The effect of histamine on kidney by fasting in rats

Abstract

Abstract:Background: The aim of this study was to investigate ultrastructural and apoptotic changes occurring in the kidneys in fasting individuals and to examine the effects of histamine treatment at the electron-microscopic and immunohistochemical levels. Methods: Eighteen adult Wistar male rats were randomly divided into three groups (n=6 for each). Control group (1), fasting group (12 h) (2), and fasting+histamine injection (0.5 mg/kg) (3) group. Expression of caspase-3 and caspase-9 was determined in the tissue sections using immunohistochemical techniques. Quantitative data were obtained using H-SCORE, and statistical evaluations were then performed. The ultrastructure of the kidney tissues was examined using transmission electron microscopy. Results: Weak caspase-3 and caspase-9 expression was observed in the renal tubules and glomeruli in the control group, while immunoreactivity was more intense in the fasting group (p < 0.05). In the fasting+histamine group, caspase-3 and caspase-9 immunostaining was signifi cantly positive in both renal tubules and glomeruli (p <0.05). At electron microscopic evaluation, degenerative changes were seen in the glomeruli of the fasting group, as well as partial vacuolization and disruption at the basal foldings in the tubular epithelial cells. In the fasting+histamine group, in addition to signifi cant dilatation of all glomerular capillaries, there were degenerative changes in all tubular and canalicular epithelial cells in the proximal tubules. Conclusions: Fasting, an important metabolic stress factor, accompanied by histamine treatment may cause signifi cant disruptions in the kidneys, particularly in the glomerular capillaries and proximal and distal tubules (Tab. 1, Fig. 2 Acknowledgements: We confi rm that we have read the Journal's position on issues involved in ethical publication and affi rm that this report is consistent with those guidelines.

Ultrastructural analysis of different human mesenchymal stem cells after in vitro expansion: a technical review

“… 1 Cell pellet was fixated at the room temperature for 3 h in 2.5% glutaraldehyde solution in phosphate buffer (pH 7.2). 18 The crucial part was maintaining a steady pH by adding sodium hydrogen phosphate with water or potassium dihydrogen phosphate, when needed. Then, cells with fixative were rinsed with the cooled phosphate buffer with 10% saccharose, 3 times for 30 min and left sealed for one night in the refrigerator.…”

Histamine in the kidneys: what is its role in renal pathophysiology?

“…Nonetheless, histamine, given i.p. at 0.5 mg·kg −1 , induced loss of foot processes in fasting rats (Gurgen, Erdogan, & Take‐Kaplanoglu, 2013). These glomerular changes correlate with the filtration capacity of the kidneys and affect creatinine and urea clearance.…”

“…The role of histamine in renal disease can be extrapolated from the analysis above.‐Moreover, the relative contribution of each histamine receptor reflects their distribution, with histamine triggering both degenerative glomerular and tubular changes (Bjering, 1937; Gurgen et al, 2013), via different histamine receptor pathways.…”

Histamine H 4 receptor antagonism prevents the progression of diabetic nephropathy in male DBA2/J mice

“…Actually, the first evidence for a possible role of histamine in diabetic nephropathy was provided in the 1960s when, concurrent with an increased tissue HDC activity, an increased level of this amine was observed in the kidney of diabetic animals [12,13]. In particular, histamine has already been demonstrated to increase salt and water excretion [14][15][16] and renin release [17], and to decrease the ultrafiltration coefficient [18]. These effects were classically attributed to the well-known vasoactive properties of the amine.…”