The effect of hydroalcoholic extract of Urtica dioica on morphine withdrawal signs in male mice

Asadi, Asad; Maleki, Saeed

doi:10.15171/jhp.2018.34

Cited by 2 publications

(4 citation statements)

References 25 publications

(32 reference statements)

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“…Nonetheless, other studies have assessed and confirmed the safety of these extracts. Asadi and Abbasi Maleki demonstrated the LD 50 of hydroalcoholic extract U. dioica in mice as 2900 mg/kg during acute toxicity testing, much higher than the doses used in our experiment (52). Similarly, P. boissieriana extract given to mice models at doses of 500 and 1000 mg/kg showed no reported side effects or mortalities (21,53).…”

Section: Discussionmentioning

confidence: 42%

In Vitro Scolicidal Effect of Urtica dioica and Pyrus boissieriana Extracts Against Protoscoleces of Echinococcus granulosus

Goudarzi,

Alimi,

Zarghami

et al. 2024

Shiraz E-Med J

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Background: Cystic echinococcosis (CE) is one of the most important neglected diseases and a public health concern worldwide. Due to the poor efficacy of current drugs, novel therapeutic approaches are urgently needed. Objectives: This study evaluated the scolicidal effects of the hydroalcoholic extract of Urtica dioica and the chloroformic extract of Pyrus boissieriana on protoscoleces of CE cysts. Methods: Protoscoleces were aseptically aspirated from the livers of sheep naturally infected with CE cysts. To assess the scolicidal effect of these herbal extracts, various concentrations of both extracts (5, 10, 20, 40, and 80 mg/mL) were added to a suspension of 3 × 103 washed protoscoleces. After 10, 20, 30, 60, and 120 minutes of exposure, eosin stain was added to each tube, and the viability of protoscoleces was evaluated by flame cell motility under a light microscope, as well as impermeability to 0.1% eosin solution. All experiments were performed in triplicate. Results: The scolicidal effects of U. dioica extracts at a concentration of 80 mg/mL were 81% and 89% after 60 and 120 minutes of exposure, respectively. Almost the same results were obtained for P. boissieriana extracts at a concentration of 80 mg/mL (81.33% and 89% after 60 and 120 minutes of exposure, respectively), which were significantly different from the negative control group (P < 0.001). However, the extract of U. dioica exhibited stronger scolicidal effects compared to the extract of P. boissieriana at concentrations lower than 40 mg/mL (P < 0.001). Conclusions: The present findings indicate that both U. dioica and P. boissieriana extracts possess high protoscolicidal activities and could be used as alternative natural medicines in the treatment of CE. However, further studies are required to verify these findings through assessment in animal models and clinical subjects.

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Section: Discussionmentioning

confidence: 42%

In Vitro Scolicidal Effect of Urtica dioica and Pyrus boissieriana Extracts Against Protoscoleces of Echinococcus granulosus

Goudarzi,

Alimi,

Zarghami

et al. 2024

Shiraz E-Med J

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“…With this in mind, previous studies have demonstrated that the GABAergic system performs a pivotal function in opioid addiction. Moreover, studies have shown that positive modulators on the benzodiazepine site in the GABAA receptors (e.g., diazepam) and selective GABAA receptor agonists (e.g., muscimol) pacify morphine withdrawal signs (6,19,20,27).…”

Section: Discussionmentioning

confidence: 99%

“…In order to induce morphine tolerance or dependence, this compound was administered i.p. three times daily at 8 a.m. (50 mg/kg), 12 p.m. (50 mg/kg), and 4 p.m. for 3 continuous days, followed by the administration of a single dose (50 mg/kg) on day 4 before administration of naloxone (19,20).…”

Section: Induction Of Tolerance and Dependencementioning

confidence: 99%

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Assessment of Toxicity and Biological Effects of α-Pinene on Morphine Tolerance and Dependence in Mice

Nasrin Hajiabadi,

Abbasi Maleki,

Mousavi

et al. 2023

Jundishapur J Nat Pharm Prod

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Background: Research shows that α-Pinene interacts with the opioidergic system. Objectives: This study aims to examine the toxicity and the effects of α-Pinene on morphine tolerance and dependence in mice. Methods: Guidelines No. 423 and No. 407 were used to investigate acute and sub-chronic toxicity, respectively. For sub-chronic toxicity analysis, the animals were sacrificed on day 28, and blood and tissue samples were collected. After inducing morphine tolerance or dependence, in both phases, animals received i.p. vehicle, diazepam (5 mg/kg), and α-Pinene (3.125, 6.25, and 12.5 mg/kg). Withdrawal signs were recorded for 30 minutes. Results: Only the acute dose of α-Pinene showed mortality in animals, but mild lesions were seen in the brain, liver, and kidneys in the mice receiving its subchronic dose. Moreover, ALT, AST, ALP, and TG levels increased (P < 0.05) in female mice. Besides, 6.25 and 12.5 mg/kg (P < 0.001) of α-Pinene and only its high dose (12.5 mg/kg) (P < 0.001) reduced the number of jumps in the tolerance and dependence phases, respectively. Diarrhea (P < 0.001), writhing (P < 0.001), rearing, and climbing (P < 0.05 and P < 0.001, respectively) behaviors decreased in the tolerance phase, and grooming, climbing, and teeth chattering declined in the dependence phase (P < 0.001). Conclusions: The LD50 of α-Pinene was lower than 2000 mg/kg, but its subchronic dose caused mild tissue toxicities and biochemical changes. Moreover, α-Pinene decreased morphine tolerance and dependence and possibly was useful for the treatment of opioid dependence after complimentary trials.

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The effect of hydroalcoholic extract of Urtica dioica on morphine withdrawal signs in male mice

Abstract: The present study findings showed that Urtica dioica extract could decrease the morphine withdrawal signs and might be beneficial in addicted patients.

Cited by 2 publications

References 25 publications

In Vitro Scolicidal Effect of Urtica dioica and Pyrus boissieriana Extracts Against Protoscoleces of Echinococcus granulosus

In Vitro Scolicidal Effect of Urtica dioica and Pyrus boissieriana Extracts Against Protoscoleces of Echinococcus granulosus

Assessment of Toxicity and Biological Effects of α-Pinene on Morphine Tolerance and Dependence in Mice

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