The Effect of Genistein on the Plasma Membrane Integrity of Frozen Ongole Grade Bull Semen Based on Skim Milk – Soy Lecithin Extender

Prihantoko, Kurniawan Dwi; Yuliastuti, Fitriana; Haniarti, H.; Kusumawati, Asmarani; Widayati, Diah Tri; Budiyanto, Agung

doi:10.1088/1755-1315/465/1/012054

Cited by 4 publications

(6 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They will attack and collect electrons from unsaturated fatty acids that make up the phospholipids of cell plasma membranes. An autocatalytic reaction (chain reaction) can ultimately damage all phospholipids of the spermatozoa plasma membrane when it is not prevented (Prihantoko et al 2020). During semen collecting, dilution, and storage, lipid peroxidation occurs in the plasma membrane, which produces free radicals (Medica et al 2021;Rizkallah et al 2022).…”

Section: Effect Of Diluent On Spermatozoa Qualitymentioning

confidence: 99%

Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

2022

Int J Vet Sci

View full text Add to dashboard Cite

The success of artificial insemination in dogs is significantly influenced by the quality of the semen used. Furthermore, during storage at 5oC, the semen is attacked by free radicals, which adversely affect its quality. Moringa leaves, and carrot extracts are natural ingredients with high antioxidant content, expected to overcome free radical attacks and oxidative stress experienced by sperm during storage. Therefore, this study aims were to examine the effect of adding Moringa leaf extract or carrot juice in coconut water-egg yolk diluent on the sperm quality of Kintamani dogs. The method used a completely random design. Three treatment groups exist for each diluent. The base diluent consists of young coconut water-egg yolk (D1), the base diluent plus moringa leaves extract (D2), and the base diluent plus carrot juice (D3). The life spermatozoa were observed in eight sections, from 0 (control) to 84 hours with 12 hourly observations. Each treatment group was examined for spermatozoa progressive motility, viability, abnormalities, and intact plasma membrane. The results showed that D2 maintained semen quality significantly better (P<0.05) than D1 and D3. However, D3 was better than D1 (P<0.05). It was concluded that the addition of moringa leaves extract in coconut water-egg yolk diluent maintained the best quality of spermatozoa for 60 hours, with motility, viability, abnormality, and intact plasma membrane of 56.67±19.98, 60.83±18.63, 11.83±2, 64, and 55.83±17.45%, respectively.

show abstract

Section: Effect Of Diluent On Spermatozoa Qualitymentioning

confidence: 99%

Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

2022

Int J Vet Sci

View full text Add to dashboard Cite

show abstract

“…Meanwhile, reducing ROS production can be done by reducing oxygen tension and minimizing radiation exposure, dead spermatozoa and damaged spermatozoa in the semen. Prihantoko et al (2020) stated that the addition of genistein at concentrations of 1 µM and 2 µM resulted in higher motility, viability, and membrane integrity than control and significantly affected motility, membrane integrity, and acrosome integrity of Ongole bull semen. Elsayed et al, (2019) stated that the concentrations of genistein used in their research were 1, 5, 10, and 100 µM.…”

Section: Discussionmentioning

confidence: 99%

Effect of Genistein Supplementation in Tris-Aminomethane Base Extender on Characteristics of Pre-Freezing and Post-Thawed Sperm Ongole Grade Bull

Nugraha¹,

Putri²,

Ardiantoro³

et al. 2023

AAVS

View full text Add to dashboard Cite

Sperm undergo physiological changes during the cooling and freezing process, and especially significant changes are seen after freezing than after cooling. This research analyzed the effect of genistein supplementation in the basic diluent of tris-aminomethane on the quality of spermatozoa. Bulls aged 3-4 years used in this research. This research used an experimental method to examine the effect of genistein levels in the diluent on the characteristics of spermatozoa during pre-freezing at 4-5 0 C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 10 µM, 30 µM and 50 µM genistein treatment in the basic diluent tris aminomethane-egg yolk. Progressive motility post-thawing at 1 hour and 2 hours was highest at genistein concentration 30 µM (P<0.05). The effect of the antioxidant genistein on tris aminomethane -egg yolk diluent gave a significant effect on sperm viability in the Before Freezing (BF) phase at 1.5 hours, BF and PT at two hours (P<0.05). The addition of different genistein in diluent tris aminomethane -egg yolk was no significant effect (P>0.05) on sperm abnormality in both the BF and PT phases at 1 hours, 1.5 hours and 2 hours. The addition of genistein with a concentration of 30 µM was able to maintain better semen quality.

show abstract

“…Lecithin selain berasal dari kuning telur, juga dapat berasal dari kedelai. Beberapa penelitian yang menggunakan pengencer berbasis tris (Augusto et al, 2019;Zhao et al, 2021;Yotov, 2015) dan berbasis susu skim (Prihantoko et al, 2020) menunjukkan bahwa penggunaan soybean lecithin (SL) sebagai bahan pencegah cold shock dapat menggantikan kuning telur.…”

Section: Pendahuluanunclassified

“…Penelitian lain tentang pengencer Skim -Soybean Lecithin menunjukkan hasil berbeda. Penelitian tersebut menunjukkan post thawing motility diatas 50% pada penggunaan pengencer Skim -Soybean Lecithin (Prihantoko et al, 2020).…”

Section: Hasil Dan Pembahasan Karakteristik Semen Segarunclassified

Kualitas Semen Cair dan Semen Beku Kambing Peranakan Etawa (PE) pada Berbagai Jenis Pengencer

Drevian Meita Hardyastuti,

Mas Yedi Sumaryadi,

Dadang Mulyadi Saleh

et al. 2023

snppvp

View full text Add to dashboard Cite

Penggunaan pengencer komersial pada pembuatan semen beku kambing PE umum digunakan di Indonesia. Namun penggunaan pengencer komersial ini memiliki kelemahan, yaitu masa simpan yang pendek dan keterbatasan akses perolehan barang yang tidak tersedia setiap saat, terutama untuk balai inseminasi buatan daerah (BIBD). Untuk itu perlu diketahui tentang pengaruh beberapa jenis pengencer berbasis skim dan tris yang telah dikembangkan terhadap kualitas semen cair dan semen beku kambing PE, sehingga dapat menjadi alternatif ketika pengencer komersial tidak tersedia. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan perlakuan 5 jenis pengencer yaitu : P1 : Pengencer Andromed (Andromed) , P2 : Pengencer Skim + Kuning Telur (SKT), P3 : Pengencer Skim + 1% Soybean lecithin (SSL), P4 : Pengencer Tris + Kuning telur (TKT), P5 : Pengencer Tris + 1% Soybean lecithin (TSL). Semen segar yang digunakan berasal dari 1 ekor kambing PE yang dipelihara dan dikoleksi semennya sesuai SOP BIB Ungaran. Hasil analisis statistik menunjukkan bahwa jenis pengencer berpengaruh nyata terhadap motilitas, viabilitas dan persentase membran plasma utuh semen cair dan semen beku kambing PE (P <0,05). Motilitas, viabilitas dan persentase membran plasma utuh tertinggi didapatkan pada perlakuan jenis pengencer Andromed (P1) dan yang terendah pada perlakuan pengencer Skim-Soybean Lecithin (P3). Namun pada kondisi pengencer komersial tidak tersedia, jenis pengencer berbasis skim dan tris dengan kuning telur dapat dipergunakan sebagai alternatif pengencer untuk pembuatan semen beku kambing PE.

show abstract

The Effect of Genistein on the Plasma Membrane Integrity of Frozen Ongole Grade Bull Semen Based on Skim Milk – Soy Lecithin Extender

Cited by 4 publications

References 12 publications

Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

Effect of Genistein Supplementation in Tris-Aminomethane Base Extender on Characteristics of Pre-Freezing and Post-Thawed Sperm Ongole Grade Bull

Kualitas Semen Cair dan Semen Beku Kambing Peranakan Etawa (PE) pada Berbagai Jenis Pengencer

Contact Info

Product

Resources

About