The Effect of <i>Monascus</i> Secondary Polyketide Metabolites, Monascin and Ankaflavin, on Adipogenesis and Lipolysis Activity in 3T3-L1

Jou, Pey-Chyi; Ho, Bing‐Ying; Hsu, Ya-Wen; Pan, Tzu‐Ming

doi:10.1021/jf103121c

Cited by 80 publications

(67 citation statements)

References 34 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, monascin and ankaflavin were also found to reduce the intracellular accumulation of triglycerides, suppress the lipogenesis of mature adipocytes, and promote the breakdown of intracellular lipid droplets and the subsequent release of glycerin. Additionally, these two ingredients reduced extracellular LPL activities, thereby reducing in intracellular triglyceride synthesis (Jou et al 2010). In another animal experiment, monascin and ankaflavin were proven to suppress preadipocyte differentiation by suppressing the C/EBPα, C/EBPβ, and PPARγ expressions.…”

Section: Increasing Functional Metabolite Productionmentioning

confidence: 98%

The advantages of deep ocean water for the development of functional fermentation food

Lee¹

2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Deep ocean water (DOW) is obtained from 600 m below the sea surface. In recent years, DOW has been applied in the development of fermentation biotechnologies and functional foods. DOW is rich in trace minerals, comprises multiple physiological and health functions, and is able to promote microbe growth; therefore, the application of DOW directly benefits the development of the fermentation industry and functional foods. This study integrated the current health functions and applications of DOW with the latest results from studies related to fermentation biotechnology. Subsequently, the influence of applying DOW in fermented functional food development and the effects in health function improvements were summarized. According to the previous studies, the main reasons for the increased effect of fermented functional foods through the application of DOW are increased generation of functional metabolite contents in the microbes, intrinsic health functions of DOW, and the microbial use of mechanisms of converting the absorbed inorganic ions into highly bioavailable organic ions for the human body. These combined advantages not only enhance the health functions of fermentation products but also provide fermentation products with the intrinsic health functions of DOW.

show abstract

Section: Increasing Functional Metabolite Productionmentioning

confidence: 98%

The advantages of deep ocean water for the development of functional fermentation food

Lee¹

2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Obesity arises from an imbalance in energy intake and energy expenditure that eventually leads to the pathological growth of adipocytes [1]. Adipocytes are the major cellular component of fatty tissue.…”

Section: Introductionmentioning

confidence: 99%

Blueberry Peel Extracts Inhibit Adipogenesis in 3T3-L1 Cells and Reduce High-Fat Diet-Induced Obesity

et al. 2013

View full text Add to dashboard Cite

This study examined the anti-obesity effect and mechanism of action of blueberry peel extracts (BPE) in 3T3-L1 cells and high-fat diet (HFD)-induced obese rats. The levels of lipid accumulation were measured, along with the changes in the expression of genes and proteins associated with adipocyte differentiation in 3T3-L1 cells. Evidenced by Oil-red O staining and triglyceride assay, BPE dose-dependently inhibited lipid accumulation at concentrations of 0, 50, and 200 µg/ml. BPE decreased the expression of the key adipocyte differentiation regulator C/EBPβ, as well as the C/EBPα and PPARγ genes, during the differentiation of preadipocytes into adipocytes. Moreover, BPE down-regulated adipocyte-specific genes such as aP2 and FAS compared with control adipocytes. The specific mechanism mediating the effects of BP revealed that insulin-stimulated phosphorylation of Akt was strongly decreased, and its downstream substrate, phospho-GSK3β, was downregulated by BPE treatment in 3T3-L1 cells. Together, these data indicated that BP exerted anti-adipogenic activity by inhibiting the expression of PPARγ and C/EBPβ and the Akt signaling pathway in 3T3-L1 adipocytes. Next, we investigated whether BP extracts attenuated HFD-induced obesity in rats. Oral administration of BPE reduced HFD-induced body weight gain significantly without affecting food intake. The epididymal or perirenal adipose tissue weights were lower in rats on an HFD plus BPE compared with the tissue weights of HFD-induced obese rats. Total cholesterol and triglyceride levels in the rats fed BPE were modestly reduced, and the HDL-cholesterol level was significantly increased in HFD plus BP-fed rats compared with those of HFD-fed rats. Taken together, these results demonstrated an inhibitory effect of BP on adipogenesis through the down-regulation of C/EBPβ, C/EBPα, and PPARγ and the reduction of the phospho-Akt adipogenic factor in 3T3-L1 cells. Moreover, BPE reduced body weight gain and inhibited fat accumulation in an HFD-induced animal model of obesity.

show abstract

“…Rice fermented with the fungus, Monascus purpureus Went (Monascaceae) (red mould rice, RMR) NTU 568, has been reported to be effective for the management of hypercholesterolemia, diabetes, hypertension, antifatigue, obesity, Alzheimer's disease, and for the prevention of cancer (Li et al 1998; Lee et al 2006;Wang et al 2006;Wu et al 2009;Jou et al 2010;Lee et al 2010;Shi & Pan 2010). In our previous studies, extracts of M. purpureus NTU 568-fermented rice inhibited amyloid b (Ab)-induced neurocytotoxicity through the cooperative antioxidant and anti-inflammatory mechanisms (Lee et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effects of dimerumic acid and deferricoprogen fromMonascus purpureusNTU 568-fermented rice against 6-hydroxydopamine-induced oxidative stress and apoptosis in differentiated pheochromocytoma PC-12 cells

Tseng

Hsu

Pan

2016

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

Pan (2016) Neuroprotective effects of dimerumic acid and deferricoprogen from Monascuspurpureus NTU 568-fermented rice against 6-hydroxydopamine-induced oxidative stress and apoptosis in differentiated pheochromocytoma PC-12 cells, Pharmaceutical Biology, 54:8, 1434-1444, DOI: 10.3109/13880209.2015 Objective The effects of DMA and DFC on 6-hydroxydopamine (6-OHDA)-induced cytotoxicity and potential protective mechanisms in differentiated PC-12 pheochromocytoma cells were investigated. Materials and methods DMA (0-60 mM) or DFC (0-10 mM) was co-treated with 6-OHDA (200 mM, 24 h exposure) in differentiated PC-12 cells. Cell viability and intercellular reactive oxygen species (ROS) were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 2 0 ,7 0 -dichlorofluorescein-diacetate (DCFH-DA) assays, respectively. Cell apoptosis was determined by DNA fragmentation analysis and propidium iodide staining by flow cytometry. Western blot analysis was used to measure the levels of cell protein expression. Results DMA and DFC significantly increased cell viability to 72% and 81% in 6-OHDA-induced differentiated PC-12 cell cultures, respectively. Furthermore, DMA and DFC reduced 6-OHDAinduced formation of extracellular and intercellular ROS by 25% and 20%, respectively, and decreased NADPH oxidase-2 expression in differentiated PC-12 cells. DMA and DFC inhibited 6-OHDA-induced apoptosis and decreased activation of caspase-3 via regulation of Bcl-2-associated X protein (Bax) and Bcl-2 protein expression in differentiated PC-12 cells. Conclusion DMA and DFC may protect against 6-OHDA toxicity by inhibiting ROS formation and apoptosis. These results showed that the metabolites from M. purpureus NTU 568 fermentation were potential therapeutic agents for PD induced by oxidative damage and should be encouraged for further research. ARTICLE HISTORY

show abstract

The Effect of Monascus Secondary Polyketide Metabolites, Monascin and Ankaflavin, on Adipogenesis and Lipolysis Activity in 3T3-L1

Cited by 80 publications

References 34 publications

The advantages of deep ocean water for the development of functional fermentation food

The advantages of deep ocean water for the development of functional fermentation food

Blueberry Peel Extracts Inhibit Adipogenesis in 3T3-L1 Cells and Reduce High-Fat Diet-Induced Obesity

Neuroprotective effects of dimerumic acid and deferricoprogen fromMonascus purpureusNTU 568-fermented rice against 6-hydroxydopamine-induced oxidative stress and apoptosis in differentiated pheochromocytoma PC-12 cells

Contact Info

Product

Resources

About