The effect of hemostatic dressing prototypes for the uniformed services on selected blood coagulation parameters in pigs

Adamiak, Z.; Krystkiewicz, Wioletta; Pomianowski, A.; Bukowiecka, Danuta; Zubrzycki, Waldemar; Jałyński, M.; Holak, Piotr; Głodek, Joanna; Jastrzębski, Paweł

doi:10.1186/s13028-017-0297-9

Cited by 4 publications

(7 citation statements)

References 7 publications

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“…PT, 31 53 58 aPTT, 31 53 58 ACT, 52 fibrinogen, 31 53 56 58 D-dimer, 31 53 ROTEM, 31 (ATIII, TAT), 53 TEG, 54 ROTEM 56…”

Section: Resultsmentioning

confidence: 99%

The Use of Large Animal Models in Trauma and Bleeding Studies

Rayatdoost,

Grottke

2023

Hamostaseologie

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Background Major trauma often results in significant bleeding and coagulopathy, posing a substantial clinical burden. To understand the underlying pathophysiology and to refine clinical strategies to overcome coagulopathy, preclinical large animal models are often used. This review scrutinizes the clinical relevance of large animal models in hemostasis research, emphasizing challenges in translating findings into clinical therapies. Methods We conducted a thorough search of PubMed and EMBASE databases from January 1, 2010, to December 31, 2022. We used specific keywords and inclusion/exclusion criteria centered on large animal models. Results Our review analyzed 84 pertinent articles, including four animal species: pigs, sheep, dogs, and nonhuman primates (NHPs). Eighty-five percent of the studies predominantly utilized porcine models. Meanwhile, sheep and dogs were less represented, making up only 2.5% of the total studies. Models with NHP were 10%. The most frequently used trauma models involved a combination of liver injury and femur fractures (eight studies), arterial hemorrhage (seven studies), and a combination of hemodilution and liver injury (seven studies). A wide array of coagulation parameters were employed to assess the efficacy of interventions in hemostasis and bleeding control. Conclusions Recognizing the diverse strengths and weaknesses of large animal models is critical for trauma and hemorrhage research. Each model is unique and should be chosen based on how well it aligns with the specific scientific objectives of the study. By strategically considering each model's advantages and limitations, we can enhance our understanding of trauma and hemorrhage pathophysiology and further advance the development of effective treatments.

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“…PT, 31 53 58 aPTT, 31 53 58 ACT, 52 fibrinogen, 31 53 56 58 D-dimer, 31 53 ROTEM, 31 (ATIII, TAT), 53 TEG, 54 ROTEM 56…”

Section: Resultsmentioning

confidence: 99%

The Use of Large Animal Models in Trauma and Bleeding Studies

Rayatdoost,

Grottke

2023

Hamostaseologie

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“…The applied pro-coagulant treatment enhanced the physiological effects of thrombin and activated intrinsic inhibitors, the main one being ATIII. Antithrombin III inactivates thrombin by forming biologically inactive TAT complexes with this enzyme (10). Thrombin-antithrombin complexes have an estimated half-life of 15 min (14).…”

Section: Discussionmentioning

confidence: 99%

“…In both groups I and II, platelet counts increased after 7 days following injury. Thrombocytes release adrenalin, noradrenalin, serotonin and thromboxane which promote vasoconstriction and minimise blood loss after injury (10). Aggregating thrombocytes form platelet plugs which prevent bleeding (6).…”

Section: Discussionmentioning

confidence: 99%

“…Both types of dressings in groups I and II were coated by chitosan (ChitoClear hqg 95 from Primex ehf Iceland) and sodium alginate (Protanal LF10/60 FT) at a concentration of 18.0 g/m 2 for both. Haemostatic dressings used in a previous study (10) were modified here. Group III (C) animals (n=8) were treated with a type of non-haemostatic dressing and were used as a negative control group.…”

Section: Methodsmentioning

confidence: 99%

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The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System

Adamiak

Borys²,

Krystkiewicz

et al. 2019

In Vivo

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Background/Aim: Coagulopathy can develop when hemostatic dressings are used to stop massive bleeding, even in patients without prior history of clotting disorders. The selection of procoagulants, which effectively control bleeding and prevent disseminated intravascular coagulation (DIC) and thrombosis, is a significant challenge. The aim of this study was to evaluate the effect of two prototypes of haemostatic dressing in the porcine haemostatic system. Materials and Methods: The total number of animals used in our experiments was 24. Group I: pigs were treated with the developed prototype of sponge dressing, made of Na-Ca chitosan/algal composite of microfibers and nanofibers. Group II: animals were treated with a seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite of microfibers and nanofibers. Group III: animals were treated with non-hemostatic dressing and this group was the control. Blood was sampled five times to determine changes in the coagulation and fibrinolytic profiles: before injury: i) at 1 h, ii) at 24 h, iii) at 7, and iv) at 14 days following injury. Results: Significant changes were observed in the coagulation parameters, in the total numbers of white blood cells and platelets in groups I and II, compared to controls. Conclusion: The modified haemostatic dressings used in this study produced a strong procoagulant effect in pigs. This, together with high fibrinogen concentrations, which can cause DIC, require further studying.

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“…Most combat wounds are deep and extensive, and dressings have to be applied deeply in between tissues, mostly thick layers of muscle (6,7). This study evaluated three haemostatic dressing prototypes composed of various materials and substances that stimulate clotting at the wound site (8). The application of various materials and substances to promote clotting affects not only blood vessels, but also the surrounding muscle tissue.…”

mentioning

confidence: 99%

The Influence of Haemostatic Dressing Prototypes for the Emergency Services on the Histopathological Parameters of Porcine Muscle

Holak¹,

Adamiak²,

Babińska³

et al. 2019

In Vivo

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Background/Aim: Haemostatic dressings for the uniformed and rescue services are an integral part of lifesaving equipment for controlling post-traumatic haemorrhage. The aim of this study was to assess the influence of active constituent substances and materials of haemostatic dressings on muscle tissue and muscle regeneration after traumatic injury. Materials and Methods: Three hemostatic dressing prototypes were analysed: OBR/G/S sponge: dressing material sponge made of Na-Ca chitosan/algal composite microfibers and nanofibers; OBR/MBT/S: tactic gauze modified with a polymer mixture of Na-Ca chitosan/algal composite microfibers and nanofibers, impregnated with a moderate amount of procoagulants (22.9 g/m 2); and OBR/MS/S: seton gauze modified with a polymer mixture of Na-Ca chitosan/algal composite microfibers and nanofibers, impregnated with a moderate amount of procoagulants (18.0 g/m 2), with chitosan (ChitoClearhqg 95) and sodium alginate (Protanal LF10/60 FT) as the coagulants. The experiment was conducted on 20 pigs which were euthanised 24 h, 7 or 14 days after wound dressing. Samples of porcine muscle tissue were subjected to qualitative histopathological analysis. Results: Histopathological analysis of muscle tissues from the experimental pigs revealed that the application of modified seton (OBR/MS/S) produced the most satisfactory results. The observed changes were similar on all dates that samples were collected and in all experimental groups, and minor differences in their extent were observed between groups. Regenerative processes were most advanced, and retrograde changes were least apparent in animals treated with OBR/MS/S. Conclusion: Modified seton (OBR/MS/S) induced the least tissue reaction and was most effective in promoting tissue regeneration after injury.

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The effect of hemostatic dressing prototypes for the uniformed services on selected blood coagulation parameters in pigs

Cited by 4 publications

References 7 publications

The Use of Large Animal Models in Trauma and Bleeding Studies

The Use of Large Animal Models in Trauma and Bleeding Studies

The Effect of Haemostatic Dressing Prototypes for the Emergency Services in the Porcine Haemostatic System

The Influence of Haemostatic Dressing Prototypes for the Emergency Services on the Histopathological Parameters of Porcine Muscle

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