The effect of follicular fluid trace elements concentrations on intracytoplasmic sperm injection outcomes

Abstract

232.561 AE 28.389, P ¼ 0.0002 and P < 0.0001, One-way ANOVA). Expression levels of the pluripotent genes (Oct4, Sox2 and Klf4) were not affected by Nickel in 4-Cell embryos (P > 0.05, One-way ANOVA).CONCLUSIONS: Nickel disrupted blastocyst hatching in a dose-dependent manner. Cleavage stage embryo development was not impacted by exposure to Nickel at concentrations below 100mM. Down-regulation of pluripotent genes and repression of trophectoderm differentiation may mediate the detrimental effects of Nickel on embryo development.Supported by: By March of Dime Foundation (MOD 82713) and the Stanley H. Kaplan Fund of NYU School of Medicine.