The effect of Galleria mellonella hemolymph polypeptides on Legionella gormanii.

Chmiel, Elżbieta; Palusińska-Szysz, Marta; Zdybicka-Barabas, Agnieszka; Cytryńska, Małgorzata; Mak, Paweł

doi:10.18388/abp.2014_1933

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…The caterpillar larva (also called wax worms) of the Lepidopteran greater wax moth ( Galleria mellonella ) has become a widely adopted insect model to study several human pathogens including Listeria spp, 26 Streptococcus , 27 Legionella , 28 Campylobacter , 29 Yersinia , 30,31 Pseudomonas , 32-34 Enterococcus , 35 Staphylococcus , 36 Candida , 37,38 Aspergillus 39-41 and other bacterial and fungal pathogens. 42-45 The life cycle of this organism comprises a larval stage that transforms within 6–8 weeks into pupae and finally into moth.…”

Section: Introductionmentioning

confidence: 99%

TheGalleria mellonellalarvae as anin vivomodel for evaluation ofShigellavirulence

et al. 2017

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Shigella spp. causing bacterial diarrhea and dysentery are human enteroinvasive bacterial pathogens that are orally transmitted through contaminated food and water and cause bacillary dysentery. Although natural Shigella infections are restricted to humans and primates, several smaller animal models are used to analyze individual steps in pathogenesis. No animal model fully duplicates the human response and sustaining the models requires expensive animals, costly maintenance of animal facilities, veterinary services and approved animal protocols. This study proposes the development of the caterpillar larvae of Galleria mellonella as a simple, inexpensive, informative, and rapid in-vivo model for evaluating virulence and the interaction of Shigella with cells of the insect innate immunity. Virulent Shigella injected through the forelegs causes larvae death. The mortality rates were dependent on the Shigella strain, the infectious dose, and the presence of the virulence plasmid. Wild-type S. flexneri 2a, persisted and replicated within the larvae, resulting in haemocyte cell death, whereas plasmid-cured mutants were rapidly cleared. Histology of the infected larvae in conjunction with fluorescence, immunofluorescence, and transmission electron microscopy indicate that S. flexneri reside within a vacuole of the insect haemocytes that ultrastructurally resembles vacuoles described in studies with mouse and human macrophage cell lines. Some of these bacteria-laden vacuoles had double-membranes characteristic of autophagosomes. These results suggest that G. mellonella larvae can be used as an easy-to-use animal model to understand Shigella pathogenesis that requires none of the time and labor-consuming procedures typical of other systems.

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Section: Introductionmentioning

confidence: 99%

TheGalleria mellonellalarvae as anin vivomodel for evaluation ofShigellavirulence

et al. 2017

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“…In addition, ApoLp-III is involved in lipid transport and immunity. Recently, the protein was recovered by methanol extraction, purified and evaluated against three Legionella species: L. dumoffii, L. pneumophila , and L. gormanii ( Palusinska-Szysz et al, 2012 ; Chmiel et al, 2014 ; Zdybicka-Barabas et al, 2014 ). Antimicrobial assays demonstrated a difference in susceptibility among Legionella species.…”

Section: Natural Biocides: An Alternative Way To Control Lmentioning

confidence: 99%

Legionella pneumophila: The Paradox of a Highly Sensitive Opportunistic Waterborne Pathogen Able to Persist in the Environment

et al. 2016

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Legionella pneumophila, the major causative agent of Legionnaires’ disease, is found in freshwater environments in close association with free-living amoebae and multispecies biofilms, leading to persistence, spread, biocide resistance, and elevated virulence of the bacterium. Indeed, legionellosis outbreaks are mainly due to the ability of this bacterium to colonize and persist in water facilities, despite harsh physical and chemical treatments. However, these treatments are not totally efficient and, after a lag period, L. pneumophila may be able to quickly re-colonize these systems. Several natural compounds (biosurfactants, antimicrobial peptides…) with anti-Legionella properties have recently been described in the literature, highlighting their specific activities against this pathogen. In this review, we first consider this hallmark of Legionella to resist killing, in regard to its biofilm or host-associated life style. Then, we focus more accurately on natural anti-Legionella molecules described so far, which could provide new eco-friendly and alternative ways to struggle against this important pathogen in plumbing.

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“…Our previous study showed that L. dumoffii cultured in the presence of choline was more sensitive to the antimicrobial peptide defensin and apolipophorin III (apoLp-III) isolated from Galleria mellonella hemolymph [ 11 ]. ApoLp-III, i.e., an insect homolog of human apolipoprotein E exerted a bactericidal effect on L. gormanii cells at an 8-fold lower concentration, compared to L. dumoffii , possibly reflecting differences in the cell surface properties [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Unusual Lipid Components of Legionella gormanii Membranes

et al. 2022

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Legionella spp. cause Legionnaires’ disease with pneumonia as the predominant clinical symptom. L. gormanii is the second most prevalent causative agent of community-acquired pneumonia after L. pneumophila. The study aimed to characterize the lipidome of L. gormanii membranes and the importance of these analyses in bacterial chemotaxonomy. Lipidomic analyses based on ultra-high performance liquid chromatography-mass spectrometry allowed the detection of individual molecular species of a wide range of L. gormanii membrane lipids contained in the outer (OM) and inner membranes (IM). The lipid profile comprised glycerolipids (triglycerides, diglycerides), phospholipids (phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, cardiolipin), and sphingolipids (ceramides, hexosylceramides). The most abundant lipid fraction in the IM and OM were phospholipids. The lipidomic analysis showed that two independent phosphatidylcholine (PC) synthesis pathways operating in L. gormanii: the PE-methylation (PmtA) pathway and the PC synthase (Pcs) pathway. Comparison of the molecular profile of PC species contained in the lipids of L. gormanii membranes cultured on the medium, with and without exogenous choline, showed quantitative differences in the PC pool. An unusual feature of the L. gormanii lipids was the presence of ceramides and hexosylceramides, which are typical components of eukaryotic cells and a very small group of bacteria. To the best of our knowledge, this is the first report of the occurrence of ceramides in Legionella bacteria.

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The effect of Galleria mellonella hemolymph polypeptides on Legionella gormanii.

Cited by 8 publications

References 31 publications

TheGalleria mellonellalarvae as anin vivomodel for evaluation ofShigellavirulence

TheGalleria mellonellalarvae as anin vivomodel for evaluation ofShigellavirulence

Legionella pneumophila: The Paradox of a Highly Sensitive Opportunistic Waterborne Pathogen Able to Persist in the Environment

Unusual Lipid Components of Legionella gormanii Membranes

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