The effect of hyaluronan on the motility of skin dermal fibroblasts in nanofibrous scaffolds

Qian, Yuna; Li, Linhao; Jiang, Chao; Xu, Weiqing; Lv, Yonggang; Zhong, Li; Cai, Kaiyong; Yang, Li

doi:10.1016/j.ijbiomac.2015.04.059

Cited by 33 publications

(19 citation statements)

References 44 publications

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“…produced PCL/HA nanofibrous scaffolds, where PCL and HA offered, respectively, mechanical stability and biochemical cues. 88 Enhanced fibroblasts infiltration and motility were observed both in vitro and in vivo for PCL/HA membranes if compared with PCL ones. This was possibly due to the interaction between HA and cell surface receptors.…”

Section: Collagen and Gelatinementioning

confidence: 94%

Electrospinning of natural polymers for advanced wound care: towards responsive and adaptive dressings

2016

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Section: Collagen and Gelatinementioning

confidence: 94%

Electrospinning of natural polymers for advanced wound care: towards responsive and adaptive dressings

2016

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“…The solution of pure hyaluronic acid [132] or with relatively small amount of carrier PEO was successfully spun into nanofibrous material by air-assisted electrospinning technology, that is, electroblowing [133]. For creation of nanofibrous scaffolds, hyaluronic acid was also used in combination with PCL [134], PLA [135] or gelatin, chondroitin sulfate and sericin [86].…”

Section: Nature-derived Nanofibers Degradable In the Human Tissuesmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

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“…The major characteristic of hypertrophic scars and keloids is a metabolism disorder of collagen-based ECM proteins [4,34]. The effect of nanofibers on fibrosis-associated genes [35], such as collagen I, collagen III and fibronectin, are important for understanding the cellular responses to scaffolds and particularly the effects on scar formation [8]. The expression of collagen I, collagen III and fibronectin in the cells cultured on nanofiber scaffolds were downregulated compared to TCP control ( figure 2(B)).…”

Section: Pcl/gelatin Fibers Promoting Cell Proliferation and Decreasimentioning

confidence: 99%

Nanofiber scaffolds attenuate collagen synthesis of human dermal fibroblasts through TGF-β1/TSG-6 pathway*

et al. 2019

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Nanofiber scaffolds are promising patches for skin tissue regeneration as they provide favorable environment for the adhesion, infiltration and proliferation of skin dermal fibroblasts. However, the effects of nanofiber scaffolds on scar formation remain to be elucidated. The aim of this study was to find out the relationship between nanofiber scaffolds and scar formation, along with the underlying mechanism. We found that polycaprolactone (PCL)/gelatin nanofiber scaffolds attenuated the mRNA expression of fibrosis-associated genes in fibroblasts, including collagen I (collagen type I alpha 1), collagen III (collagen type III alpha 1) and fibronectin. Specifically thicker scaffolds displayed stronger fibrosis inhibitory effect than thin scaffolds. The mechanism relied on TGF-β1/TSG-6 pathway, and overexpression of TSG-6 impaired the anti-fibrosis effect of nanofiber scaffolds, which decreased TGF-β1 expression with thickness-dependency. Moreover, in vivo study demonstrated that nanofiber scaffolds remarkably accelerated the wound healing process by reducing the ratios of collagen I/ collagen III and TGF-β1, eventually decreased the deposition of collagens. Taken together, our results suggested that the attenuation of fibrosis by PCL/gelatin nanofiber scaffolds was TGF-β1-dependent and through TGF-β1/TSG-6 pathway. Nanofiber scaffold of appropriate thickness would accelerate skin wound healing, stimulate re-epithelialization and form cutaneous skin appendages in skin trauma. Thus, PCL/gelatin nanofiber scaffolds could be adopted for scar-free skin wound healing and skin cosmetics applications.

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The effect of hyaluronan on the motility of skin dermal fibroblasts in nanofibrous scaffolds

Cited by 33 publications

References 44 publications

Electrospinning of natural polymers for advanced wound care: towards responsive and adaptive dressings

Electrospinning of natural polymers for advanced wound care: towards responsive and adaptive dressings

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Nanofiber scaffolds attenuate collagen synthesis of human dermal fibroblasts through TGF-β1/TSG-6 pathway*

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