The effect of Medicago sativa extract and light on skin hypopigmentation disorders in C57/BL6 mice

Abstract

Vitiligo is a common depigmentation skin disease that affects the quality of life in many patients. This study aims to investigate the effect of Medicago sativa methanol extract on the treatment of skin hypopigmentation disorders. Antioxidant activity and phytochemical constituents of the extract were determined using DDPH assay, Folin-Ciocalteu, AlCl 3 and HPLC-MS/MS analysis. Oil in water (o/w) creams were prepared to contain the methanolic extract, and applied to hydroquinone-induced depigmentation in vivo model and further challenged in combination with UVA light exposure. Skin and hair colors were visually scored and evaluated at different time intervals, and histopathological examinations of skin layers and hair follicles were performed. Results showed total phenolic content of 187.70 mg/g, equivalent to gallic acid, and total avonoid content of 21.97 mg/g, equivalent to quercetin.Most interestingly, the extract showed an antioxidant percent inhibition activity of 71%. Moreover, the HPLC-MS/MS detection revealed the presence of 18 compounds including P-coumaric acid and antioxidants avonoids, of those are 7 compounds not previously detected in this species. The in vivo study showed a remarkable skin and hair pigmentation effect on plant extract treated groups, compared to the reference, placebo and control groups. Histopathological examinations showed the growth of coloured hair follicles in the dermis and epidermis layers of the extract treated mice. In conclusion, the study suggests the use of M. sativa extract in enhancing the pigmentation process in hypopigmented skin and hair if combined with UVA light. Therefore, M. sativa extract can be considered a potential treatment for vitiligo.

The Formulation and Evaluation of Deep Eutectic Vehicles for the Topical Delivery of Azelaic Acid for Acne Treatment

“…Such formulation (i.e., MCP116 with AzA) exhibited higher efficacy than the marketed product, since they yielded a larger inhibition zone diameter, which might be explained by the fact that MCP116 with AzA scattered faster in the medium due to its high spreadability and diffusion characteristics. The assessment of skin irritation or corrosive potential was based on the Draize Dermal Irritation Scoring model, following a methodology consistent with prior findings [44]. The results are summarized in Table 3, and skin responses were consistently observed throughout all stages of the dermal testing process.…”

Antioxidant Activity and UHPLC-MS/MS Characterization of Polyphenol and Nicotine Content of <em>Nicotiana glauca</em> Leaf Extracts: A Comparative Study of Conventional and Deep Eutectic Solvent Extraction Methods

“…The determination of total flavonoids was performed using a colorimetric method based on the formation of a complex flavonoid-aluminum, measured at a wavelength of 510 nm using a UVspectrophotometer as described by Ubaydee, et al, (2022) [33]. The results were expressed as (mg/mL) equivalents to quercetin.…”