng Saponins Suppress Type 2 Porcine Reproductive and Respiratory Syndrome Virus Replication in vitro and Enhance the Immune Effect of the Live Vaccine JXA1-R in Piglets

Yu²,

Wang³

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses the innate immune response in the host, reducing and delaying neutralizing antibody production against PRRSV infection and promoting viral infection. Here, we aimed to assess the potential of Panax notoginseng saponins (PNS) for improving the immune response exerted upon PRRSV-2-modified live virus (MLV) vaccine administration. Thirty piglets were randomly divided into six groups. Group 1 piglets were injected with medium 0 days post vaccination (dpv). Group 2 piglets were fed PNS 0–28 dpv. Group 3 and group 4 piglets were administered the JXA1-R vaccine 0 dpv. Group 4 piglets were also fed PNS 0–28 dpv. Group 1–4 piglets were challenged intranasally with the PRRSV JXA1 strain 28 dpv. Group 5 piglets were fed with PNS without challenge. Group 6 piglets served as controls. During the experiment, the samples were collected regularly for 49 days. Compared with group 1 piglets, group 3 piglets showed significantly reduced viremia and clinical scores, and significantly increased average daily gain (ADWG). Compared with group 3 piglets, group 4 piglets showed significantly improved neutralizing antibody titers, IFN-α and IFN-β mRNA expression, and significantly decreased viremia and viral load in the lungs and lymph nodes, but did not demonstrate any further improvement in PRRSV-specific antibody titer, rectal temperature, ADWG, or clinical scores. PNS upregulates neutralizing antibodies against PRRSV-2 and enhances the expression of IFN-α and IFN-β, which may reduce PRRSV viremia upon PRRSV-2 MLV vaccine administration. PNS may serve as an effective immunomodulator for boosting the immune defense against PRRSV.

show abstract

“…e Huangjia Ruangan granule is a pure Chinese medicine compound preparation, which is composed of Hedysarum Multijugum Maxim (Huangqi, HQ), Trionyx sinensis Wiegmann (Biejia, BJ), Radix Puerariae (Gegen, GG), Radix Bupleuri (Chaihu, CH), Ganoderma (Lingzhi, LZ), Radix Paeoniae Rubra (Chishao, CS), Radix Salviae (Danshen, DS), Panax Notoginseng (Sanqi, SQ), Abri Herba (Jigucao, JGC), and Phyllanthus urinaria L (YeXiaZhu, YXZ). HQ and CH are used to ameliorate liver injury [14,15]; BJ can soften and disperse knots; LZ, CS, DS, and SQ can promote the blood circulation and have antioxidant and anti-inflammatory effects [16][17][18][19]; GG, JGC, and YXZ can detoxify damp-heat residual toxins [5,20,21]. e combination of these drugs exerts a variety of pharmacological effects, such as antioxidation, anti-inflammatory, and antifibrosis while protecting the liver and relieving depression.…”

Section: Introductionmentioning

confidence: 99%

Huangjia Ruangan Granule Inhibits Inflammation in a Rat Model with Liver Fibrosis by Regulating TNF/MAPK and NF-κB Signaling Pathways

Cai

Wang²,

Zhang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

The Huangjia Ruangan granule (HJRG) is a clinically effective Kampo formula, which has a significant effect on liver fibrosis and early liver cirrhosis. However, the mechanism underlying HJRG in treating liver fibrosis remains unclear. In this study, carbon tetrachloride (CCl4) was used to induce liver fibrosis in rats to clarify the effect of HJRG on liver fibrosis and its mechanism. Using network pharmacology, the potential mechanism of HJRG was initially explored, and a variety of analyses were performed to verify this mechanism. In the liver fibrosis model, treatment with HJRG can maintain the liver morphology, lower the levels of AST and ALT in the serum, and ameliorate pathological damage. Histopathological examinations revealed that the liver structure was significantly improved and fibrotic changes were alleviated. It can effectively inhibit collagen deposition and the expression of α-SMA, reduce the levels of the rat serum (HA, LN, PC III, and Col IV), and inhibit the expression of desmin, vimentin, and HYP content in the liver. Analyzing the results of network pharmacology, the oxidative stress, inflammation, and the related pathways (primarily the TNF signaling pathway) were identified as the potential mechanism of HJRG against liver fibrosis. Experiments confirmed that HJRG can significantly increase the content of superoxide dismutase and glutathione and reduce the levels of malondialdehyde and myeloperoxidase in the rat liver; in addition, HJRG significantly inhibited the content of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) and reduced the expression of inflammatory regulators (Cox2 and iNOS). Meanwhile, treatment with HJRG inhibited the phosphorylation of NF-κB P65, IκBα, ERK, JNK, and MAPK P38. Moreover, HJRG treatment reversed the increased expression of TNFR1. The Huangjia Ruangan granule can effectively inhibit liver fibrosis through antioxidation, suppressing liver inflammation by regulating the TNF/MAPK and NF-κB signaling pathways, thereby preventing the effect of liver fibrosis.

show abstract

The effect ofPanax notoginsengsaponins on oxidative stress induced by PCV2 infection in immune cells:in vitroandin vivostudies

Cited by 16 publications

References 40 publications

Panax notoginseng saponins improves healing of high glucose‐induced wound through the GSK‐3β/β‐catenin pathway

Panax notoginseng saponins improves healing of high glucose‐induced wound through the GSK‐3β/β‐catenin pathway

Panax Notoginseng Saponins Suppress Type 2 Porcine Reproductive and Respiratory Syndrome Virus Replication in vitro and Enhance the Immune Effect of the Live Vaccine JXA1-R in Piglets

Huangjia Ruangan Granule Inhibits Inflammation in a Rat Model with Liver Fibrosis by Regulating TNF/MAPK and NF-κB Signaling Pathways

Contact Info

Product

Resources

About