The effect of growth differentiation factor-5–coated sutures on tendon repair in a rat model

Dines, Joshua S.; Weber, L. James; Razzano, Pasquale; Prajapati, Rita; Timmer, Mark; Bowman, Stephen M.; Bonasser, Lawrence; Dines, David M.; Grande, Daniel P.

doi:10.1016/j.jse.2007.03.001

Cited by 121 publications

(119 citation statements)

References 24 publications

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“…Unoperated, contralateral tendons were used for biomechanical testing. Previous studies have shown that rhGDF-5 plays a well-documented role in the formation and healing process of tendons [5][6][7][8]17]. We used the biomechanical and histologic data generated from two of these previous studies to develop the grading scale which was the aim of this project [7,8].…”

Section: Methodsmentioning

confidence: 99%

Histologic Stages of Healing Correlate with Restoration of Tensile Strength in a Model of Experimental Tendon Repair

et al. 2010

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Much current research is focused on biologic enhancement of the tendon repair process. To evaluate the different methods, which include a variety of gene therapy and tissue engineering techniques, histological and biomechanical testing is often employed. Both modalities offer information on the progress and quality of repair; however, they have been historically considered as two separate entities. Histological evaluation is a less costly undertaking; however, there is no validated scoring scale to compare the results of different studies or even the results within a given study. Biomechanical testing can provide validated outcome measures; however, it is associated with increased cost and is more labor intensive. We hypothesized that a properly developed, objective histological scoring system would provide a validated outcome measure to compare histological results and correlate with biomechanics. In an Achilles tendon model, we have developed a histological scoring scale to assess tendon repair. The system grades collagen orientation, angiogenesis, and cartilage induction. In this study, histology scores were plotted against biomechanical testing results of healing tendons which indicated that a strong linear correlation exists between the histological properties of repaired tendons and their biomechanical characteristics. Concordantly, this study provides a pragmatic and financially feasible means of evaluating repair while accounting for both the histology and biomechanical properties observed in surgically repaired, healing tendon.

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Section: Methodsmentioning

confidence: 99%

Histologic Stages of Healing Correlate with Restoration of Tensile Strength in a Model of Experimental Tendon Repair

et al. 2010

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“…Several studies delivered growth factors locally to the tendon repair site via coated sutures or dissolved in a fibrin sealant. 7,8 Although such augmentations appear promising, growth factors are short-lived and cannot be active for long periods. Gene therapy offers another approach for accurate delivery of the growth factor of interest, 9 but safety concerns exist with regard to the use of viral vectors.…”

Section: Zhao Et Almentioning

confidence: 99%

Biological augmentation of rotator cuff repair using bFGF-loaded electrospun poly(lactide-co-glycolide) fibrous membranes

Zhao¹,

Zhao²,

Dong³

et al. 2014

IJN

107

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Clinically, rotator cuff tear (RCT) is among the most common shoulder pathologies. Despite significant advances in surgical techniques, the re-tear rate after rotator cuff (RC) repair remains high. Insufficient healing capacity is likely the main factor for reconstruction failure. This study reports on a basic fibroblast growth factor (bFGF)-loaded electrospun poly(lactide-co-glycolide) (PLGA) fibrous membrane for repairing RCT. Implantable biodegradable bFGF–PLGA fibrous membranes were successfully fabricated using emulsion electrospinning technology and then characterized and evaluated with in vitro and in vivo cell proliferation assays and repairs of rat chronic RCTs. Emulsion electrospinning fabricated ultrafine fibers with a core-sheath structure which secured the bioactivity of bFGF in a sustained manner for 3 weeks. Histological observations showed that electrospun fibrous membranes have excellent biocompatibility and biodegradability. At 2, 4, and 8 weeks after in vivo RCT repair surgery, electrospun fibrous membranes significantly increased the area of glycosaminoglycan staining at the tendon–bone interface compared with the control group, and bFGF–PLGA significantly improved collagen organization, as measured by birefringence under polarized light at the healing enthesis compared with the control and PLGA groups. Biomechanical testing showed that the electrospun fibrous membrane groups had a greater ultimate load-to-failure and stiffness than the control group at 4 and 8 weeks. The bFGF–PLGA membranes had the highest ultimate load-to-failure, stiffness, and stress of the healing enthesis, and their superiority compared to PLGA alone was significant. These results demonstrated that electrospun fibrous membranes aid in cell attachment and proliferation, as well as accelerating tendon–bone remodeling, and bFGF-loaded PLGA fibrous membranes have a more pronounced effect on tendon–bone healing. Therefore, augmentation using bFGF–PLGA electrospun fibrous membranes is a promising treatment for RCT.

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“…This report and others (37) provide significant evidence that GDFs have an effect on the regeneration or neoformation of tendon in the adult, as well as in tendon morphogenesis in the developing animal. Delivery of recombinant human GDF5 (rhGDF5) via sutures to the site of injured rat tendons resulted in enhanced healing and significantly higher ultimate tensile load and stiffness compared with control sutures containing no rhGDF5 (37).…”

Section: The Molecular Basis Of Tendon Neoformationmentioning

confidence: 99%

Molecular targets for tendon neoformation

Aslan¹,

Kimelman-Bleich²,

Pelled³

et al. 2008

J. Clin. Invest.

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Tendons and ligaments are unique forms of connective tissue that are considered an integral part of the musculoskeletal system. The ultimate function of tendon is to connect muscles to bones and to conduct the forces generated by muscle contraction into movements of the joints, whereas ligaments connect bone to bone and provide joint stabilization. Unfortunately, the almost acellular and collagen I-rich structure of tendons and ligaments makes them very poorly regenerating tissues. Injured tendons and ligaments are considered a major clinical challenge in orthopedic and sports medicine. This Review discusses the several factors that might serve as molecular targets that upon activation can enhance or lead to tendon neoformation.

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The effect of growth differentiation factor-5–coated sutures on tendon repair in a rat model

Cited by 121 publications

References 24 publications

Histologic Stages of Healing Correlate with Restoration of Tensile Strength in a Model of Experimental Tendon Repair

Histologic Stages of Healing Correlate with Restoration of Tensile Strength in a Model of Experimental Tendon Repair

Biological augmentation of rotator cuff repair using bFGF-loaded electrospun poly(lactide-co-glycolide) fibrous membranes

Molecular targets for tendon neoformation

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