“…1 × 10 4 cells were typically seeded onto each well of the 96 well-plates overnight. Then, the cells were dealt with distinct densities (5,10,20,40,80, and 160 mg mL −1 ) of DOX, CuFePBA@-PEGMA@AS1411, CuFePBA@PEGMA@AS1411/DOX, CoFeP-BA@PEGMA@AS1411 and CoFePBA@PEGMA@AS1411/DOX for 12 hours, respectively. Aerward, the cells were cleaned and further cultured with DMEM for another 12 h. Then, 20 mL MTT solution (5.0 mg mL −1 ) was then added to per well and cultured for 4 h. Finally, the medium was removed and dimethyl sulfoxide (150 mL) was added into each well.…”