The effect of high humidity culture conditions over embryo development: a continuous embryo monitoring assessment

Albert, Carmela; Gonzalez, Naiara; Marcos, Julián; Alegre, Lucía; Ruiz, B. Aparicio; Santos, J.M. De los; Meseguer, M.

doi:10.1016/j.rbmo.2018.04.029

“…Fawzy et al (2017a) reported that introducing humidity into a normally dry incubator resulted in improved embryo development and ongoing pregnancy rates. Albert et al (2018) used time-lapse imaging to evaluate the effect of removing humidification from a normally humidified incubator on embryo development, and also reported better outcomes with humidification. It must also be noted that 'humid' versus 'dry' is not a binary condition, and aspects of relative volumes and both surface and interface areas are likely to influence the precise relationship between a humidified incubator and the actual culture environment experienced by the embryos, and vice versa.…”

Section: Humidity In Culturementioning

confidence: 99%

‘There is only one thing that is truly important in an IVF laboratory: everything’ Cairo Consensus Guidelines on IVF Culture Conditions

Group¹

2020

Reproductive BioMedicine Online

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Cairo 2018 Consensus Group* KEY MESSAGE This report presents outcomes from an international expert meeting to establish consensus guidelines on IVF culture. Topics reviewed were: embryo culture; temperature; humidity; gas control, pH; workstations; incubators; micromanipulation; handling and assessment; stasis, composition, supplementation, type of culture and storage; equipment and monitoring. More than 50 consensus guideline points were established.

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“…For example, the DC mode was associated with quality decline in the embryo at the cleavage stage and a lower rate of blastocyst formation compared with the HC mode, resulting in a decreased implantation and ongoing pregnancy rates [7]. In addition, there was strong evidence that culture conditions with high humidity atmosphere promoted embryo development and reproductive outcome [14]. In the Cairo consensus guidelines on IVF culture conditions, the DC and HC contributed to satisfactory outcome in clinical settings, but the HC was proved to be more suitable for IVF-ET [3].…”

Section: Discussionmentioning

confidence: 99%

“…It provides a saturated humidity in the culture environment for embryo by means of water vaporization in an active manner or humidi cation based on disposable humidi cation ask. On this basis, the osmotic pressure of culture medium is stable [1,2], contributing to the culture of zygote and embryo. Therefore, it is recommended as the major method for embryo culture [3].…”

Section: Introductionmentioning

confidence: 99%

Efficiency comparison between dry and humid cultures for clinical outcome of the in vitro fertilization-embryo transfer

Xu

¹

,

Zhang

²

,

Zhang

³

et al. 2021

Preprint

0

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Background: In this study, we compared the in vitro embryo development, embryo transfer outcome and the offspring outcome in the in vitro fertilization-embryo transfer (IVF-ET) between dry culture (DC) and humid culture (HC). Methods: Our study was divided into two parts. Firstly, we determined the fertilization rate, cleavage rate and high-quality embryo rate from 21 cycles in the DC group (N=262 oocytes) and HC group (N=263 oocytes). Secondly, we determined the embryo transfer outcome and the offspring outcome in DC group (N=184 cycles) and HC group (N=136 cycles). Results: Compared with the HC group, significant increase was observed in the high-quality embryo rate (66.1.2% vs. 55.3%, p=0.037) and implantation rate (49.8% vs. 40.6%, p=0.027) in the DC group. No statistical differences were observed in the pregnant outcome and birth defect of the offspring (p>0.05). Compared with HC, DC was associated with a higher high-quality embryo rate and a higher implantation rate after embryo transfer. Conclusions: No statistical differences were noticed in the offspring conditions between the two culture modes. Taken together, DC may serve as a promising method for IVF-ET.

show abstract

“…For example, the DC mode was associated with quality decline in the embryo at the cleavage stage and a lower rate of blastocyst formation compared with the HC mode, resulting in a decreased implantation and ongoing pregnancy rates [7]. In addition, there was strong evidence that culture conditions with high humidity atmosphere promoted embryo development and reproductive outcome [14]. In the Cairo consensus guidelines on IVF culture conditions, the DC and HC contributed to satisfactory outcome in clinical settings, but the HC was proved to be more suitable for IVF-ET [3].…”

Section: Discussionmentioning

confidence: 99%

“…It provides a saturated humidity in the culture environment for embryo by means of water vaporization in an active manner or humidi cation based on disposable humidi cation ask. On this basis, the osmotic pressure of culture medium is stable [1,2], contributing to the culture of zygote and embryo. Therefore, it is recommended as the major method for embryo culture [3].…”

Section: Introductionmentioning

confidence: 99%

Efficiency Comparison Between Dry And Humid Cultures For Clinical Outcome of The In Vitro Fertilization-Embryo Transfer

Xu

¹

,

Zhang

²

,

Zhang

³

et al. 2021

Preprint

1

0

View full text Add to dashboard Cite

Background: In this study, we compared the in vitro embryo development, embryo transfer outcome and the offspring outcome in the in vitro fertilization-embryo transfer (IVF-ET) between dry culture (DC) and humid culture (HC). Methods: Our study was divided into two parts. Firstly, we determined the fertilization rate, cleavage rate and high-quality embryo rate from 21 cycles in the DC group (N=262 oocytes) and HC group (N=263 oocytes). Secondly, we determined the embryo transfer outcome and the offspring outcome in DC group (N=184 cycles) and HC group (N=136 cycles). Results: Compared with the HC group, significant increase was observed in the high-quality embryo rate (66.1.2% vs. 55.3%, p=0.037) and implantation rate (49.8% vs. 40.6%, p=0.027) in the DC group. No statistical differences were observed in the pregnant outcome and birth defect of the offspring (p>0.05). Compared with HC, DC was associated with a higher high-quality embryo rate and a higher implantation rate after embryo transfer. Conclusions: No statistical differences were noticed in the offspring conditions between the two culture modes. Taken together, DC may serve as a promising method for IVF-ET.

show abstract

The effect of high humidity culture conditions over embryo development: a continuous embryo monitoring assessment

Cited by 3 publications

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‘There is only one thing that is truly important in an IVF laboratory: everything’ Cairo Consensus Guidelines on IVF Culture Conditions

‘There is only one thing that is truly important in an IVF laboratory: everything’ Cairo Consensus Guidelines on IVF Culture Conditions

Efficiency comparison between dry and humid cultures for clinical outcome of the in vitro fertilization-embryo transfer

Efficiency Comparison Between Dry And Humid Cultures For Clinical Outcome of The In Vitro Fertilization-Embryo Transfer

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