The effect of Haemonchus contortus and Trichostrongylus colubriforms infection on the ruminal microbiome of lambs

Correa, Patricia; Mendes, Lucas William; Lemos, Leandro Nascimento; Sampaio, Ana Cláudia Koki; Issakowicz, Juliano; McManus, Concepta; Tsai, Siu Mui; Faciola, A.P.; Abdalla, Adibe Luiz; Louvandini, Hélder

doi:10.1016/j.exppara.2021.108175

Cited by 14 publications

(23 citation statements)

References 70 publications

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“…These different results underline the complex and dynamic relationships between gastrointestinal nematodes, the microbiota and the host mucosal immune response. Besides, it has been shown in sheep and goats that H. contortus, Trichostrongylus colubriformis and Teladorsagia circumcincta infection increased the abundance of the genera Prevotella respectively, in the ruminal, fecal or abomasal microbiota 2 , 6 , 14 , 15 . The increased abundance of Prevotella in the abomasum, associated with nematode infection remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Metagenome reveals caprine abomasal microbiota diversity at early and late stages of Haemonchus contortus infection

Aboshady

Choury

Montout

et al. 2023

Sci Rep

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Haemonchus contortus is one of the most detrimental gastrointestinal nematode parasites for small ruminants, especially in tropics and subtropics. Gastrointestinal nematode and microbiota share the same microhabitat; thus they interact with each other and their host. Metagenomics tools provide a promising way to examine the alterations in the gastric microbial composition induces by gastrointestinal parasites. In this study, we used metagenomics tools to characterize the impact of H. contortus infection on the caprine abomasal microbiota at early and late stage of infection and compared it with non-infected control. Our results showed that H. contortus infection caused a significant increase in abomasal pH at early (7 days post-infection) and late stage of infection (56 days post-infection). The analysis of alpha and beta diversity showed that the microbiota diversity both in number and in proportion was significantly affected at early and late stage of infection. All microbiota classes are impacted by H. contortus infection but Clostridia and Bacteroidia are more concerned. In infected animals, the genera Prevotella decreased at 7 and 56 days post-infection. Here we showed that the abomasal microbiota was significantly affected early after H. contortus infection, and these changes persist at late stage of the infection.

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Section: Discussionmentioning

confidence: 99%

Metagenome reveals caprine abomasal microbiota diversity at early and late stages of Haemonchus contortus infection

Aboshady

Choury

Montout

et al. 2023

Sci Rep

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show abstract

“…At genus level, Prevotella has been described as the most affected by GINs infections caused by H. contortus, Trichostrongylus colubriformis and T. circumcincta in sheep and goats. An increase in relative abundance in obligate anaerobes taxa, as Prevotellaceae family in lambs, sheep and goats infected with H. contortus, T. colubriformis and T. circumcincta was detected in infected animals compared with non-infected animals [44][45][46][47] . It could be explain by the Prevotellarole in protein degradation and energy host metabolism compensation 44,48 .…”

Section: Discussionmentioning

confidence: 95%

“…www.nature.com/scientificreports/ Butyrate is a short-chain fatty acid (SCFA) that is formed during the microbial fermentation of dietary fiber of ruminants. This metabolite is present in low concentrations, and it seems to be involved not only in nutrition, but also as a potent inhibitor of intestinal inflammation 47 detected a decrease in metabolic pathway genes related to butyrate after an infection with H. contortus and T. circumcincta in lambs. Besides, this decrease in butyrate metabolism was following by the abundance reduction in some butyrate-producers bacteria species.…”

Section: Discussionmentioning

confidence: 99%

Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta

Agüero

Esteban-Blanco

Argüello

et al. 2022

Preprint

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Gastrointestinal nematodes (GIN) are a major threat to health and welfare in small ruminants worldwide. Teladorsagia circumcincta is a nematode that inhabits the abomasum of sheep, especially in temperate regions, causing important economic losses. Given that T. circumcincta and microbiome share the same niche, interactions between them and the host are expected. Although it is known that within a sheep breed there are animals that are more resistant than others to infection by GIN, it is not known if the microbiome influences the phenotype of these animals. Under this condition, 12 sheep were classified according to their cumulative faecal egg count (cFEC) at the end of a first experimental infection, 6 as resistant group (RG) and 6 as susceptible group (SG) to T. circumcincta infection. Then, all sheep were experimentally infected with 70,000 L3 of T. circumcincta and at day 7 days post-infection were euthanized. At necropsy, gastric mucosa and gastric content from abomasum were collected to extract bacterial DNA and sequence V3-V4 region from 16S rRNA gene using Ilumina technology. After bioanalysis performed, results showed that α-diversity and β-diversity remained similar in both groups. However, resistant phenotype sheep showed a higher number of bacteria butyrate-fermenting species as Clostridium sensu stricto-1 (abundance in RG: 1.29% and in SG: 0.069%; p = 0.05), and Turicibacter (abundance in RG: 0.31% and in SG: 0.027%;p = 0.07) in gastric content but also Serratia spp in gastric mucosa (abundance in RG: 0.12% and in SG: 0.041%; p = 0.07). A trend towards a significant negative correlation between cFEC and Clostridium sensu stricto-1 abundance in gastric content was detected (r = -0.537; p = 0.08). These data suggest that microbiome composition could be another factor that influence in the development of the resistant phenotype modifying the interaction with the host and the in last instance affecting the individual risk of infection.

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“…At genus level, Prevotella has been described as the most affected by GINs infections caused by H. contortus , Trichostrongylus colubriformis and T. circumcincta in sheep and goats. An increase in relative abundance in obligate anaerobes taxa, as Prevotellaceae family in lambs, sheep and goats infected with H. contortus, T. colubriformis and T. circumcincta was detected in infected animals compared with non-infected animals 44 – 47 . It could be explain by the Prevotella role in protein degradation and energy host metabolism compensation 44 , 48 .…”

Section: Discussionmentioning

confidence: 96%

“…Butyrate is a short-chain fatty acid (SCFA) that is formed during the microbial fermentation of dietary fiber of ruminants. This metabolite is present in low concentrations, and it seems to be involved not only in nutrition, but also as a potent inhibitor of intestinal inflammation 47 detected a decrease in metabolic pathway genes related to butyrate after an infection with H. contortus and T. circumcincta in lambs. Besides, this decrease in butyrate metabolism was following by the abundance reduction in some butyrate-producers bacteria species.…”

Section: Discussionmentioning

confidence: 99%

Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta

Agüero

Esteban-Blanco

Argüello

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Gastrointestinal nematodes (GIN) are a major threat to health and welfare in small ruminants worldwide. Teladorsagia circumcincta is a nematode that inhabits the abomasum of sheep, especially in temperate regions, causing important economic losses. Given that T. circumcincta and microbiome share the same niche, interactions between them and the host are expected. Although it is known that within a sheep breed there are animals that are more resistant than others to infection by GIN, it is not known if the microbiome influences the phenotype of these animals. Under this condition, 12 sheep were classified according to their cumulative faecal egg count (cFEC) at the end of a first experimental infection, 6 as resistant group (RG) and 6 as susceptible group (SG) to T. circumcincta infection. Then, all sheep were experimentally infected with 70,000 L3 of T. circumcincta and at day 7 days post-infection were euthanized. At necropsy, gastric mucosa and gastric content from abomasum were collected to extract bacterial DNA and sequence V3-V4 region from 16S rRNA gene using Ilumina technology. After bioanalysis performed, results showed that α-diversity and β-diversity remained similar in both groups. However, resistant phenotype sheep showed a higher number of bacteria butyrate-fermenting species as Clostridium sensu stricto 1 (abundance in RG: 1.29% and in SG: 0.069%; p = 0.05), and Turicibacter (abundance in RG: 0.31% and in SG: 0.027%; p = 0.07) in gastric content but also Serratia spp in gastric mucosa (abundance in RG: 0.12% and in SG: 0.041%; p = 0.07). A trend towards a significant negative correlation between cFEC and Clostridium sensu stricto 1 abundance in gastric content was detected (r = − 0.537; p = 0.08). These data suggest that microbiome composition could be another factor associated with the development of the resistant phenotype modifying the interaction with the host and the in last instance affecting the individual risk of infection.

show abstract

The effect of Haemonchus contortus and Trichostrongylus colubriforms infection on the ruminal microbiome of lambs

Cited by 14 publications

References 70 publications

Metagenome reveals caprine abomasal microbiota diversity at early and late stages of Haemonchus contortus infection

Metagenome reveals caprine abomasal microbiota diversity at early and late stages of Haemonchus contortus infection

Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta

Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta

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