The Effect of Hydrodynamics on Biological Materials

Thomas, C. R.; Zhang, Zhibing

doi:10.1007/978-94-017-0643-8_9

Cited by 23 publications

(15 citation statements)

References 47 publications

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“…Modern analysis of the impact of stresses generated by agitation on animal and bacterial cells is usually based on Kolmogorov"s theory of isotropic turbulence (Thomas and Zhang 1998;Nienow, 2006). This theory requires the flow in the vessel to be highly turbulent for it to be applicable.…”

Section: Resultsmentioning

confidence: 99%

Expansion of human mesenchymal stem cells on microcarriers

et al. 2011

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Section: Resultsmentioning

confidence: 99%

Expansion of human mesenchymal stem cells on microcarriers

et al. 2011

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“…Although air-lift, hollow-fiber, and fixed bed (for anchorage-dependent cells) bioreactors are also used, the majority of industrial processes use stirred-tank bioreactors, and for that reason, the discussion here has been limited to stirred tank bioreactors. The issues related to agitation and aeration are summarized in this section, although several excellent reviews (Prokop and Bajpai, 1992;Aunins and Henzler, 1993;Wu, 1995;Joshi et al, 1996;Thomas and Zhang, 1998) on this subject have been published and the reader is referred to these for detailed discussions.…”

Section: -6 Cell Culturementioning

confidence: 99%

“…As Thomas and Zhang (1998) point out, the term shear must be one of the most abused words in the biochemical engineering literature and has gained a nearly colloquial usage to imply any hydrodynamic effect on biological materials. Even the term shear damage is problematic.…”

Section: -61 Shear Damage and Kolmogorov's Theory Of Isotropic Turmentioning

confidence: 99%

Mixing in the Fermentation and Cell Culture Industries

Amanullah¹,

Buckland²,

Nienow

2003

Handbook of Industrial Mixing

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“…3 To understand and predict cell disruption, knowledge of the effects of fluid stress on the cell and of the material properties of the cell wall is required. [4][5][6] There may be different types of stress generation in different types of disruption equipment, but in all cases the response of the cell will be determined by the mechanical properties of the cell wall. Improvement in disruption has been the motivation of recent studies into the mechanical properties of yeast cells.…”

Section: Introductionmentioning

confidence: 99%

Determining the mechanical properties of yeast cell walls

Stenson

Hartley

Wang

et al. 2011

Biotechnology Progress

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The intrinsic cell wall mechanical properties of Baker's yeast (Saccharomyces cerevisiae) cells were determined. Force-deformation data from compression of individual cells up to failure were recorded, and these data were fitted by an analytical model to extract the elastic modulus of the cell wall and the initial stretch ratio of the cell. The cell wall was assumed to be homogeneous, isotropic, and incompressible. A linear elastic constitutive equation was assumed based on Hencky strains to accommodate the large stretches of the cell wall. Because of the high compression speed, water loss during compression could be assumed to be negligible. It was then possible to treat the initial stretch ratio and elastic modulus as adjustable parameters within the analytical model. As the experimental data fitted numerical simulations well up to the point of cell rupture, it was also possible to extract cell wall failure criteria. The mean cell wall properties for resuspended dried Baker's yeast were as follows: elastic modulus 185 ± 15 MPa, initial stretch ratio 1.039 ± 0.006, circumferential stress at failure 115 ± 5 MPa, circumferential strain at failure 0.46 ± 0.03, and strain energy per unit volume at failure 30 ± 3 MPa. Data on yeast cells obtained by this method and model should be useful in the design and optimization of cell disruption equipment for yeast cell processing.

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The Effect of Hydrodynamics on Biological Materials

Cited by 23 publications

References 47 publications

Expansion of human mesenchymal stem cells on microcarriers

Expansion of human mesenchymal stem cells on microcarriers

Mixing in the Fermentation and Cell Culture Industries

Determining the mechanical properties of yeast cell walls

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