The effect of grape seed extract and vitamin C feed supplementation on some blood parameters and HSP70 gene expression of broiler chickens suffering from chronic heat stress

Abstract

In this experiment, the effect of hydroalcoholic grape seed extract (GSE) and vitamin C feed supplementation on some blood parameters and heat shock protein 70 (<em>HSP70</em> gene) expression of broiler chickens suffering from chronic heat stress was investigated. Experimental diets included control diet (with no additive), 3 levels of GSE (150, 300, 450 mg/kg), and one level of vitamin C (300 mg/kg). Each diet was fed to 5 replicates of 12 male chicks each, from d 1 to 42. The birds suffered from chronic daily heat stress under 34±1°C temperature with 65 to 70% relative humidity for 5 h from 29 to 42 d of age. Results showed that 300 mg/kg GSE supplementation increased body weight of broilers both before and after heat stress condition (at 28 and 42 d, respectively). Also, birds fed 300 mg GSE/kg diet had higher European production efficiency factor during the whole period of the experiment. Supplementation of GSE decreased the concentration of serum glucose at 28 and 42 d; at 42 d (during heat stress condition) and at 450 mg/kg diet it decreased cholesterol, triglyceride, lowand very low density lipoprotein concentration of serum blood. Vitamin C supplementation decreased serum cholesterol concentration of broilers suffering from heat stress. <em>HSP70</em> gene expression in heart and liver of broilers reduced by GSE and vitamin C supplementation pre- and during chronic heat stress condition.

Effects of exogenous tannase enzyme on growth performance, antioxidant status, immune response, gut morphology and intestinal microflora of chicks fed grape pomace

“…In agreement with the current results, Ozgan (2008) stated that the plasma glucose level was not affected by the inclusion of 1.0% grape seed oil in laying hen diets. Similar to the results of this study, administration of GSE reduced blood levels of total TG, LDL-C and increased HDL-C in broilers (Hajati et al, 2015). The concentration of uric acid in broiler serum was not affected by dietary treatments (P >0.05).…”

“…Hughes et al (2005) and Lau & King (2003) reported growth depression in chickens fed diets containing grape seed extract (GSE). In contrast, Hajati et al (2015) reported that GSE supplementation at the levels of 300 mg/kg diet improved the bodyweight of broilers. Recent works by Brenes et al (2008) and Chamorro et al (2013) showed that concentrations of up to 6% GP and 0.25% GSE could be used in chicken feed without modification in performance, in the size of the digestive organs or the ileal digestibility of protein and in amino acids.…”

“…This agrees with studies that reported that including plant extracts rich in polyphenols such as rosemary, grape, citrus and marigold at 5 g/kg diet in rats (Gladine et al, 2007) and inclusion of GP in laying hen diets up to 6 percent by Kara et al (2015) has no effect on serum protein and cholesterol. Hajati et al (2015) reported that GSE supplementation (150, 300, 450 mg/kg) decreased the concentration of serum glucose. In contrast, in the current study, GP supplementation of chicken diets did not have asignificant impact on serum glucose.…”

Effects of grape pomace and vitamin E on performance, antioxidant status, immune response, gut morphology and histopathological responses in broiler chickens

“…Growth depression in chickens fed diets containing GSE (90.2% total phenolics) has been reported by Lau and King (2003) and Hughes et al (2005), whereas Hajati et al (2015) stated that GSE addition at the levels of 300 mg/kg diet improved body weight of broilers. Grape by-products have potential for use as functional ingredients in animal nutrition (Brenes et al, 2008).…”

<b>Appraisal of purple coneflower (<i>Echinacea purpurea</i>) extract on production performance, internal organs, and gut microflora of japanese quail

“…The presence of active ingredients and phenolic compounds can reduce numbers of intestinal pathogens, thus minimizing wasting the nutrients (Nasir & Grashorn, 2010b). The antibacterial, anti-oxidant, anti-inflammatory activity of herbal extract polyphenols have been reported previously ( Hajati, Hassanabadi, Golian, Nassiri-Moghaddam, & Nassiri, 2015a;2015b;Gessner et al, 2013;Liu et al, 2016). Ebrahimi, Rahimi and Khaki (2015) reported that EP alcoholic extract in drinking water (1:1000 v v -1 ) statistically increased the relative weight of the spleen and Bursa of Fabricius as lymphoid tissues.…”