The effect of gabapentin and pregabalin on intestinal incision wound healing in rabbits

Abstract

Aim:To evaluate the macroscopic and histologic effects of pregabalin (PG) gabapentin (GB) on longitudinal intestinal wound healing in New Zealand rabbits.Materials and Methods:The animals were divided into three groups randomly; the control group (n=6), PG group (n=6) and GB group (n=6). All animals were premedicated with xylazine HCI, 5 mg/kg i.m. and general anaesthesia was performed by ketamine HCI 50 mg/kg i.m injection. A 4 cm incision in the caecum through median laparotomy was achieved under aseptic surgery. Intestinal wound was closed with double-sutured. All animals were received parenteral antibiotic treatment for 5 days. PG and GB groups were treated by PG (30 mg/kg, oral, daily) and GB (30 mg/kg, oral, daily) for 10 days respectively. Control group did not receive any treatment. The animals were euthanized on day 10 and the caecum was examined by laparotomy. Adhesion formation was observed, and tissue samples were taken from suture lines for histologic examination. Cellular infiltration (polymorphonuclear white blood cells and mononuclear cells), accumulation of connective tissue, vascularization and extent of necrosis were evaluated and scored separately for each of mucosal, submucosal, muscular and serosal layers of caecum.Results:Adhesions were more severe in the GB group compared to other groups. No statistically significant differences were detected among the three groups about the wound healing.Conclusion:It was suggested that the use of gabapentinoids had no significant effect on wound healing in patients undergoing gastrointestinal surgery and further studies with treatment periods longer than 10 days are needed.

Changes in histopathology and cytokeratin AE1/AE3 expression in skin graft with different time on Indonesian local cats

“…After migrating to wound site, fibroblast will begin to synthesize extracellular matrix. Connective tissue is the main protein of extracellular matrix and has a big role in wound recovery [ 13 , 17 ].…”

Should orthopedic surgeons consider the effects of gabapentin administration on bone healing while treating a long bone fracture: experimental study in a rat model

“…Accelerating the union of a fractured bone has been the major purpose of such studies as well as identifying the agents that negatively affect its progression [5–7, 23]. Although the effects of gabapentin on wound healing in rats [24] and intestinal incision wound healing in rabbits [25] have been tested, no study has examined the direct relationship between bone metabolism and gabapentin monotherapy. Therefore, the main purpose of the present study was to assess the radiographic, histological, and mechanical effects of gabapentin on bone healing in a rat model of femur fracture.…”

Wound healing effect of Anzer origin propolis specimens on rats’ intestinal incision

“…Wound healing is a complex process in which the tissue repairs itself (23,36). The wound healing process is similar in various tissues; however, it has some distinctive features in the gastrointestinal tract, for example, in bowel wounds, stretching time develops much earlier than for the skin ( 23) and collagen is synthesized by smooth muscle cells in intestinal wounds (23,36).…”

“…Macrophages and fibroblasts then migrate to the wound area. Macrophages regulate inflammation by releasing cytokines (5,16,23).…”

“…In addition, systemic and local factors play a role in the healing of bowel wounds (13,23). Important factors in the extracellular matrix are collagen fibers, fibroblasts, and immune cells that regulate wound strength in the early postoperative healing process (8,13,23).…”