The effect of growth hormone and insulin on limb‐bone rudiments of the embryonic chick cultivated in vitro

Effect of Insulin on Bone in Tissue Culture

“…This also confirms our earlier observations (Prasad & Reynold 1969) on the effect of insulin on bone repair in vitro. Hay (1958) demonstrated the effect of insulin on embryonic chick femurs in vilro and suggested that insulin at first stimulates the growth which is then followed by a diminution in length. The increase in length and weight of the bones treated with insulin as observed in our experiments suggests that it has little effect on normal growing explants (chick embryonic tibiae) in comparison with the control medium.…”

Effect of Insulin on the Healing of Bone Wounds in Albino Rats

“…Of the 31 experimental animals, 2 developed infections, 1 developed a pathological fracture, 1 escaped while being weighed, 7 died of hypoglycemia, and 4 were used for histologic studies, leaving 16 animals for bone breaking-strength tests. They were tested as follows: both the left and right femora were carefully dissected out and, after being radiographed, were subjected to the breaking-strength test.…”