The Effect of Genistein on Anastomotic Healing in Intestinal Ischemia/Reperfusion Injury

Akıncı, Ozan; Tosun, Yasin; Kepil, Nuray

doi:10.1016/j.jss.2022.07.039

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…[ 13 - 17 ] Some of the research in the literature is designed as ischemia-reperfusion studies. [ 18 - 20 ] This is the first arousal of the presented study since nothing could be found about the correlation between tissue healing and the use of different suture materials. To evaluate alternative suture options, the structural and absorbable properties of each suture material were questioned on the physical, biochemical, and histological consequences on intestinal anastomosis healing.…”

Section: Discussionmentioning

confidence: 98%

The comparison of the suture materials on intestinal anastomotic healing: an experimental study

Uzunlu

2023

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: This experimental comparative study was to evaluate the local effects of three different suture materials on intestinal anastomosis healing. METHODS: Ethical approval was obtained from the University of Ethical Committee (E-60758568-020-176720). A prospective, experimental comparative analysis was conducted on 24 rats. They were divided into three equal groups; Group 1 underwent colonic anastomosis with Vicryl suture material, Group 2 underwent colonic anastomosis with polypropylene suture; and Group 3 underwent colonic anastomosis with polydioxanone (PDS) suture. The second operation underwent the 7th post-operative day. Adhesion score, anastomotic leakage, anastomotic bursting pressure, hydroxyproline levels, and histopathologic examination were evaluated. RESULTS: All animals survived, and no leakage, intestinal obstruction, or wound infection was observed during the experiment. The adhesion score was evaluated according to the Diamond classification and same in all groups. Median anastomotic bursting pressure was 125.75 mmHg (10–241) in the Vicryl group, 159.25 mmHg (113–190) in the polypropylene group, and 154.50 mmHg (20–212) in the PDS group. Hydroxyproline tissue concentrations were in the Vicryl group 1699.92±220.8 ng/mg (range: 1509.81–2186.47), in the polypropylene group 1126.24±607.12 ng/mg (range: 53.22–1815.63), and 1547.86±335.2 ng/mg (range: 973.66–1973.2) in PDS group. There was no difference among groups regarding the inflammatory response evaluated by histopathology. There was no statistical significance in all variables evaluated. CONCLUSION: This experimental study demonstrates that suture materials did not worsen tissue healing during intestinal anastomosis. Absorbable, slowly-absorbable, and non-absorbable suture materials could be used safely in every situation.

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Section: Discussionmentioning

confidence: 98%

The comparison of the suture materials on intestinal anastomotic healing: an experimental study

Uzunlu

2023

Ulus Travma Acil Cerrahi Derg

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“…All of these studies required the use of one animal per sampling incident, which highlights the importance of techniques that optimise the use of experimental animals. [20][21][22][23][24][25][26][27][28][29] In our selective clamping IIRI-BK model, we obtained five samples from the same injured intestine, considerably reducing the number of rats and allowing kinetic studies. In addition, SI morphological damage and villus recovery were comparable with several previous results published by our group and others.…”

Section: Discussionmentioning

confidence: 99%

“…Among experimental studies focused on gastrointestinal diseases found in the literature, IIRI-and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats appears as a current model used by many authors and research groups around the world. [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] For this reason, we selected these two relevant pathologies to evaluate the experimental BK procedure in an intestinal injury scenario. IIRI was used for simulating a model of intestinal intussusception, strangulated hernia or intestinal transplantation.…”

Section: Step 2 Bk Model Applicationmentioning

confidence: 99%

“…For example, in the SI, superior mesenteric artery clamping model is currently used to evaluate IIRI, requiring one animal for each sample and study time. [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] The same situation occurs in the field of experimental colitis, where old and recent scientific articles use one animal in each LI sample collection. [30][31][32][33][34][35][36][37] For the reasons mentioned above, it is a challenge to develop models that enable reducing the number of animals involved in these studies.…”

Section: Introductionmentioning

confidence: 99%

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When less is more: Experimental Bishop–Koop technique for reduction in the use of laboratory animals for intestinal pathophysiological studies

et al. 2023

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The use of animals to gain knowledge and understanding of diseases needs to be reduced and refined. In the field of intestinal research, because of the complexity of the gut immune system, living models testing is mandatory. Based on the 3Rs (replacement, reduction and refinement) principles, we aimed to developed and apply the derived-intestinal surgical procedure described by Bishop and Koop (BK) in rats to refine experimental gastrointestinal procedures and reduce the number of animals used for research employing two models of intestinal inflammation: intestinal ischemia-reperfusion injury and chemical-induced colitis. Our results show the feasibility of the application of the BK technique in rodents, with good success after surgical procedure in both small and large intestine (100% survival, clinical recovery and weight regain). A considerable reduction in the use of the number of rats in both intestinal inflammation models (80% in case of intestinal ischemia-reperfusion damage and 66.6% in chemical-induced colitis in our experimental design) was achieved. Compared with conventional experimental models described by various research groups, we report excellent reproducibility of intestinal damage and functionality, survival rate and clinical status of the animals when BK is applied.

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“…De los estudios experimentales focalizados en patología de intestino delgado la IIR es un modelo de interés en la actualidad. (91)(92)(93)(94)(95)(96)(97)(98)(99)(100) Materiales y métodos: Se emplearon ratas Wistar hembras (peso 221 ± 13 gr). A las mismas se les proporcionó agua y alimento a demanda previo al acto quirúrgico.…”

Section: Bk Para El Estudio De La Iir Intestinalunclassified

Efectos del precondicionamiento isquémico abdominal multiorgánico en modelos experimentales de isquemia-reperfusión

Zárate¹

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El origen de esta línea de investigación se relaciona con la problemática observada en la clínica del trasplante de órganos sólidos en humanos. Desde el momento en que se inicia el procedimiento quirúrgico de extracción del órgano en el donante, la isquemia se asocia a un conjunto de mecanismos plausibles de lesionar al órgano a implantar, y surge entonces el precondicionamiento isquémico (PCI) como una posible herramienta de atenuación a la injuria en cuestión. El PCI consiste en someter al tejido a uno o más periodos de isquemia breve seguido de períodos de reperfusión previamente a un periodo de isquemia prolongado, con el fin de generar una respuesta adaptativa que enlentezca la tasa de muerte celular, y de este modo proteja al órgano en cuestión. Una forma de realizar PCI es por medio del clampeo de la vasculatura nutricia del órgano implicado. El PCI fue ampliamente estudiado asociado al trasplante de los distintos órganos abdominales en forma aislada en roedores, obteniéndose resultados alentadores tanto en intestino delgado, como en hígado, y riñón. Es de notar que, en la práctica clínica, el momento de la extracción implica el accionar de un grupo quirúrgico de ablacionistas para cada órgano que luego se va a implantar. Sumando a esto el escaso tiempo con que se cuenta para la procuración de órganos, debido a que la celeridad en la procuración se objetiva en una mejor funcionalidad del injerto en el paciente, se requiere entonces de una coordinación óptima tanto en el seno de cada grupo quirúrgico como así también en la articulación entre cada uno de ellos. Esto conlleva que de incorporarse el precondicionamiento como procedimiento quirúrgico en la práctica clínica, sea necesario para su implementación, que cumpla ciertos criterios. Entre los que se incluyen: que el método sea sencillo, fácilmente reproducible, que genere un beneficio al conjunto de órganos o al menos no genere daño o perjuicio en ninguno de ellos, y que no prolongue innecesariamente el proceso de procuración. De este modo, se planteó en el presente trabajo la posibilidad de aplicación del precondicionamiento, pero en forma multiorgánica. Realizando el clampeo de la arteria aorta abdominal en su origen, a fin de precondicionar en un tiempo todos los órganos abdominales, simplificando así el método.

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The Effect of Genistein on Anastomotic Healing in Intestinal Ischemia/Reperfusion Injury

Cited by 5 publications

References 30 publications

The comparison of the suture materials on intestinal anastomotic healing: an experimental study

The comparison of the suture materials on intestinal anastomotic healing: an experimental study

When less is more: Experimental Bishop–Koop technique for reduction in the use of laboratory animals for intestinal pathophysiological studies

Efectos del precondicionamiento isquémico abdominal multiorgánico en modelos experimentales de isquemia-reperfusión

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