The effect of growth regulators on the micro-shoots' regeneration of strawberries (Fragaria × ananassa Duch.) in in vitro culture

Abstract

The influence of various growth regulators on strawberry varieties' micro-shoots regeneration of Russian and foreign selection Bereginya, Tsaritsa, Urozhaynaya CGL, Frida, Asia, Kimberly, Honeoye, Marmolada has been established. The experiment involved micro-plants regenerated in in vitro culture. At the proliferation stage, 6-BAP, thidiazuron, kinetin, mival, succinic acid were added to the nutrient medium in specified concentrations to select the optimal growth regulator. The maximum response to the induction of additional shoots was obtained in explants cultivated on the control medium of Murashige and Skoog supplemented with 6-BAP. The positive effect of kinetin, thidiazuron, mival, succinic acid on the process of root formation was noted, which can accelerate the production of rooted plants ex vitro.

Effect of Different Plant Growth Regulators on In-vitro Regeneration in Varieties of Strawberry

“…In-vitro propagation of strawberry was first reported by Boxus in 1974 following which different types of medium, plant growth regulators, explants and genotypes has been used [8]. The types of growth regulators and genotype has a significant effect on the morphogenetic potential of cultured tissues [9]. Micropropagation of strawberry provides increase in vigour and rapid production of disease free strawberry plants which are usually true-to-type [10].…”