The effect of hyposmotic and sodium chloride hyperosmotic environments on the secretion and synthesis of prolactin from human decidua in vitro

Andersen, Jesper Rye; Borggaard, Birgit; Ørnvold, Kim; Knoth, Mogens

doi:10.1530/acta.0.1000623

Cited by 10 publications

(8 citation statements)

References 11 publications

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“…Further, the proportion of living cells found after trypan blue staining is similar that reported earlier (Tomita et al 1982) and we suspect that the main cell death occurs during preparation of the tissue before incubation. In our first report the slightly decreasing glucose consumption and the histolo¬ gical appearance were reported earlier (Andersen et al 1982) and we conclude that almost all the tissue stays alive and metabolically active during the successive incubations.…”

Section: Discussionsupporting

confidence: 81%

“…A detailed example of analysis of variance using the cross-over design has been given previously (Andersen et al 1982) and in the same way the experiments of this report showed significant flask to flask variation in more than half of the experi¬ ments. Fig.…”

Section: Resultssupporting

confidence: 70%

“…The decidua/chorion was isolated and prepared as earlier described (Andersen et al 1982). Four or 5 flasks (40 ml tissue culture flasks, Nunc), each containing 200-400 mg wet decidua/chorion, were prepared from each foetal membrane.…”

Section: Methodsmentioning

confidence: 99%

“…We have earlier presented results indicating that the osmolality and/or the ionic concentrations of the incubation media could affect the production and secretion of Prl from human decidua in vitro (Andersen et al 1982). The purpose of this study was to evaluate whether this effect was due to changes in the osmolality or to changes in the concentration of specific ions.…”

mentioning

confidence: 98%

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The dependence of human decidual prolactin production and secretion on the osmotic environment in vitro

Andersen

Borggaard

Schroeder

et al. 1984

Acta Endocrinologica

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Human decidual tissue from uncomplicated term pregnancies was incubated in vitro using a cross\x=req-\ over design of incubation lasting for 72 h. The decidual tissue of each membrane was added sequentially to media with the osmolalities 252, 315 and 387 mmol/kg, and the different osmolalities were in 33 experiments induced by changing the concentration either of sodium chloride, potassium chloride, mannitol, sucrose, or choline chloride.At 387 mmol/kg all substances elicited a significant increase in Prl secretion compared with the 315 mmol/kg media (14\p=n-\27%) or the 252 mmol/kg media (26\p=n-\46%). When the sodium chloride, potassium chloride, sucrose, and choline chloride media at 387 mmol/kg were compared in another 7 experiments, potassium chloride increased Prl secretion more effectively than the others (10%, P < 0.05).The incubation cross-over design did not permit determination of the decidual Prl content after incubation in each of the various media, but in 28 experiments the mean decidual Prl content was 12.7 mIU per 100 mg dry tissue (range 4\p=n-\30) before and 8.7 mIU per 100 mg dry tissue (1\ p=n-\ 17) after the experiments. The mean amount of Prl secreted during the successive incubations was 152.2 mIU per 100 mg dry tissue (19\p=n-\672) which showed the secretion to be a result of continued Prl production. The effect of osmolality was independent of the basal production rate. These results indicate that the intracellular ionic concentrations, probably of potassium ion or of chloride ion, are of importance in the regulation of the synthesis and secretion of decidual Prl in vitro.Please send proofs to: Jesper Rye Andersen,

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Section: Discussionsupporting

confidence: 81%

Section: Resultssupporting

confidence: 70%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 98%

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The dependence of human decidual prolactin production and secretion on the osmotic environment in vitro

Andersen

Borggaard

Schroeder

et al. 1984

Acta Endocrinologica

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“…Although an agreement is far from being reached [Adler et al, 1975;Berl et al, 1976;Epstein et al, 1977;Dearlove, 1981;Bliss and Lote, 1982], several reports have proposed an association between PRL and osmoregula tion, the hormone being osmotically regu lated [Buckman and Peake, 1973;Buckman et al, 1973;Relkin, 1974;Andersen et al, 1982] , or acting on osmotic regulation [Dharmamba et al, 1967;Josimovich et al, 1977;Ensor, 1978;Leontic et al, 1979;Gopalakrishnan et al, 1980;Mor ley et al, 1981]. Since loss of endogenous heat generated by contracting muscles can lead to marked blood fluid shifts [Senay, 1979], we postu lated that prolactinemic changes observed during exercise could be associated with vari ations in core temperature and/or in blood osmolality.…”

Section: Introductionmentioning

confidence: 99%

Exercise-Induced Blood Prolactin Variations in Trained Adult Males: A Thermic Stress More than an Osmotic Stress

Brisson¹,

Audet

Ledoux

et al. 1986

Horm Res

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Blood prolactin (PRL) variations have been linked to temperature and osmotic changes in several species. The latter factors are here explored to better understand blood PRL responses frequently induced during physical exercise. Since body heat generated by exercise can lead to marked body fluid shifts, it was postulated that PRL changes observed during exercise could be associated with variations in body temperature and/or blood osmolality (OSM). A wide range (38.5–40.5°C) of rectal temperatures (Tr; used here to appreciate core temperatures) were theoretically selected and randomly assigned as targets to male runners. Measured by thermistor probe, target Tr were obtained by a combination of factors: (a) ↑ heat production by treadmill running, and (b) ⇓ heat losses by appropriate clothing (⇓ evaporation) in warmed (⇓ radiation) and hypo ventilated (⇓ convection) laboratory conditions. For each subject, target Tr was attained not prior to 30 min after initiation of running, and had to be maintained for at least 10 min, for a mean ( ± SD) running time of 52.6 ± 10.0 min. In a first protocol, hypohydration was provoked in 26 runners (23.9 ± 4.7 years) by total restriction of water intake. In a second protocol (10 different runners: 22.3 ± 3.3 years), euhydration was maintained by water intake (20 ml/kg body weight). Venous blood was sampled at rest before and immediately after the run. PRL was assayed by RIA; OSM was measured by freezing point depression; sodium was analyzed by flame photometry. At rest, before the heat-producing exercise, mean PRL values were 9.4 ± 3.4 ng/ml for both eu/hypohydrated groups. In the hypohydrated runners, exercise-induced hyperthermia was significantly (r = 0.82; p < 0.0005) associated with blood PRL responses. Moreover, these changes in Tr were also significantly (r = 0.54; p < 0.0025) related to changes in OSM, the latter variations being mostly explained (78 %) by the accompanying hypernatremia. In the euhydrated group of runners, the hyperthermic exercise failed to induce significant changes in OSM (r = 0.22; p > 0.15) and, as expected, variations in blood sodium levels were also not significant under these conditions. However, hyperthermic running in these iso-osmolar conditions did not prevent blood PRL levels from rising (r = 0.77; p < 0.0005). It was thus concluded that, in male trained runners, exercise-induced blood PRL responses could be derived more from thermic than from osmolar stresses.

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Amniotic fluid prolactin, decidual prolactin content and decidual prolactin secretion into HYPO-, ISO-, and sodium chloride hyperosmotic mediain vitro in pregnant diabetics at term

et al. 1986

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This study evaluated in pregnant women with diabetes mellitus (DM) the prolactin (PRL) concentration in amniotic fluid (A-PRL), the initial decidual PRL content (D-PRL), and the decidual PRL secretion (D-PRL-s) in vitro into isosmotic (315 mmol/kg), hyperosmotic (426 mmol/kg) and hyposmotic (252 mmol/kg) media. Decidual tissue was collected at term from 18 normal pregnancies and from 23 women with DM (White's classification: 7 of class A (without insulin treatment) and 16 of the classes B-F. Twelve of the women with DM had signs of hydramnion. Amniotic fluid specimens were collected from 52 normal pregnancies and 17 of the women with DM. No significant difference was found between normals and diabetics when D-PRL, D-PRL-s into isosmotic medium and A-PRL were compared. No trends could be detected when the insulin treated women were grouped according to White's classification. The increment of D-PRL-s into hyperosmotic medium was 15% in normals (p less than 0.001 compared with isosmotic medium), in the DM group 23% for the non-insulin-treated women (p less than 0.05 when compared with normals) and 25% for the insulin treated women (p less than 0.01). In normals the hyposmotic medium reduced D-PRL-s by secretion 19% (p less than 0.001 compared with isosmotic medium) but no significant difference was observed between normals and diabetic groups (p greater than 0.10). In the group of diabetics no significant difference was found in any of the PRL quantities investigated between those with and without hydramnion.(ABSTRACT TRUNCATED AT 250 WORDS)

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The effect of hyposmotic and sodium chloride hyperosmotic environments on the secretion and synthesis of prolactin from human decidua in vitro

Cited by 10 publications

References 11 publications

The dependence of human decidual prolactin production and secretion on the osmotic environment in vitro

The dependence of human decidual prolactin production and secretion on the osmotic environment in vitro

Exercise-Induced Blood Prolactin Variations in Trained Adult Males: A Thermic Stress More than an Osmotic Stress

Amniotic fluid prolactin, decidual prolactin content and decidual prolactin secretion into HYPO-, ISO-, and sodium chloride hyperosmotic mediain vitro in pregnant diabetics at term

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