The Effect of Gossypol on the Structure of Nucleosomes

Malyuchenko, N. V.; Кошкина, Д. О.; Коровина, А. Н.; Герасимова, Н. Г.; Kirpichnikov, Mikhaǐl P.; Studitsky, Vasily M.; Feofanov, Аlexey V.

doi:10.3103/s0096392520030050

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…The DNA templates were obtained through a polymerase chain reaction (PCR) using a plasmid containing the 603–42A nucleosome positioning sequence [ 43 ] and fluorescently labeled primers. The DNA templates 187 bp in length were obtained using primers published previously [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

“…EGCG (Sigma-Aldrich, Merck, Rahway, NJ, USA) was dissolved in 100% DMSO at the concentration of 5 mM and stored at −20 °C. The preparation of samples for the spFRET analysis was performed according to the protocols published previously [ 35 , 44 ]. Briefly, EGCG (0.4–50 µM) was mixed with nucleosomes (1 nM) in a buffer (150 mM KCl, 5 mM MgCl 2 , 1 mM β-ME, 20 mM Tris-HCl, pH = 7.5) and incubated for 20 min at room temperature (RT).…”

Section: Methodsmentioning

confidence: 99%

“…The preparation of samples for electrophoresis was performed using the protocols published earlier [ 35 , 44 ]. Briefly, for the electrophoresis aimed to study the integrity of the EGCG–nucleosome complexes, EGCG (6–100 µM) was added to nucleosomes (60 nM) in 0.01 M buffer for dialysis (10 mM NaCl, 10 mM Tris-HCl (pH 7.9), 0.1% NP40, 0.2 mM EDTA, 5 mM β-ME), incubated for 20 min, mixed with sucrose (final concentration 10%) and loaded into the 4% polyacrylamide gel.…”

Section: Methodsmentioning

confidence: 99%

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Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1

Andreeva,

Maluchenko,

Efremenko

et al. 2023

IJMS

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The natural flavonoid epigallocatechin gallate has a wide range of biological activities, including being capable of binding to nucleic acids; however, the mechanisms of the interactions of epigallocatechin gallate with DNA organized in chromatin have not been systematically studied. In this work, the interactions of epigallocatechin gallate with chromatin in cells and with nucleosomes and chromatosomes in vitro were studied using fluorescent microscopy and single-particle Förster resonance energy transfer approaches, respectively. Epigallocatechin gallate effectively penetrates into the nuclei of living cells and binds to DNA there. The interaction of epigallocatechin gallate with nucleosomes in vitro induces a large-scale, reversible uncoiling of nucleosomal DNA that occurs without the dissociation of DNA or core histones at sub- and low-micromolar concentrations of epigallocatechin gallate. Epigallocatechin gallate does not reduce the catalytic activity of poly(ADP-ribose) polymerase 1, but causes the modulation of the structure of the enzyme–nucleosome complex. Epigallocatechin gallate significantly changes the structure of chromatosomes, but does not cause the dissociation of the linker histone. The reorganization of nucleosomes and chromatosomes through the use of epigallocatechin gallate could facilitate access to protein factors involved in DNA repair, replication and transcription to DNA and, thus, might contribute to the modulation of gene expression through the use of epigallocatechin gallate, which was reported earlier.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1

Andreeva,

Maluchenko,

Efremenko

et al. 2023

IJMS

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On the Interaction of Resveratrol with Nucleosomes

Maluchenko,

Andreeva,

Geraskina

et al. 2023

BIOPHYSICS

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Male infertility: before and after the era of SARS-CoV-2

et al. 2020

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Резюме Установлено, что мужчины болеют коронавирусом в 1,5 раза чаще, чем женщины, но в то же время 95% из них переносят инфекцию в легкой форме. Существует вопросы, которые требуют научного объяснения: почему мужчины болеют чаще и более восприимчивы, чем женщины, проходит ли вирус через гемато-тестикулярный барьер, проникает ли он в семенную жидкость, если ли влияние SARS-CoV-2 на выработку андрогенов, поражается ли яичко и каковы последствия этого поражения, каково непосредственное влияние вируса и связанных с ним проблем (таких как социальные проблемы, изоляция, карантин, психологические проблемы, малоподвижный образ жизни и пр.), прежде всего на сексуальную функцию, сексуальное поведение и репродукцию в целом? В обзорной статье рассмотрена ситуация, связанная с пандемией коронавируса SARS-CoV-2 в 2020 г. и ее влиянием на мужское здоровье. Приводятся данные по эпидемиологии, аспектам патогенеза, особенностям новой коронавирусной инфекции у мужчин. Озвучены рекомендации международных обществ по репродукции (ASRM, ESHRE, РАРЧ) по проведению программ вспомогательных репродуктивных технологий (ВРТ) в эпоху пандемии. Представлены разные точки зрения на влияние тестостерона на заболеваемость мужчин. Показана важность оксидативного стресса сперматозоидов и антиоксидантной терапии мужского бесплодия. Изложены задачи международного проекта по изучению влияния коронавирусной инфекции SARS-CoV-2 и ее последствий на здоровье мужчин. Опубликовано приглашение к сотрудничеству с отечественными и зарубежными специалистами в области урологии и андрологии на базе ФГБУ «Национальный медицинский исследовательский центр акушерства, гинекологии и перинатологии имени академика В.И. Кулакова» Министерства здравоохранения Российской Федерации.

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The Effect of Gossypol on the Structure of Nucleosomes

Cited by 4 publications

References 20 publications

Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1

Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1

On the Interaction of Resveratrol with Nucleosomes

Male infertility: before and after the era of SARS-CoV-2

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