The effect of growth hormone on the development of in vitro matured unstimulated human oocytes

Sophonsritsuk, Areepan; Suwajanakorn, Somchai; Pruksananonda, Kamthorn; Sereepapong, Wisan; Ahnonkitpanit, Vichuda; Virutamasen, Pramuan

doi:10.1016/s0015-0282(03)01198-1

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“…Building an IVM culture system with a higher success rate by adding effective ingredients has its clinical importance. In addition to the above-mentioned case report (26), it has only been reported that 1,000 ng/ml GH had no effect on IVM of human GV-stage oocytes, which were obtained during gynecologic surgery (31). Few studies have explored the optimal concentration of GH for human oocyte IVM.…”

Section: Discussionmentioning

confidence: 95%

Growth Hormone Promotes in vitro Maturation of Human Oocytes

Liu

et al. 2019

Front. Endocrinol.

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Increasing the success rate of in vitro maturation (IVM) for human oocytes has a major clinical significance. Previous studies have shown that growth hormone (GH) added into IVM medium could promote IVM of oocytes from non-human beings. However, few studies on systematic IVM for human oocytes with GH have been reported. Human germinal vesicle (GV) oocytes collected for IVM were cultured with different concentrations of GH to optimize the concentration. Metaphase II (MII) stage oocytes obtained from IVM were fertilized by intracytoplasmic sperm injection (ICSI). Maturation rate, fertilization rate, and blastocyst rate were assessed after IVM with or without GH. Furthermore, gene expression profiles were compared in oocytes between the two groups using single-cell RNA-seq. The optimal concentration of GH for IVM was 200 ng/ml, and the maturation rate of this group reached 70% which was double that of the control group (35%, P = 0.004). The fertilization rate (73.1 vs. 60.3%) and blastocyst rate (25.0 vs. 15.5%) both had an increasing trend in the GH group compared to controls. Single-cell RNA-Seq and real-time PCR data showed that GH could significantly enhance the expression of genes associated with meiotic progression and embryo development, such as AURKA (aurora kinase A, P = 0.007), PDIA6 (protein disulfide isomerase family A member 6, P = 0.007), LINGO2 (leucine rich repeat and Ig domain containing 2, P = 0.007), and CENPJ (centromere protein J, P = 0.039). Taken together, GH could promote maturation of human oocytes, probably through accelerating meiotic progression, balancing redox homeostasis of cellular environment, and promoting oocyte developmental competence.

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Section: Discussionmentioning

confidence: 95%

Growth Hormone Promotes in vitro Maturation of Human Oocytes

Liu

et al. 2019

Front. Endocrinol.

View full text Add to dashboard Cite

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The effect of growth hormone on the development of in vitro matured unstimulated human oocytes

Cited by 1 publication

References 0 publications

Growth Hormone Promotes in vitro Maturation of Human Oocytes

Growth Hormone Promotes in vitro Maturation of Human Oocytes

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