The Effect of Fungal Elicitors on Hyoscyamine Content in Hairy Root Cultures of<i>Hyoscyamus muticus</i>

Sevón, N.; Suomalainen, Mette; Hiltunen, R.; Oksman-Caldentey, Kirsi-Marja

doi:10.1055/s-2006-959927

Cited by 11 publications

(20 citation statements)

References 1 publication

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to cell suspension cultures, secondary metabolite accumulation in hairy roots is not readily manipulated by alterations in medium composition (Payne et al 1987). However, we have earlier demonstrated that by adding chitosan to the medium, the hyoscyamine content in H. muticus hairy roots could be increased 5-fold (Sev6n et al 1992).…”

Section: Introductionmentioning

confidence: 87%

“…Agrobacterium rhizogenes strain LBA 9402 (gift from Prof. U. Nyman, Copenhagen) grown on YMB medium (Sev6n et al 1992) and A. tumefaciens C58CI pRTGUS 104 (constructed by U. Commandeur, BBA, Braunschweig) grown on solid Luria medium (5 g 1-1 yeast extract, 10 g 1-1 tryptone, 10 g 1-1 NaCI, 10 g 1-1 Difco Bacto agar, pH 7.0) were used to obtain hairy roots on Hyoscyamus muticus strain Cairo. The origin of the plant material, transformation method, and cultivation conditions of the root clones have been described elsewhere (Oksman-Caldentey et al 1991;Sev6n et al 1992).…”

Section: Bacterial Strains and Cell Culture Materialsmentioning

confidence: 99%

“…The origin of the plant material, transformation method, and cultivation conditions of the root clones have been described elsewhere (Oksman-Caldentey et al 1991;Sev6n et al 1992). Clones LBA1S and C58A were chosen for further study.…”

Section: Bacterial Strains and Cell Culture Materialsmentioning

confidence: 99%

“…The transformed roots of Hyoscyamus muticus L. are fast-growing in hormone-free medium, genetically stable and produce secondary metabolites characteristic of the intact plant (Oksman-Caldentey et al 1989;Sev6n et al 1992). Thus they might offer a suitable in vitro system for studying primary metabolism e.g.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Oksman-Caldentey

Sev�n

Vanhala

et al. 1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The effect of carbon and nitrogen sources on two well-established hairy root clones, LBA1S and C58A, of Hyoscyamus muticus strain Cairo, were investigated. Both clones exhibited completely different patterns with regards to their growth rate, hyoscyamine accumulation, and fatty acid contents. Clone C58A grew faster and yielded more biomass (17.4 g 1-1, in 21 days), but produced less hyoscyamine. The maximum hyoscyamine content (120 mg 1-1) in clone LBA1S was reached in 28 days. Neither of the clones could use lactose or fructose as the sole carbon source, nor ammonium as the sole nitrogen source. The growth in the medium containing glucose was significantly reduced compared to that containing sucrose. Clone LBA1S was sensitive to the changes in sucrose concentration and an increase in ammonium in the culture medium, whereas C58A tolerated these changes better but was more sensitive to the increase in total nitrogen. Lipid synthesis was active in the exponential growth phase, and the total fatty acid content varied from 5 to 34 mg g-I of dry root material. The major fatty acids were linoleic, palmitic and linolenic. There were considerable differences in the total amount of lipids and in their relative ratios when different nutrients were applied.Abbreviations: DW --dry weight, FA --fatty acids, FFA--free fatty acids, FW --fresh weight

show abstract

Section: Introductionmentioning

confidence: 87%

Section: Bacterial Strains and Cell Culture Materialsmentioning

confidence: 99%

Section: Bacterial Strains and Cell Culture Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Oksman-Caldentey

Sev�n

Vanhala

et al. 1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Examples include chitosan increasing the concentration of the anticholinergic alkaloids scopolamine and hyoscyamine in Brugmansia candida, from which they are commercially extracted for antispasmodics in the treatment of motion sickness [212]. The same response to chitosan was discovered in Hyoscyamus muticus with it produced a 5-fold increase in hyoscyamine content compared to controls [213]. Chitosan supplementation induced a three-fold increase in the steroid drug precursor diosgenin in Trigonella foenum-graecum hairy root cultures [214].…”

Section: Use Of Chitin-based Treatments In Non-field Agricultural Sysmentioning

confidence: 99%

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

2013

View full text Add to dashboard Cite

Abstract:In recent decades, a greater knowledge of chitin chemistry, and the increased availability of chitin-containing waste materials from the seafood industry, have led to the testing and development of chitin-containing products for a wide variety of applications in the agriculture industry. A number of modes of action have been proposed for how chitin and its derivatives can improve crop yield. In addition to direct effects on plant nutrition and plant growth stimulation, chitin-derived products have also been shown to be toxic to plant pests and pathogens, induce plant defenses and stimulate the growth and activity of beneficial microbes. A repeating theme of the published studies is that chitin-based treatments augment and amplify the action of beneficial chitinolytic microbes. This article reviews the evidence for claims that chitin-based products can improve crop yields and the current understanding of the modes of action with a focus on plant-microbe interactions.

show abstract

Paclitaxel production is enhanced in suspension‐cultured hazel (Corylus avellana L.) cells by using a combination of sugar, precursor, and elicitor

Rahpeyma

Moieni

Javaran

2015

Engineering in Life Sciences

View full text Add to dashboard Cite

Hazel (Corylus avellana L.) has recently been drawing attention as an alternative source of taxol. In the present study, the effects of sugar type, and different concentrations of phenylalanine (Phe) and vanadyl sulfate (V) on the production of taxol in C. avellana were investigated. A factorial experiment was used to optimize the concentrations of the precursor and elicitor. The cells were treated with Phe and V on the fourth day of culture and were harvested every 2 days until the 10th day. By increasing the Phe and V supply, taxol production increased during the culture period and the maximum level of 4.2 μg/g (dry weight) was obtained at day 10 by combining 3 μM of Phe and 0.05 and 0.1 mM of V in media supplemented with fructose (3%). The time course study on taxol production suggested that the appropriate time for using Phe is day 4 of culture, and day 8 for V. Overall, taxol production in C. avellana cell suspension culture was improved by the use of the combined strategy.

show abstract

The Effect of Fungal Elicitors on Hyoscyamine Content in Hairy Root Cultures ofHyoscyamus muticus

Cited by 11 publications

References 1 publication

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

Paclitaxel production is enhanced in suspension‐cultured hazel (Corylus avellana L.) cells by using a combination of sugar, precursor, and elicitor

Contact Info

Product

Resources

About