The effect of glufosinate on nitrogen assimilation at the physiological, biochemical and molecular levels in Phaeodactylum tricornutum

Xie, Jun; Bai, Xiaocui; Li, Yali; Sun, Chongchong; Qian, Haifeng; Fu, Zhengwei

doi:10.1007/s10646-014-1285-8

Cited by 21 publications

(14 citation statements)

References 33 publications

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“…5). On the other hand, faster growing phytoplankton cells often need more nitrogen to synthesize more cellular enzyme that can efficiently transform the obtained inorganic substances from environments to their own cellular organic matter (Xie et al 2014), additionally indicated by the lower P:N molar ratio in the exponential phase compared to that of stationary and decay phases (Fig. 3B).…”

Section: Discussionmentioning

confidence: 99%

The increasing aluminum content affects the growth, cellular chlorophyll a and oxidation stress of cyanobacteria Synechococcus sp. WH7803

Shi

Zhou

et al. 2015

Oceanological and Hydrobiological Studies

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I n t e r n a t i o n a l J o u r n a l o f O c e a n o g r a p h y a n d H y d r o b i o l o g y AbstractEffects of marine aluminum (Al) on phytoplankton are controversial, making it important to elucidate the mechanisms underlying Al effects. This study was aimed at identifying the effects of Al on the growth, chlorophyll a (chl a) content and the antioxidant mechanism of cyanobacteria Synechococcus sp. WH7803. The growth rate increased from 0.33 to 0.52 d -1 in media with the increasing Al concentration from 0.2 (control) to 20 μmol l -1 and almost saturated to 0.44 d -1 at ~ 0.5 μmol Al l -1 . The higher growth resulted in the higher biomass in both stationary and decay phases in the conditions of higher Al content. Chl a per cell reached 10.19 μg cell -1 in the exponential phase at 20 μmol Al l -1 , approximately 1.6 and 3.1 times higher than those in stationary and decay phases, respectively, and chl a per cell showed a similar pattern as a growth rate when plotted with Al content. Al addition increased the cellular methane dicarboxylic aldehyde (MDA) content in the exponential phase and decreased the superoxide dismutase (SOD) activity in the decay phase. In particular, our results indicated a positive relationship between chl a per cell and the growth rate, suggesting the stimulation of increasing Al on the growth of Synechococcus is related to the enhancement of cellular chl a content.

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Section: Discussionmentioning

confidence: 99%

The increasing aluminum content affects the growth, cellular chlorophyll a and oxidation stress of cyanobacteria Synechococcus sp. WH7803

Shi

Zhou

et al. 2015

Oceanological and Hydrobiological Studies

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“…qRT-PCR analysis was performed on CFX96 Touch TM Deep Well Real-Time PCR Detection System (Bio-rad, America). The gene coding β-actin was selected as an internal control (Xie et al, 2014). The sequences of target genes were obtained from KEGG database 1 and primers shown in Table 1 were designed by NCBI.…”

Section: Quantitative Real Time Pcrmentioning

confidence: 99%

Improving Fucoxanthin Production in Mixotrophic Culture of Marine Diatom Phaeodactylum tricornutum by LED Light Shift and Nitrogen Supplementation

Yang

Dong

2020

Front. Bioeng. Biotechnol.

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Fucoxanthin (Fx), a kind of primary carotenoids in brown seaweeds and diatoms, has attractive efficacy in human's healthcare including loss weight, the prevention of diabetes and Alzheimer's disease. Marine diatom Phaeodactylum tricornutum is now realized as a promising producer for commercial Fx production due to its higher content of Fx than brown seaweeds with easily artificial cultivation and Fx extraction. In the present study, to improve Fx production in P. tricornutum, the mixotrophic cultures were applied to optimize initial cell density, light intensity, light regime and nitrogen supplementation. The results showed that the higher initial cell density (1 × 10 7 cells mL −1) and lower light intensity (20 µmol m −2 s −1) were favorable for biomass production and Fx accumulation. The maximal Fx content [16.28 mg g −1 dry weight (DW)] could be achieved under blue light (BL), but the highest biomass concentration (5.53 g L −1) could be attained under red: blue light (R: B, 6:1) in the batch culture. A novel twophase culture approach was developed to increase the biomass concentration to the highest value (6.52 g L −1) with the maximal productivity of Fx (8.22 mg L −1 d −1) through light shift from R:B ratio (6:1) in phase 1 to R:B ratio (5:1) by enhancing BL and tryptone addition in phase 2. The content and intracellular amount of Fx were also increased 8% and 12% in phase 2 compared to phase 1. The expression levels analysis revealed that genes encoding phytoene synthase (PSY), zeaxanthin epoxidase (ZEP), and fucoxanthin-chlorophyll-protein b (FCPb) were upregulated significantly, with downregulation of the gene encoding violaxanthin de-epoxidase (VDE), leading to the improvement of Fx in phase 2. The present study demonstrated the two-phase culture strategy could promote Fx productivity through enhancing biomass production and increasing Fx content, indicating that strengthening BL coupled with adding tryptone were effective to facilitate Fx production by mixotrophic cultivation of marine diatom P. tricornutum.

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“…Although the effect of herbicides on NR has been studied in plants, there are not many data on this subject in microalgae, but decreases in NR due to exposure to different herbicides have been reported (Fayez & Abd-Elfattah, 2007;Prado et al, 2009). However, NR activity was not significantly affected by the herbicide glufosinate in the marine diatom Phaeodactylum tricornutm (Xie et al, 2014). Transcriptomic analyses carried out by RNA-Seq technique in C. reinhardtii cells exposed to atrazine have also shown a downregulation of NIT1 gene which encodes a nitrate reductase (Esperanza et al, 2015).…”

Section: Discussionmentioning

confidence: 97%

“…The expression, stability and activity of NR are subject to complex regulations. The activation level of NR is influenced by environmental factors, including the presence of different pollutants (Mosulen et al, 2003;Lillo et al, 2004;Fayez & Abd-Elfattah, 2007;Prado et al, 2009;Xie et al, 2014). Results obtained with C. reinhardtii show that the NR activity is extremely sensitive to atrazine ( Fig.…”

Section: Discussionmentioning

confidence: 98%

Atrazine induced changes in elemental and biochemical composition and nitrate reductase activity in Chlamydomonas reinhardtii

Fernández-Naveira

Rioboo

Cid

et al. 2016

European Journal of Phycology

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Herbicides play an important role in agricultural practices but the introduction of these compounds into the aquatic environment can have severe consequences for non-target organisms such as microalgae. The ubiquitous green freshwater microalga Chlamydomonas reinhardtii, a model species in all aspects of microalgal physiology, was used to assess the toxicity of atrazine, one of the most widely used herbicides throughout the world. Atrazine acts on photosynthesis and therefore can affect non-target primary producers, such as microalgae.Growth, dry weight, elemental composition, photosynthetic pigments and protein contents and nitrate reductase activity were studied. After 96 h of exposure to different atrazine concentrations all the parameters studied were affected, but different sensitivities to the herbicide were shown. Nitrate reductase (NR) activity was strongly affected even at an atrazine concentration that did not affect growth (0.1 µM); the lowest concentrations of atrazine assayed (0.1 and 0.25 µM) provoked a > 40% decrease in NR activity and NR decreased > 80% with atrazine concentrations of 0.5 µM. C/N ratio was also affected by all the atrazine concentrations assayed. Nitrate reductase activity and C/N ratio were better indicators of the cellular stress state than data on other biochemical components or growth rate. Among cell parameters assayed, the NR activity stood out as a sensitive cytotoxicity endpoint and the activity of this enzyme can be suggested as a sensitive biomarker of stress induced by atrazine in C. reinhardtii.

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The effect of glufosinate on nitrogen assimilation at the physiological, biochemical and molecular levels in Phaeodactylum tricornutum

Cited by 21 publications

References 33 publications

The increasing aluminum content affects the growth, cellular chlorophyll a and oxidation stress of cyanobacteria Synechococcus sp. WH7803

The increasing aluminum content affects the growth, cellular chlorophyll a and oxidation stress of cyanobacteria Synechococcus sp. WH7803

Improving Fucoxanthin Production in Mixotrophic Culture of Marine Diatom Phaeodactylum tricornutum by LED Light Shift and Nitrogen Supplementation

Atrazine induced changes in elemental and biochemical composition and nitrate reductase activity in Chlamydomonas reinhardtii

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