The effect of growth media and physical treatments on the adhesion properties of canine probiotics

Abstract

Aims: The manufacturing processes have been reported to influence the properties of probiotics with potential impact on health properties. The aim was to investigate the effect of different growth media and inactivation methods on the properties of canine-originated probiotic bacteria alone and in combination mixture. Methods and Results: Three established dog probiotics, Lactobacillus fermentum VET9A, Lactobacillus plantarum VET14A and Lactobacillus rhamnosus VET16A, and their combination mixture were evaluated for their adhesion to dog mucus. The effect of different growth media, one reflecting laboratory and the other manufacturing conditions, and inactivation methods (95°C, 80°C and UV irradiation) on the mucus adhesion of the probiotic strains was characterized. Evaluation of dog probiotics was supported by cell visualization using transmission electron microscopy (TEM). Higher adhesion percentage was reported for probiotic strains growing in laboratory rather than in manufacturing conditions (P < 0Á05). Inactivation by heat (95°C, 80°C) decreased the adhesion properties when strains were cultivated in soy-based growth media compared with those grown in MRS broth (P < 0Á05). TEM observations uncovered differences in cell-surface components in nonviable forms of probiotic strains as compared with their viable forms. Conclusions: Manufacturing process conditions such as growth media and pretreatment methods may significantly affect the adhesive ability of the tested strains. Significance and Impact of the Study: Growth conditions, growth media, pretreatment methods and different probiotic combinations should be carefully considered for quality control of existing probiotics and for identification of new probiotics for dogs. These may also have an impact on health benefits for the host.

Pathogen exclusion properties of canine probiotics are influenced by the growth media and physical treatments simulating industrial processes

“…We have recently demonstrated that these same probiotic bacteria of canine origin present different adhesive properties when cultivated in different media and inactivated by different treatments (Grześkowiak et al . ).…”

“…; Vahjen and Manner ; Grześkowiak et al . ). Our results show that all the pathogens tested have the ability to bind to intestinal mucus, which thus benefits the pathogens in invading the host.…”

“…Previous reports demonstrate that the viability of probiotics may affect the adhesion and pathogen exclusion (Ananta and Knorr ; Grześkowiak et al . ). Nonviable forms of probiotics may be less able to bind to intestinal mucosa but they are also less likely to improve their safety (Haller et al .…”

“…; Grześkowiak et al . ). Thus, it seems reasonable from veterinary practice and commercial point of view to use a mixture of probiotics than a single strains in animal health care.…”

“…; Grześkowiak et al . ), alone and in mixture on the exclusion properties of pathogenic bacteria such as Ent. canis, Salm.…”