The effect of hypoxia-mimicking responses on improving the regeneration of artificial vascular grafts

Rafique, Muhammad; Wei, Tingting; Sun, Qiqi; Midgley, Adam C.; Huang, Ziqi; Wang, Ting; Shafiq, Muhammad; Zhi, Dengke; Si, Jianghua; Yan, Hongyuan; Kong, Deling; Wang, Kai

doi:10.1016/j.biomaterials.2021.120746

Cited by 72 publications

(43 citation statements)

References 73 publications

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“…In this environment, M2 macrophages are firstly distributed, [ 38 ] and macrophages stimulated by the hypoxic environment directly regulate angiogenesis in the hypoxic area by secreting pro‐angiogenic factors. [ 39 ] In accordance with our findings, Arg1, CD206 and IL‐10, the markers of M2 macrophages, were upregulated in day 3 after surgery (Figure 6A), indicating the gathering of the cells. The M2 macrophages can secrete a variety of pro‐angiogenic factors, such as VEGF, TGF‐β, PDGF, ANG, bFGF, etc., chemokines, such as CXCL2, CXCL4, CXCL12, etc., and promote angiogenesis.…”

Section: Resultssupporting

confidence: 90%

Biomimetic Lamellar Chitosan Scaffold for Soft Gingival Tissue Regeneration

Feng

Gao

et al. 2021

Adv Funct Materials

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Mucogingival surgery has become a common procedure for soft gingival tissue reparation in dental clinical practice, which mainly relies on autograft or commercial collagen membranes (CM). However, the autograft faces grand challenges in source availability and long‐term post‐surgery pain management, and the CM is restricted by its poor mechanical properties in an aqueous environment. Here, it is reported that a bio‐inspired lamellar chitosan scaffold (LCS) with long range ordered porous structure, manufactured through a bidirectional freezing method, can serve as a promising gingival tissue engineering material. The LCS not only exhibits excellent mechanical properties in the hydrated state but also accelerates vessel formation and soft tissue regeneration in vivo. Most interestingly, the LCS is found to be capable of inducing macrophage differentiation to M2 macrophages, which is thought to play an important role in tissue regeneration. These advantages combined with its easy and low‐cost preparation process make the LCS a promising candidate for dental clinical applications.

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Section: Resultssupporting

confidence: 90%

Biomimetic Lamellar Chitosan Scaffold for Soft Gingival Tissue Regeneration

Feng

Gao

et al. 2021

Adv Funct Materials

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“…In this study, chiral hybrid scaffolds were constructed by blending electrospinning using PCL and the chiral molecule D(L)PHEG. The PCL component of the scaffolds can provide mechanical properties to maintain structural integrity, but its ion-inert properties limit its extensive use in vascular regeneration ( Rafique et al, 2021 ). To overcome the inert property, PCL is always used with other biomaterials for functional modification ( Yue et al, 2015 ; Yang et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Use of Electrospun Phenylalanine/Poly-ε-Caprolactone Chiral Hybrid Scaffolds to Promote Endothelial Remodeling

Sun

Hou

Sun

et al. 2021

Front. Bioeng. Biotechnol.

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The fabrication of tissue-engineered vascular grafts to replace damaged vessels is a promising therapy for cardiovascular diseases. Endothelial remodeling in the lumen of TEVGs is critical for successful revascularization. However, the construction of well-functioning TEVGs remains a fundamental challenge. Herein, chiral hybrid scaffolds were prepared by electrospinning using D/L-phenylalanine based gelators [D(L)PHEG] and poly-ε-caprolactone (PCL). The chirality of scaffolds significantly affected the endothelial remodeling progress of TEVGs. Compared with L-phenylalanine based gelators/poly-ε-caprolactone (L/PCL) and PCL, D-phenylalanine based gelators/poly-ε-caprolactone (D/PCL) scaffolds enhanced cell adhesion, and proliferation and upregulated the expression of fibronectin-1, and vinculin. These results suggests that chiral hybrid scaffolds can promote endothelial remodeling of TEVGs by upregulating adhesion-associated protein levels. This study offers an innovative strategy for endothelial remodeling of TEVGs by fabricating chiral hybrid scaffolds, and provides new insight for the treatment of cardiovascular diseases.

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“…An advantage of using synthetic polymers, such as PCL, PLCL, and PLGA is that good control can be exercised over their synthesis and modification ( Cameron and Shaver, 2011 ). Because synthetic polymers lack bioactivity and cell recognition factors, different types of bioactive factors, including growth factors and biologics, can be incorporated into them ( Rafique et al, 2021 ). The second type of biomaterials, designated as natural biomaterials, is derived from natural sources such as polysaccharides and proteins.…”

Section: Local Presentation Of Bioactive Cues To Enhance Stem Cell Survival and Retentionmentioning

confidence: 99%

Mechanobiological Strategies to Enhance Stem Cell Functionality for Regenerative Medicine and Tissue Engineering

Shafiq

Ali

Han

et al. 2021

Front. Cell Dev. Biol.

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Stem cells have been extensively used in regenerative medicine and tissue engineering; however, they often lose their functionality because of the inflammatory microenvironment. This leads to their poor survival, retention, and engraftment at transplantation sites. Considering the rapid loss of transplanted cells due to poor cell-cell and cell-extracellular matrix (ECM) interactions during transplantation, it has been reasoned that stem cells mainly mediate reparative responses via paracrine mechanisms, including the secretion of extracellular vesicles (EVs). Ameliorating poor cell-cell and cell-ECM interactions may obviate the limitations associated with the poor retention and engraftment of transplanted cells and enable them to mediate tissue repair through the sustained and localized presentation of secreted bioactive cues. Biomaterial-mediated strategies may be leveraged to confer stem cells enhanced immunomodulatory properties, as well as better engraftment and retention at the target site. In these approaches, biomaterials have been exploited to spatiotemporally present bioactive cues to stem cell-laden platforms (e.g., aggregates, microtissues, and tissue-engineered constructs). An array of biomaterials, such as nanoparticles, hydrogels, and scaffolds, has been exploited to facilitate stem cells function at the target site. Additionally, biomaterials can be harnessed to suppress the inflammatory microenvironment to induce enhanced tissue repair. In this review, we summarize biomaterial-based platforms that impact stem cell function for better tissue repair that may have broader implications for the treatment of various diseases as well as tissue regeneration.

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The effect of hypoxia-mimicking responses on improving the regeneration of artificial vascular grafts

Cited by 72 publications

References 73 publications

Biomimetic Lamellar Chitosan Scaffold for Soft Gingival Tissue Regeneration

Biomimetic Lamellar Chitosan Scaffold for Soft Gingival Tissue Regeneration

Use of Electrospun Phenylalanine/Poly-ε-Caprolactone Chiral Hybrid Scaffolds to Promote Endothelial Remodeling

Mechanobiological Strategies to Enhance Stem Cell Functionality for Regenerative Medicine and Tissue Engineering

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