The Effect of Hydrolysis with Neutrase on Molecular Weight, Functional Properties, and Antioxidant Activities of Alaska Pollock Protein Isolate

Liu, Chuyi; Ma, Xiaoming; Che, Shuai; Wang, Changwei; Li, Bafang

doi:10.1007/s11802-018-3649-9

Cited by 9 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They further mentioned that protease type, hydrolysis time and the ratio of enzyme/substrate affected the digestion pattern of protein into peptides and amino acids. Liu et al (2018) observed that complete degradation of the Alaska pollock protein isolate by neutrase occurred at 40 to 240 mins of hydrolysis with the average molecular weights produced were less than 10 kDa. At the final hydrolysis time (240 mins), small peptides (500 Da) were produced.…”

Section: Molecular Weight Distributionmentioning

confidence: 99%

“…The use of neutrase with the total activity of 2.01x10 5 U produced DH of Alaska pollock hydrolysates of approximately. 25% and reached equilibrium at 160 mins hydrolysis (Liu et al, 2018). They stated that neutrase was the most efficient enzyme to improve protein solubility compared to six food-grade proteases.…”

Section: Degree Of Hydrolysis (Dh) and Peptide Contentmentioning

confidence: 99%

“…The use of neutrase to hydrolyze sea cucumber collagen from Acaudina molpadioides produced the highest antioxidant activity of hydrolysate compared to those produced using papain, pepsin and trypsin (Jin, Xu, Li, Zhang, & Xie, 2019). Neutrase was also reported as the best enzyme that produced hydrolysates efficiently with beneficial functional and bioactive characteristics (Liu, Ma, Che, Wang, & Li, 2018;Ou et al, 2010).…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

Fawzya¹,

Putra²,

Witarto³

et al. 2020

Squalen Bull. Marine Fisheries Postharvest Biotech.

View full text Add to dashboard Cite

Golden sea cucumber or locally known as “teripang emas” is one of Indonesia’s most popular sea cucumber and widely processed as functional food or supplement due to its bioactivities. The sea cucumber is often misidentified due to its morphological similarities with other Stichopus spp. This study aimed to identify the golden sea cucumber obtained from West Nusa Tenggara, Indonesia, by a molecular method and study the antioxidant activities of its collagen hydrolysates. The hydrolysates were produced by hydrolyzing acid collagen extract using neutrase for 30, 60, 120, 180, and 240 mins. The products were then analyzed for their degree of hydrolysis, peptide content, molecular weight distribution and radical scavenging activity by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. Results showed that hydrolysis for 180 mins was optimal in producing the highest peptide content, 12.79 ± 0.44 mg/mL, with a degree of hydrolysis (DH) of 55.2 ± 1.50%. However, the highest antioxidant activity (IC50 of 5.25 ± 0.15 mg/mL) was demonstrated after 60 mins hydrolysis with molecular weight (MW) ranged from less than 14.4 kDa to approximately 25 kDa. The hydrolysate might be categorized as a weak to moderate antioxidant. Based on the molecular identification, the golden sea cucumber had 99% similarities with Stichopus horrens and S. monotuberculatus.

show abstract

Section: Molecular Weight Distributionmentioning

confidence: 99%

Section: Degree Of Hydrolysis (Dh) and Peptide Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

Fawzya¹,

Putra²,

Witarto³

et al. 2020

Squalen Bull. Marine Fisheries Postharvest Biotech.

View full text Add to dashboard Cite

show abstract

“…Other marine sources for preparation of HC were obtained from cod protein hydrolysate ( Gadus morhua ) [103], Alaska pollock [104], and cartilage of spotless smooth hound [105]. The extracted biomaterials results were lower molecular weights (3–5 KDa) and antioxidative activity.…”

Section: Hydrolyzed Collagen: Sources and Applicationsmentioning

confidence: 99%

Hydrolyzed Collagen—Sources and Applications

et al. 2019

View full text Add to dashboard Cite

Hydrolyzed collagen (HC) is a group of peptides with low molecular weight (3–6 KDa) that can be obtained by enzymatic action in acid or alkaline media at a specific incubation temperature. HC can be extracted from different sources such as bovine or porcine. These sources have presented health limitations in the last years. Recently research has shown good properties of the HC found in skin, scale, and bones from marine sources. Type and source of extraction are the main factors that affect HC properties, such as molecular weight of the peptide chain, solubility, and functional activity. HC is widely used in several industries including food, pharmaceutical, cosmetic, biomedical, and leather industries. The present review presents the different types of HC, sources of extraction, and their applications as a biomaterial.

show abstract

“…Several studies on antioxidant activity have been conducted with HC from different sources such as pigs [4,21], cows [22], fish [23,24,25], and invertebrates like jellyfishes or sponges [26,27]. However, less is known about the properties of hydrolysed collagen extracted from ovine sources and its possible applications.…”

Section: Introductionmentioning

confidence: 99%

Hydrolysed Collagen from Sheepskins as a Source of Functional Peptides with Antioxidant Activity

León-López

Fuentes-Jiménez

Hernández-Fuentes

et al. 2019

IJMS

View full text Add to dashboard Cite

The extraction and enzymatic hydrolysis of collagen from sheepskins at different times of hydrolysis (0, 10, 15, 20, 30 min, 1, 2, 3 and 4 h) were investigated in terms of amino acid content (hydroxyproline), isoelectric point, molecular weight (Mw) by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method, viscosity, Fourier-transform infrared (FTIR) spectroscopy, antioxidant capacity by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, thermal properties (Differential Scanning Calorimetry) and morphology by scanning electron microscopy (SEM) technique. The kinetics of hydrolysis showed an increase in the protein and hydroxyproline concentration as the hydrolysis time increased to 4 h. FTIR spectra allowed us to identify the functional groups of hydrolysed collagen (HC) in the amide I region for collagen. The isoelectric point shifted to lower values compared to the native collagen precursor. The change in molecular weight and viscosity from time 0 min to 4 h promoted important antioxidant activity in the resulting HC. The lower the Mw, the greater the ability to donate an electron or hydrogen to stabilize radicals. From the SEM images it was evident that HC after 2 h had a porous and spongy structure. These results suggest that HC could be a good alternative to replace HC from typical sources like pigs, cows and fish.

show abstract

The Effect of Hydrolysis with Neutrase on Molecular Weight, Functional Properties, and Antioxidant Activities of Alaska Pollock Protein Isolate

Cited by 9 publications

References 37 publications

Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

Golden Sea Cucumber: Identification and the Antioxidant Activity of Its Collagen Hydrolysates

Hydrolyzed Collagen—Sources and Applications

Hydrolysed Collagen from Sheepskins as a Source of Functional Peptides with Antioxidant Activity

Contact Info

Product

Resources

About