The Effect of Gyejibokryunghwan on Fracture Union in Tibia-fractured Rats

Abstract

Objectives The object of this study was to assess the effect of Gyejibokryunghwan (GBH) on anti-oxidant and anti-inflammatory activities in RAW 264.7 cells and on factors associated with fracture union in tibia-fractured rats. Methods The 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity was measured to assess anti-oxidant activity. The production of nitric oxide (NO), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the RAW 264.7 cells were measured to assess anti-inflammatory activity. The production of osteocalcin, calcitonin, carboxy-terminal telepeptides of type II collagen (CTXⅡ), transforming growth factor-β (TGF-β), bone morphogenetic protein-2 (BMP-2) in serum of tibia-fractured rats were measured to assess the effects of fracture union. X-rays were taken every two weeks from 0 to 4th week to assess fracture union effect. Results DPPH radical scavenging activity of GBH was increased according to concentration of GBH in RAW 264.7 cell. NO, prostaglandin E2 (PGE2), IL-6, IL-1β and TNF-α were significantly decreased, indicating anti-inflammatory effect. Osteocalcin, calcitonin, TGF-β were significantly increased in the experimental groups. CTXⅡ was significantly decreased in the experimental groups. BMP-2 was not significantly changed in the experimental groups. The X-ray showed that the experimental group has better healing effects on tibia-fractured rats than control group. Conclusions From above result, GBH has an effect on anti-oxidant, anti-inflammatory activities in RAW 264.7 cells. GBH showed significant results in factors related with fracture union and radiologic examination. In conclusion, GBH can help fracture union and it well be expected to be used actively in clinics.

Effects of Extracts from Cnidium officinale and Angelica sinensis on Bone Fusion in Mice with Femoral Fracture

“…현재까지 골절 치료를 위한 다방면의 한약 연구가 보 고되었다. 방제를 활용한 연구로는 加味芎歸湯 33) , 加味 身痛逐瘀湯 14,15) , 順氣活血湯 16) , 復元活血湯 17,18) , 當歸鬚 散 19,20) , 破血散疼湯 21) , 血府逐瘀湯 22) , 桃紅四物湯…”

“…한의학 서적 중《外臺秘要》에서는 "救急療救骨折, 接 令如故"로 골절에 대한 병명을 처음 언급하였고 7) , ≪醫 宗金鑑≫에서는 부위별 보호대를 활용하는 방법을 제시 하였으며 8) , ≪聖濟總錄≫에서는 골절치료에 한약을 초 기에는 活血祛瘀, 중기에는 接骨續筋, 후기에는 補氣養 血, 健壯筋骨 하는 시기별 방법을 설명하였다 9) . 요 구성 약재로 쓰였을 뿐만 아니라 13) , 골절 유합에 효과 적임을 보고한 복합약물연구 加味身痛逐瘀湯 14,15) , 順氣活 血湯 16) , 復元活血湯 17,18) , 當歸鬚散 19,20) , 破血散疼湯 21) , 血府 逐瘀湯 22) , 桃紅四物湯 23) 를 이용하여 5 mm 간격으로 단순 결찰 봉합하였다. 반 대쪽 다리도 같은 방법으로 골절을 유발하였다(Fig.…”