The effect of humic-fatty acid preparation on selected haematological and biochemical serum parameters of growing rabbits

Miśta, Dorota; Rząsa, Anna; Wincewicz, E.; Zawadzki, Wojciech; Dobrzański, Zbigniew; Szmanko, T.; Gelles, A

doi:10.2478/v10181-012-0061-z

Cited by 11 publications

(9 citation statements)

References 4 publications

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“…Serum mineral levels (Ca, P, Mg and Fe) were not affected by ZnO + HNa treatments. HS appear to have metal-chelating effects and were reported to reduce levels of serum minerals in broilers (Rath et al 2006;Celik et al 2008;Samudovska and Demeterova 2010) and rabbits (Mista et al 2012), although this effect was not observed with HNa in weaned piglets (Trckova et al 2015). Significantly higher Zn levels in the serum of the ZnO 2.5 group reflected the higher Zn dietary intake in the group.…”

Section: Clinical Biochemical and Haematological Indicators Of Healtmentioning

confidence: 82%

“…The increase of HCT and HGB was also observed when HA was fed to broilers and rabbits (Ipek et al 2008;Mista et al 2012), although no data have as yet been reported from pigs. In our previous study, we observed non-significantly higher HCT and HGB when ZnO and HNa were fed to piglets challenged by E. coli (Trckova et al 2015).…”

mentioning

confidence: 89%

“…This is in accordance with Zhang et al (2014), who reported a dosedependent increase in TGC with supplementation of dietary Zn. On the other hand, HS were 191.5 ± 0.03 286.8 ± 0.03 249.5 ± 0.04 271.9 ± 0.04 ** ** ** ** ns ns ns Feed intake (g/pig/day) 453.5 ± 287.5 500.9 ± 327.5 495.8 ± 324.6 492.4 ± 304.8 ns described to decrease serum TGC and cholesterol in animals (Samudovska and Demeterova 2010;Mista et al 2012;Ozturk et al 2012). In our study, partial substitution of ZnO by HNa in feed resulted in significantly lower serum TGC in comparison with ZnO 2.5, but this was not observed when HNa was supplemented in water.…”

Section: Clinical Biochemical and Haematological Indicators Of Healtmentioning

confidence: 99%

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Effects of sodium humate and zinc oxide used in prophylaxis of post-weaning diarrhoea on the health, oxidative stress status and fatty acid profile in weaned piglets

Trčková¹,

Lorencova²,

Babák³

et al. 2017

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to investigate the effects of sodium humate (HNa), applied to feed or drinking water as a partial substitution for pharmacological doses of ZnO, on clinical, biochemical and haematological indicators of health status, oxidative stress and the serum fatty acid (FA) profile in weaned piglets. Weaned piglets (32) were allocated into four groups: Control = basal diet containing 110 mg ZnO/kg; ZnO 2.5 = pharmacological dose of ZnO (2.5 g ZnO/kg); ZnO 1.7 + HNa (f ) = 1.7 g ZnO and 20.0 g HNa/kg; ZnO 1.7 + HNa (w) = 1.7 g ZnO/kg and drinking water with 0.2% HNa. All ZnO treatments resulted in good performance and clinical health of piglets in contrast to Control, which contained three diarrhoeic piglets. Increased triacylglycerols in the ZnO 2.5 and ZnO 1.7 + HNa (w) groups in comparison with Control suggested increased energy metabolism after treatments. Neither total cholesterol, nor HDL and LDL were affected by treatments. Piglets treated with ZnO and HNa had (ZnO 1.7 + HNa (w)) or tended to have (ZnO 1.7 + HNa (f )) lower urea in serum. Significantly (in ZnO 2.5 and ZnO 1.7 + HNa (f )) or non-significantly (ZnO 1.7 + HNa (w)) higher haematocrit and haemoglobin levels were detected in the blood of treated piglets. Long-term ZnO 2.5 treatment significantly increased serum 8-iso-PGF 2α , the most reliable biomarker of oxidative stress. Partial substitution of ZnO by HNa positively affected the oxidative status of piglets as evidenced by significant (ZnO 1.7 + HNa (w)) or non-significant (ZnO 1.7 + HNa (f )) declines in serum 8-iso-PGF 2α . ZnO 2.5 treatment significantly decreased saturated (SFA), monounsaturated (MUFA) and n-3 polyunsaturated FA (PUFA) and increased PUFA n-6 and n-6/n-3 ratios in serum compared to Control. The synthesis of some physiologically significant long-chain PUFA (LC-PUFA), namely n-6 dihomo-γ-linolenic acid and n-3 eicosapentaenoic, docosapentaenoic was negatively affected by ZnO 2.5 treatment. Both ZnO 1.7 + HNa (f )/(w) treatments significantly increased the proportion of SFA and PUFA n-3 and decreased PUFA n-6 and n-6/n-3 ratios in comparison with ZnO 2.5 and the effect was more considerable in ZnO 1.7 + HNa (w). Most individual PUFA n-3 and n-6 as well as the n-6/n-3 ratio in both treatments were similar to Control. The results indicate that the partial substitution of ZnO by HNa can benefit performance and health of weaned piglets to a similar extent as a high pharmacological dose of ZnO, whereas it can decrease the oxidative stress induced by prolonged over-supplementation of ZnO. Additionally, such a treatment can eliminate the unfavourable effect of high ZnO doses on the n-6/n-3 ratio and the proportion of some physiologically significant LC-PUFA in serum. Generally, it can be concluded that the effects of feed and water HNa supplementation are similar, but are more pronounced when HNa is applied to drinking water.Keywords: humic substances; pig; lipid metabolism; eicosanoids; serum biochemistry; haematology; performance; GC/MS; LC/MS-MS List of ab...

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Section: Clinical Biochemical and Haematological Indicators Of Healtmentioning

confidence: 82%

mentioning

confidence: 89%

Section: Clinical Biochemical and Haematological Indicators Of Healtmentioning

confidence: 99%

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Effects of sodium humate and zinc oxide used in prophylaxis of post-weaning diarrhoea on the health, oxidative stress status and fatty acid profile in weaned piglets

Trčková¹,

Lorencova²,

Babák³

et al. 2017

Vet. Med. - Czech

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“…HS were reported to reduce serum minerals (P, Ca, Mg and Fe) in broilers (Rath et al 2006;Celik et al 2008;Demeterova et al 2009;Samudovska and Demeterova 2010), and rabbits (Mista et al 2012). A potential metal-chelating effect might be exerted by many reactive functional groups located in the structures of HS (Rath et al 2006).…”

Section: Haematological and Serum Biochemical Profilementioning

confidence: 99%

“…HS were described to decrease serum triacylglycerols and cholesterol levels in animals (Herzig et al, 2009;Samudovska and Demeterova 2010;Mista et al 2012;Ozturk et al 2012). After three weeks of HNa dietary treatment we observed only slightly (non-sig- 1 C = control with no supplementation; ZnO2.5 = supplemented with high prophylactic dose of ZnO (2.5 g/kg diet); HNa + ZnO1.7, HNa + ZnO1.5, HNa + ZnO1.0 = supplemented with HNa (20.0 g/kg diet) and 1.7, 1.5 and 1.0 g ZnO/kg diet, respectively The individual pig served as the experimental unit.…”

Section: Haematological and Serum Biochemical Profilementioning

confidence: 99%

Prophylaxis of post-weaning diarrhoea in piglets by zinc oxide and sodium humate

Trčková¹,

Lorencova²,

Hazova³

et al. 2015

Vet. Med. - Czech

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ABSTRACT:The high prophylactic doses of ZnO commonly used to control post-weaning diarrhoea (PWD) in piglets have become an environmental problem. The possibility of partial replacement of ZnO by sodium humate (HNa) in PWD treatment was investigated in this study. Weaned piglets (32) were challenged with two enterotoxigenic E. coli strains (ETEC/O149/F4/LT and ETEC/O147/F18/LT) and allocated into four treatments maintained for three weeks: C (no supplementation); ZnO2.5 (2.5 g ZnO/kg diet); HNa + ZnO1.0; HNa + ZnO1.5; HNa + ZnO1.7 (20 g HNa and 1.0, 1.5 and 1.7 g ZnO/kg diet, respectively). The effects on incidence, severity and duration of diarrhoea, faecal shedding of total E. coli and both ETEC strains, growth rate and selected blood parameters were investigated. In contrast with ZnO2.5 and HNa + ZnO1.7, high daily diarrhoea scores, incidence and duration and mortality due to severe dehydration were seen in C, HNa + ZnO1.0 and HNa + ZnO1.5 groups. The administration of ZnO and HNa did not affect the faecal shedding of the challenged ETEC strains for eght days, even in clinically healthy piglets in ZnO2.5 and HNa + ZnO1.7 groups. Signs of growth depression were found in C; HNa + ZnO1.0 and HNa + ZnO1.5 groups during the first week. No difference in growth performance was observed in ZnO2.5 and HNa + ZnO1.7 piglets. Most of the selected biochemical and haematological parameters did not differ significantly among the treatments. However, a significantly higher serum Zn as a result of high dietary ZnO intake in the ZnO2.5 group compared to the control and HNa groups was detected. Significantly lower serum P in ZnO2.5; HNa + ZnO1.7 and HNa + ZnO1.0 groups compared to the control group was most likely induced by the increased Zn in serum. The results indicate the possibility of reducing the high pharmacological levels of ZnO in the prophylaxis of PWD through partial replacement with HNa. Such a treatment maintains the favourable prophylactic effect while lowering the Zn content in faeces.Keywords: humic; enterotoxigenic E. coli; faecal shedding; growth performance; blood chemistry List of abbreviations: ALP = alkaline phosphatase, ALT = alanine transaminase, ANOVA = analyses of variance, AST = aspartate aminotransferase, BWG = body weight gains, CFU = colony forming units, DDS = daily diarrhoea score, ETEC = enterotoxigenic Escherichia coli, FCR = feed conversion ratio, FI = feed intake, HNa = sodium humate, HS = humic substances, PWD = post-weaning diarrhoea

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Effects of Humic Acids in Chronic Lead Poisoning

Vašková

Krempaská

Žatko

et al. 2018

Biol Trace Elem Res

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Chronic exposure to lead causes disruption to energy production mechanisms and tissue damage, in particular through its binding to thiol groups and competition for zinc binding sites. We investigated the possibility of preventing the consequences of chronic lead poisoning by administration of three different doses of humic acids (HAs) into feed with the aim of establishing an effective HA dose. During the 10-week experiment, a sub-lethal dose of lead acetate was given to rats during the first 5 weeks, with continuous administration of HA over 10 weeks. Measurements were taken to determine the content of the metals Pb, Mn, Cu, Fe and Zn; the metalloid Se; and selected antioxidant markers in the heart, liver, kidney and plasma after the first, fifth and tenth weeks of experiment. The administration of lead and HAs clearly affects the redistribution of the elements and the activity of the antioxidant enzymes. This fact was particularly highlighted in the lead-only group as, within the experiment, significantly higher Pb concentrations were found only in the plasma of this group. However, in the group with 1% HA administered with lead, we observed a rise in Zn concentrations in the organs and the deposition of Fe into the liver. Decreased glutathione reductase activity in the plasma and balanced reduced glutathione concentrations indicated sufficient efficiency of redox reactions. SOD activities were among those affected most strongly, with only the 1% HA group showing no effect on heavy metal redistribution as a result of HA administration.

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The effect of humic-fatty acid preparation on selected haematological and biochemical serum parameters of growing rabbits

Cited by 11 publications

References 4 publications

Effects of sodium humate and zinc oxide used in prophylaxis of post-weaning diarrhoea on the health, oxidative stress status and fatty acid profile in weaned piglets

Effects of sodium humate and zinc oxide used in prophylaxis of post-weaning diarrhoea on the health, oxidative stress status and fatty acid profile in weaned piglets

Prophylaxis of post-weaning diarrhoea in piglets by zinc oxide and sodium humate

Effects of Humic Acids in Chronic Lead Poisoning

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