The effect of hypoxia on term trophoblast: Hormone synthesis and release

Esterman, Abbie L.; Finlay, Thomas H.; Dancis, Joseph

doi:10.1016/s0143-4004(96)90041-7

Cited by 59 publications

(18 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hypoxic treatment of cells was carried out as described by Esterman et al [35]. Cultures of PMCs were incubated in sealed Plexiglas chambers (Belleco Glass Co., Vineland, NJ) containing a gas oxygen analyzer (Hudson RCI, Temecula, CA) and a beaker of water to maintain humidity.…”

Section: Hypoxic Treatmentmentioning

confidence: 99%

Glucocorticoid Enhances Transforming Growth Factor-β Effects on Extracellular Matrix Protein Expression in Human Placental Mesenchymal Cells1

Lee¹,

Ma²,

LaChapelle³

et al. 2004

Biology of Reproduction

View full text Add to dashboard Cite

Extracellular matrix (ECM) proteins synthesized by human placental mesenchymal cells (PMCs) provide structural support for the villus. Aberrant expression of ECM proteins by PMCs has been associated with intrauterine growth restriction (IUGR). To provide insight into the mechanisms of ECM protein regulation in the stroma of the placental villus, in the current study, we examined the interaction of glucocorticoid (GC) and transforming growth factor-beta (TGFbeta) in the modulation of ECM proteins in cultures of PMCs isolated from human term placentas. Initial results obtained by ELISA showed that combined treatment with dexamethasone (DEX) and TGFbeta enhanced oncofetal fibronectin (FFN) protein levels in serum-free culture medium severalfold in a dose-dependent manner. Northern blotting and real-time polymerase chain reaction (PCR) analyses revealed a similar enhancement in levels of FN mRNA in cells treated with TGFbeta and DEX. Real-time PCR results also revealed that DEX and TGFbeta enhanced collagen (Col) I and Col IV expression, but did not affect levels of Col III or laminin, indicative of selective stimulation of ECM proteins. Hypoxic treatment moderately enhanced FFN levels in control cells but not in those treated with DEX and TGFbeta. In contrast with the results obtained with PMCs, we noted that DEX treatment suppressed FFN levels in untreated and TGFbeta-treated cytotrophoblasts, suggesting that GC and TGFbeta modulate FFN expression in placenta in a cell-type-specific manner. We conclude that GC and TGFbeta are key regulators of ECM protein synthesis in PMCs, suggesting a role in modulating placental architecture in uncomplicated pregnancies and those associated with aberrant ECM protein expression.

show abstract

Section: Hypoxic Treatmentmentioning

confidence: 99%

Glucocorticoid Enhances Transforming Growth Factor-β Effects on Extracellular Matrix Protein Expression in Human Placental Mesenchymal Cells1

Lee¹,

Ma²,

LaChapelle³

et al. 2004

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…Strohmer et al (1997) demonstrated that hypoxia down-regulates continuous expression of hCG in chorioncarcinoma cells. Accordingly, Esterman et al (1996) found that isolated term trophoblasts in culture remained viable when exposed to severe hypoxia, and release of hCG was reduced to <10% when compared with trophoblasts exposed to normal levels of oxygen. Theoretically, abnormal vascular bed and implantation in pregnancies with placenta previa could cause differences in maternal serum screening analytes, but in view of our results, if such a link exists, it is subtle.…”

Section: Discussionmentioning

confidence: 99%

Maternal serum second trimester AFP and hCG in pregnancies with placenta previa

2000

View full text Add to dashboard Cite

This study was undertaken to investigate the association between placenta previa and Down syndrome screening analytes-alpha-fetoprotein (AFP) and hCG-during the second trimester. Measurements of maternal serum AFP and hCG concentrations were retrospectively analysed in relation to placenta previa in a cohort of 46 consecutive singleton pregnancies affected by placenta previa from January 1993 through December 1997 at the University Hospital of Kuopio, Finland, and then compared with those in healthy singleton control pregnancies (N=9560) from the same clinic over the same period of time. Geometric means of maternal serum AFP and hCG concentrations in pregnancies with placenta previa were 1.13 and 0. 85 multiples of the median (MoM), respectively. The mean maternal age was higher in the subjects than in the controls (30.9 years compared with 28.8 years) (p<0.01). In relation to Down syndrome risk assessment, the pattern of the two markers together with maternal age indicated high risk as often in the study subjects as in the controls. Even though the maternal serum AFP and hCG differences were not statistically significant, they may be of some clinical importance, and further studies are needed to evaluate whether placental site should be taken into account in maternal serum screening.

show abstract

“…In previous studies, the effects of low O 2 tension on hormone synthesis and release of term trophoblast have been reported. 28 Reduced se- …”

Section: Oxygen Tension Regulates Cell Functionsmentioning

confidence: 99%

“…28 While the exact regulatory mechanism is poorly understood, decreased functional activity may be part of the strategy that the placenta has evolved to adapt to conditions of low oxygen availability. 25 In the present study, ED 27 trophoblast-like cells maintained viability under low oxygen tension over a period of 20 days.…”

mentioning

confidence: 99%

Oxygen Tension Influences Proliferation and Differentiation in a Tissue-Engineered Model of Placental Trophoblast-Like Cells

Yang

Kniss

2001

Tissue Engineering

View full text Add to dashboard Cite

A considerable oxygen gradient exists in vivo, which exerts regulatory effects on tissue development and function. The objective of this study was to evaluate the feasibility of controlling cell proliferation and differentiation by regulating oxygen tension in a tissue-engineered bioreactor model. The effects of oxygen tension on proliferation and differentiation of first-trimester human trophoblast cells (known as ED(27) cells) were studied in a fiber-bed perfusion bioreactor system in which cells were grown in polyethylene terephthalate (PET) nonwoven fibrous matrix. By varying the oxygen tension between 2% and 20%, differential responses of trophoblasts in their proliferation and differentiation activities were observed. There was no significant difference in the rates of glucose consumption and lactate production, and lactate dehydrogenase (LDH) level in the culture media for both 2% and 20% oxygen tension cultures, indicating that cell metabolic activities were not limited by low oxygen tension. However, 2% oxygen stimulated cell proliferation but impeded the secretion of a functional hormone, 17beta-estradiol. In contrast, 20% oxygen tension reduced cell proliferation, but yielded higher hormone secretion. A step change in oxygen tension from 2% to 20% caused cells in the bioreactor to increase 17beta-estradiol secretion and shifted cell cycle from proliferation to differentiation, which were verified with the expression levels of cyclin B1 and p27(kip1). However, no significant response to a change from 6% to 20% oxygen tension was observed. It is concluded that changes in oxygen tension can be an effective strategy to control cell cycle and long-term tissue development. This work also demonstrated the important role of oxygen tension in regulating placental trophoblast tissue development and the feasibility of using the bioreactor under well-controlled physiological environment for tissue engineering applications.

show abstract

The effect of hypoxia on term trophoblast: Hormone synthesis and release

Cited by 59 publications

References 17 publications

Glucocorticoid Enhances Transforming Growth Factor-β Effects on Extracellular Matrix Protein Expression in Human Placental Mesenchymal Cells1

Glucocorticoid Enhances Transforming Growth Factor-β Effects on Extracellular Matrix Protein Expression in Human Placental Mesenchymal Cells1

Maternal serum second trimester AFP and hCG in pregnancies with placenta previa

Oxygen Tension Influences Proliferation and Differentiation in a Tissue-Engineered Model of Placental Trophoblast-Like Cells

Contact Info

Product

Resources

About