The Effect of Gas-Chromatography Injection Parameters on the Analysis of Thiols and Polysulfides

Jones, Lewis L.; Nalli, Stefano M.

doi:10.1016/b978-0-12-398549-1.00074-x

Cited by 1 publication

(2 citation statements)

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“…This peak was identified as bis(2-methyl-3-furyl)disulfide by searching against the NIST14 database (match score 685). Thiols can be easily oxidized to disulfides in various food matrices. , The heat-induced interconversion between 2M3FS and its disulfide during hot GC injection has been previously demonstrated, and ideally, cold-on-column injection would be preferred for thermally labile analytes . For our H/C MDGC–MS/O instrument, the injector and the main host GC oven where the connecting capillaries were housed were both kept at 240 °C.…”

Section: Resultsmentioning

confidence: 99%

“…39,40 The heatinduced interconversion between 2M3FS and its disulfide during hot GC injection has been previously demonstrated, and ideally, cold-on-column injection would be preferred for thermally labile analytes. 41 For our H/C MDGC−MS/O instrument, the injector and the main host GC oven where the connecting capillaries were housed were both kept at 240 °C. The hot temperature for the main host GC oven was set to prevent any condensation occurring within the connecting capillaries and to maintain proper flow balance.…”

Section: Nonselective Lle and Phplc Fractionationmentioning

confidence: 99%

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Qualitative Screening of Volatile Thiols in Wine by Selective Silver Ion Solid-Phase Extraction with Heart-Cutting Multidimensional Gas Chromatography Mass Spectrometry/Olfactometry

Chen

Darriet

2022

J. Agric. Food Chem.

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The chemical analysis of odorous volatile thiols is intrinsically challenging. Substantial progress has been made in quantitative analysis of targeted thiols at ultra-trace concentrations (ng/L), but lesser analytical attention has been given to the qualitative screening of unknown thiols. This work presents a solid-phase extraction (SPE) method using a silver ion (Ag + )-based sorbent to isolate volatile thiols from red wine. This proposed Ag + SPE method was effective (recovery: 87−101% for four non-furan thiols and 35−49% for two furan thiols), simple, safe, and greatly reduced artifacts, testifying to its suitability as the sample preparation protocol for a qualitative screening experiment. Separation and detection were conducted using heart-cutting multidimensional gas chromatography coupled to mass spectrometry/olfactometry (H/C MDGC−MS/O). Key parameters including H/C width, main host oven temperature, and cryogenic trapping temperature were investigated for optimal instrument performance. The developed Ag + SPE H/C MDGC−MS/O strategy was readily applicable for qualitative screening of odorous volatile thiols in wine, as demonstrated by two case studies.

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Section: Resultsmentioning

confidence: 99%