The effect of gold nanoparticle size on osteogenic differentiation of adipose-derived stem cells

Ko, Wan‐Kyu; Heo, Dong Nyoung; Moon, Hojin; Lee, Sang Jin; Bae, Min Soo; Lee, Jung Bok; Sun, In Cheol; Jeon, Hoon Bong; Park, Hun Kuk

doi:10.1016/j.jcis.2014.08.058

Cited by 158 publications

(128 citation statements)

References 37 publications

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“…Regarding ALP levels, nanoparticles may therefore be better carriers owing to their positively charged surfaces that allow for better cell adhesion and internalization compared with bioceramic materials, and their enhanced surface area for binding of cells as a result of their spherical nano-structure. These results are in line with Zhang et al [19] and Ko et al [20], who have found that the ALP activity of cultured primary osteoblasts was increased in a time-and dose-dependent manner in the presence of gold nanoparticles (AuNPs) and suggested that the size of nanoparticles maybe the critical factor in regulating osteoblasts activities. Overall, our data clearly demonstrated that the prepared nanoparticles positively accelerated osteoblast differentiation with higher ALP activity.…”

Section: Alkaline Phosphatase Activitysupporting

confidence: 90%

Preparation and Evaluation of Vancomycin-Loaded N-trimethyl Chitosan Nanoparticles

Shou³

et al. 2015

Polymers

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Chronic intracellular infections caused by drug-resistant pathogens pose a challenge to the treatment of chronic osteomyelitis. Such treatment requires an intracellular delivery system for the sustained release of antibiotics such as vancomycin (VCM), which is an antibiotic of last resort used against many clinically resistant bacteria. In this work, we report VCM-loaded N-trimethyl chitosan (TMC) nanoparticles and their potential application for drug delivery. The results showed that the prepared nanoparticles were predominantly spherical in shape with an average particle diameter of 220 nm, a positive zeta potential, and a loading efficiency of 73.65%˘1.83%. Furthermore, their drug release profile followed the Higuchi model for sustained release, with non-Fickian diffusion. Over a 24-h period, 6.51%˘0.58% of the drug within the optimized nanoparticles was released. In vitro cytology showed that osteoblasts (OBs) exhibited higher alkaline phosphatase activity (ALP) after exposure to TMC nanoparticle material. Furthermore, TMC nanoparticles increased the uptake of water-soluble quantum dots (QDs) by OBs, and both nanoparticles and VCM/TMC mixtures improved OB proliferative activity. We also investigated the minimum inhibitory concentration (MIC, 60 µg/mL), half maximal inhibitory concentration (IC50, 48.47 µg/mL), diameter of inhibition zone (DIZ, 1.050 cm), Polymers 2015, 7 1851 and turbidimetric (TB) assay of nanoparticles. All data demonstrated that VCM/TMC nanoparticles had excellent antibacterial activity against the Gram-positive bacterium Staphylococcus aureus. These findings suggest that VCM-loaded TMC nanoparticles have good potential for the sustained delivery of antibiotics to bone infections.

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Section: Alkaline Phosphatase Activitysupporting

confidence: 90%

Preparation and Evaluation of Vancomycin-Loaded N-trimethyl Chitosan Nanoparticles

Shou³

et al. 2015

Polymers

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“…Li et al 24 compared the pro-osteogenic effects of 40, 70 and 110 nm AuNPs and found that 40 and 70 nm AuNPs were more effective. Similar results were obtained in another study, where the osteogenic effects of 30 and 50 nm AuNPs were superior to those of smaller (15nm) and bigger (75 and 100 nm) AuNPs 25. In contrary, Zhang et al 13 observed that the osteogenic effect of 20 nm AuNPs on primary osteoblasts was better than that of 40 nm AuNPs.…”

Section: Introductionsupporting

confidence: 84%

Size-dependent Effects of Gold Nanoparticles on Osteogenic Differentiation of Human Periodontal Ligament Progenitor Cells

et al. 2017

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Gold nanoparticles (AuNPs) have been reported to promote osteogenic differentiation of mesenchymal stem cells and osteoblasts, but little is known about their effects on human periodontal ligament progenitor cells (PDLPs). In this study, we evaluated the effects of AuNPs with various diameters (5, 13 and 45 nm) on the osteogenic differentiation of PDLPs and explored the underlying mechanisms. 5 nm AuNPs reduced the alkaline phosphatase activity, mineralized nodule formation and expression of osteogenic genes, while 13 and 45 nm AuNPs increased these osteogenic markers. Compared with 13 nm, 45 nm AuNPs showed more effective in promoting osteogenic differentiation. Meanwhile, autophagy was up-regulated by 13 and 45 nm AuNPs but blocked by 5 nm AuNPs, which corresponded with their effects on osteogenic differentiation and indicated that autophagy might be involved in this process. Furthermore, the osteogenesis induced by 45 nm AuNPs could be reversed by autophagy inhibitors (3-methyladenine and chloroquine). These findings revealed that AuNPs affected the osteogenic differentiation of PDLPs in a size-dependent manner with autophagy as a potential explanation, which suggested AuNPs with defined size could be a promising material for periodontal bone regeneration.

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“…AuNPs have been widely used to influence cell behavior due to their ease of preparation and adaptability in surface functionalization. Specifically, AuNPs (15-100 nm in diameter) have been studied for their ability to promote osteogenic induction in MSC in a size-dependent manner [70]. Although all sizes gave rise to osteoblasts, those ranging between 30-50 nm were preferentially up taken as well as the most effective in inducing osteogenic differentiation of AD-MSC.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Nanotechnology for mesenchymal stem cell therapies

Corradetti

Ferrari

2016

Journal of Controlled Release

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The effect of gold nanoparticle size on osteogenic differentiation of adipose-derived stem cells

Cited by 158 publications

References 37 publications

Preparation and Evaluation of Vancomycin-Loaded N-trimethyl Chitosan Nanoparticles

Preparation and Evaluation of Vancomycin-Loaded N-trimethyl Chitosan Nanoparticles

Size-dependent Effects of Gold Nanoparticles on Osteogenic Differentiation of Human Periodontal Ligament Progenitor Cells

Nanotechnology for mesenchymal stem cell therapies

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