The effect of high concentration isopropanol on the growth of a solvent-tolerant strain of Chlorella vulgaris

McEvoy, Eileen; Wright, Phillip C.; Bustard, Mark Thomas

doi:10.1016/j.enzmictec.2004.01.012

Cited by 13 publications

(8 citation statements)

References 21 publications

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“…Isopropanol was quickly transformed by the biomass to acetone, which was then biodegraded slowly by the biomass. Acetone was not reported as intermediate product in the isopropanol biofiltration (Chang and Lu, 2003;Liu et al, 2005), but it was reported as a metabolic product in isopropanol biodegradation in several suspension cultures (Bustard et al, 2002;Leethochawalit et al, 2004;McEvoy et al, 2004). It was also reported that isopropanol was biodegraded via the acetone metabolic pathway (Radianingtyas and Wright, 2003).…”

Section: Single Substrate Biofiltrationmentioning

confidence: 90%

CO2 Response to Doses of Organic Solvents Biodegraded in a Batch Biofilter

Fang¹,

Govind

2006

Water Air Soil Pollut

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Carbon dioxide served as a proxy for biodegradation in the biofiltration of multiple organic solvents in a batch biofilter. The solvents were injected into the batch biofilter system and were biodegraded with the biomass immobilized on a column of fibrous media; the immobilized biomass was acclimated to ethanol in a continuous biofilter. Time profiles of CO 2 concentration were obtained for substrate utilization and for biomass endogenous respiration. The CO 2 and substrate concentration profiles provided insight for the biodegradation of multiple substrates in a biofilter. Experimental results indicate that the biomass utilized the solvents simultaneously but at different rates. The utilization rates were in the order: acetaldehyde > ethanol > propanol > isopropanol, acetone > methanol > butanol. Isopropanol was transformed to acetone, which was then utilized and converted to CO 2 , and propanol was also utilized through the formation of an intermediate. The biodegradation of ethanol was inhibited by acetaldehyde. Total CO 2 production was contributed by the endogenous respiration of the biomass as well as by the biodegradation of all the substrates present to the biomass, and the total CO 2 rate might not reach its maximum potential.

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Section: Single Substrate Biofiltrationmentioning

confidence: 90%

CO2 Response to Doses of Organic Solvents Biodegraded in a Batch Biofilter

Fang¹,

Govind

2006

Water Air Soil Pollut

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“…Microbes have grown at different rates in these organic solvents and the growth rate followed the order: ethanol > isopropanol > methanol > acetone > toluene > acetonitrile. Biokinetic parameters obtained in this study are compared with those reported earlier in literature [21][22][23][24][25]. The maximum specific growth rates of various microbial cultures for isopropanol biodegradation reported in literature varied from 0.0017 to 0.2/h.…”

Section: Single-substrate Biodegradation Studiesmentioning

confidence: 95%

Biodegradation of Chlorinated and Non-chlorinated VOCs from Pharmaceutical Industries

Balasubramanian

Philip

Bhallamudi

2010

Appl Biochem Biotechnol

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Biodegradation studies were conducted for major organic solvents such as methanol, ethanol, isopropanol, acetone, acetonitrile, toluene, chloroform, and carbon tetrachloride commonly used in pharmaceutical industries. Various microbial isolates were enriched and screened for their biodegradation potential. An aerobic mixed culture that had been previously enriched for biodegradation of mixed pesticides was found to be the most effective. All the organic solvents except chloroform and carbon tetrachloride were consumed as primary substrates by this mixed culture. Biodegradation rates of methanol, ethanol, isopropanol, acetone, acetonitrile, and toluene were measured individually in batch systems. Haldane model was found to best fit the kinetics of biodegradation. Biokinetic parameters estimated from single-substrate experiments were utilized to simulate the kinetics of biodegradation of mixture of substrates. Among the various models available for simulating the kinetics of biodegradation of multi-substrate systems, competitive inhibition model performed the best. Performance of the models was evaluated statistically using the dimensionless modified coefficient of efficiency (E). This model was used for simulating the kinetics of biodegradation in binary, ternary, and quaternary substrate systems. This study also reports batch experiments on co-metabolic biodegradation of chloroform, with acetone and toluene as primary substrates. The Haldane model, modified for inhibition due to chloroform, could satisfactorily predict the biodegradation of primary substrate, chloroform, and the microbial growth.

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“…Bustard reported on the treatment of IPA concentration up to 24 g=L by an aerobic solvent-tolerant population. [8] To date, only a few IPA-degrading microbial strains, belonging to a diverse genera, have been isolated all over the world: Bacillus, [9] Chlorella, [1] and Sphingobacterium. [5] In this study, we report for the first time utilization by Paracoccus denitrificans of high concentrations of IPA as sole carbon source under aerobic culture conditions, focusing on specific growth rate, IPA biodegradation, and acetone generation.…”

Section: Introductionmentioning

confidence: 99%

“…High concentrations of IPA are toxic to most microorganisms due to its high oxygen demand and membrane-disruptive characteristics. [1] Because of its deleterious effects, discharging IPA into the environment without any treatment will cause serious damage to the whole ecosystem. Hitherto, the apparent consumption of IPA in China has been 200 kton=year.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Isopropanol by a Solvent-TolerantParacoccus denitrificansStrain

Geng

Deng

Chen

et al. 2014

Preparative Biochemistry and Biotechnology

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The biodegradation of high concentration isopropanol (2-propanol, IPA) at 16 g/L was investigated by a solvent-tolerant strain of bacteria identified as Paracoccus denitrificans for the first time by 16S rDNA gene sequencing. The strain P. denitrificans GH3 was able to utilize the high concentration of IPA as the sole carbon source within a minimal salts medium with a cell density of 1.5×10(8) cells/mL. The optimal conditions were found as follows: initial pH 7.0, incubation temperature 30°C, with IPA concentration 8 g/L. Under the optimal conditions, strain GH3 utilized 90.3% of IPA in 7 days. Acetone, the major intermediate of aerobic IPA biodegradation, was also monitored as an indicator of microbial IPA utilization. Both IPA and acetone were completely removed from the medium following 216 hr and 240 hr, respectively. The growth of strain GH3 on IPA as a sole carbon and energy source was well described by the Andrews model with a maximum growth rate (μmax)=0.0277/hr, a saturation constant (KS)=0.7333 g/L, and an inhibition concentration (Ki)=8.9887 g/L. Paracoccus denitrificans GH3 is considered to be well used in degrading IPA in wastewater.

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The effect of high concentration isopropanol on the growth of a solvent-tolerant strain of Chlorella vulgaris

Cited by 13 publications

References 21 publications

CO2 Response to Doses of Organic Solvents Biodegraded in a Batch Biofilter

CO2 Response to Doses of Organic Solvents Biodegraded in a Batch Biofilter

Biodegradation of Chlorinated and Non-chlorinated VOCs from Pharmaceutical Industries

Biodegradation of Isopropanol by a Solvent-TolerantParacoccus denitrificansStrain

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