The effect of hydroxyethyl starch and other plasma volume substitutes on endothelial cell activation; an in vitro study

Collis, Rachel; Collins, Peter William; Gutteridge, C. N.; Kaul, Arvind; Newland, Adrian C.; Williams, David M.; Webb, Andrew R.

doi:10.1007/bf02425053

Cited by 115 publications

(68 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HES also prevented von Willebrand factor release in cultured human umbilical vein endothelial cells and inhibited early endothelial cell activation and rapid P-selectin expression, although no effect on endothelial E-selectin or neutrophil CD11b/CD18 expression was noted. 19 Other studies suggest that HES may directly reduce the neutrophil chemotaxis through endothelial cell monolayers. 24 These mechanistic pathways may underlie the reduction in leukocyte-endothelial adherence we noted in postischemic brain in the present study, but further experiments are needed to confirm in vivo these and other proinflammatory signaling pathways on which HES might exert influence.…”

Section: Discussionmentioning

confidence: 99%

Hydroxyethyl Starch Reduces Leukocyte Adherence and Vascular Injury in the Newborn Pig Cerebral Circulation After Asphyxia

Kaplan

Park

Gonzales

et al. 2000

Stroke

View full text Add to dashboard Cite

Background and Purpose-Hydroxyethyl starch (HES) has beneficial effects on ischemic brain injury; however, its mechanism of action remains unclear. The present study was undertaken to test the hypothesis that HES can attenuate increases in leukocyte adherence and vascular permeability in the cerebral vasculature after global cerebral ischemia induced by asphyxia. Methods-Pial venular leukocyte adherence and permeability to sodium fluorescein were quantified in anesthetized newborn piglets by in situ fluorescence videomicroscopy through closed cranial windows during basal conditions and during 2 hours of reperfusion after global ischemia induced by 9 minutes of asphyxia. Experimental animals received HES after the asphyxial insult (10% HES 257/0.47, 600 mg/kg IV bolus 5 minutes after asphyxia, followed by 600 mg/kg per hour IV drip during reperfusion; nϭ9). Results-A progressive and significant (PϽ0.05) increase in adherent leukocytes was observed during the initial 2 hours of reperfusion after asphyxia compared with nonasphyxial controls. In this model, vascular injury, as determined by significant (PϽ0.05) increases in fluorescein permeability at 2 hours of reperfusion, is largely dependent on adherent leukocytes. HES significantly reduced (PϽ0.05) leukocyte adherence at 1 hour and 2 hours of reperfusion and reduced fluorescein permeability at 2 hours. HES did not change hematocrit or alter pial arteriolar diameter. Conclusions-These findings indicate that a vascular anti-inflammatory action may underlie the beneficial effects of HESin global cerebral ischemia secondary to asphyxia. Since this compound is well tolerated by patients, future preclinical and clinical studies may reveal improvements in functional outcome with the early introduction of this or similar agents after perinatal asphyxia or global ischemia. (Stroke. 2000;31:2218-2223.)Key Words: cerebral ischemia, global Ⅲ inflammation Ⅲ leukocytes Ⅲ reperfusion injury H ydroxyethyl starch (HES) is a clinically well-tolerated complex polysaccharide that has recently been used in the therapeutic treatment of stroke and vasospasm after subarachnoid hemorrhage. It is available in multiple preparations, each with different pharmacological characteristics based on concentration, molecular weight, degree of substitution, and C2/C6 hydroxyethylation ratio. While HES has shown a protective effect with reductions in infarct size and/or improvement in outcome in experimental models of ischemic injury in both central nervous system (CNS) 1-6 and peripheral tissues, 7-10 results of its use in clinical stroke trials have been less encouraging. [11][12][13] This may relate to the time window of administration of the compound relative to stroke onset, as well as the pharmacological characteristics of the particular HES preparation; nevertheless, clinical interest still remains, 14 particularly in situations of elevated intracranial pressure. 15 See Editorial Comment, page 2223The mechanistic basis of the beneficial effects of HES remains unclear. Postulated actions inc...

show abstract

Section: Discussionmentioning

confidence: 99%

Hydroxyethyl Starch Reduces Leukocyte Adherence and Vascular Injury in the Newborn Pig Cerebral Circulation After Asphyxia

Kaplan

Park

Gonzales

et al. 2000

Stroke

View full text Add to dashboard Cite

show abstract

“…It is likely that endothelial-related coagulation plays a significant role, as the starches diminish leucocyte adherence to the endothelium, as well as modify endothelial porosity in states of capillary leak [16,17]. However, these experiments throw no light on this aspect of coagulation, as thrombelastographic measurements exclude any consideration of plateletendothelial interactions.…”

Section: ó 2002 Blackwell Publishing Ltdmentioning

confidence: 99%

Coagulation effects of in vitro serial haemodilution with a balanced electrolyte hetastarch solution compared with a saline‐based hetastarch solution and lactated Ringer's solution*

et al. 2002

View full text Add to dashboard Cite

SummaryThe hydroxyethyl starches are a group of compounds that has been associated with impairment of coagulation when large volumes are administered. The thrombelastographÒ is commonly used to assess point-of-care whole blood coagulation. Little is known about the dose-response relationships of haemodilution, and it is reasonable to assume that a linear association exists. This may not be the case with altered electrolyte compositions of the fluids used for haemodilution. We have therefore conducted an in vitro study of haemodilution of human whole blood using lactated Ringer's solution and two high molecular weight hetastarches, one in a balanced salt solution, the other in a 0.9% saline solution. The thrombelastographÒ, commonly used for the assessment of the coagulation effects of synthetic colloids, was used as the coagulation assessment device. Serial haemodilution with hetastarch in a balanced salt solution demonstrated a biphasic response (of r-times and k-times, as well as alpha angles), with haemodilution in the 20-40% range causing enhanced coagulation, and higher degrees of dilution causing a decrease in overall coagulation performance. A similar picture was observed with lactated Ringer's solution, but only significantly so in alpha angles. Hetastarch in saline did not display this initial increased coagulability at mild to moderate dilutions. This biphasic response of lactated Ringer's solution and hetastarch in a balanced salt solution reflects the complex interaction of fluids and the coagulation system, and that these effects cannot be attributed to simple haemodilution. On the other hand, there was a linear decrease in maximum amplitude with haemodilution. Maximum amplitude was particularly affected by both starches, which is an expected finding in view of the known interaction between the hydroxyethyl starches and von Willebrand's factor.

show abstract

“…An in vitro study conducted by Collis et al (1994), observing the influence of hydroxyethyl starch on endothelial cells activity in culture, showed that there was inhibition of von Willebrand factor release, which comprised adhesion molecules stored in endothelial cells in group receiving HES. This resulted in significant decreased of von Willebrand factor (p = 0.01) compared to other plasma substitution fluids.…”

Section: Discussionmentioning

confidence: 99%

Effect of Hydroxyethyl Starch 200/0.5 on Von Willebrand Factor Serum Level and Activated Partial Thromboplastin Time (Aptt)

Atmaja¹,

Khotib²,

Rahardjo³

et al. 2016

FMI

View full text Add to dashboard Cite

show abstract

The effect of hydroxyethyl starch and other plasma volume substitutes on endothelial cell activation; an in vitro study

Abstract: these results suggest a possible beneficial role of hydroxyethyl starches in the inhibition of endothelial activation thus preventing neutrophil adhesion during sepsis syndrome.

Cited by 115 publications

References 20 publications

Hydroxyethyl Starch Reduces Leukocyte Adherence and Vascular Injury in the Newborn Pig Cerebral Circulation After Asphyxia

Hydroxyethyl Starch Reduces Leukocyte Adherence and Vascular Injury in the Newborn Pig Cerebral Circulation After Asphyxia

Coagulation effects of in vitro serial haemodilution with a balanced electrolyte hetastarch solution compared with a saline‐based hetastarch solution and lactated Ringer's solution*

Effect of Hydroxyethyl Starch 200/0.5 on Von Willebrand Factor Serum Level and Activated Partial Thromboplastin Time (Aptt)

Contact Info

Product

Resources

About