The Effect of Fluorocarbon FC 43 on the Metabolism of Steroids during Perfusion of the Isolated Rat Liver

Höller, Michael; Breuer, H.

doi:10.1515/cclm.1975.13.7.319

Cited by 5 publications

(5 citation statements)

References 10 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…: Cochran test: P<O.Ol); this difference is in agreement with the difference found by Holler and Breuer (12) for oestrogens. However the corresponding intrinsic clearance was estimated to be 0.96 ml.minI.g:', which is in agreement with the intrinsic clearance calculated for the liposoluble compounds in the emulsion in the present study (1.07 ml.mint.g"}; that value is also in agreement with the corrected intrinsic clearance estimated for «gitoxin »» by RIA in perfusions with BSA and perfluorocarbon (0.84 ml.minl.g").…”

Section: Pharmacokineticssupporting

confidence: 91%

See 1 more Smart Citation

A further study of the pharmacokinetics of gitoxin in rabbit isolated liver: clearance of 3H-Gitoxin

Pellegrin

Lesne

1985

European Journal of Drug Metabolism and Pharmacokinetics

View full text Add to dashboard Cite

Hepatic clearance of 3H-gitoxin was studied in the rabbit using an isolated perfused liver technique with an emulsion of a perfluorocarbon. The liposoluble material in the perfusion medium was extracted with dichloromethane, and gitoxin was assayed in the extract by high performance liquid chromatography. Pharmacokinetic parameters were estimated for the liposoluble (dichloromethane soluble) material in the water phase obtained by centrifugation of the emulsion, for the liposoluble material and unchanged gitoxin in the total emulsion. Distribution and elimination half-lives of the liposoluble fraction in the water phase, were estimated to be 0.47 and 4.80 hours respectively, Vd to be 148 ml.g-1 and intrinsic clearance to be 1.16 ml.min-1.g-1; these parameters were compared with those of a previous study with unlabelled gitoxin. Distribution and elimination half-lives of the liposoluble compounds in the emulsion were estimated to be 0.48 and 4.62 hours, Vd to be 47 ml.g-1 and intrinsic clearance to be 1.07 ml.min-1.g-1; these data were compared with those of the liposoluble compounds in the water phase. Distribution and elimination half-lives of unchanged gitoxin in the emulsion were estimated to be 0.22 and 0.70 hour, Vd to be 59 ml.g-1 and intrinsic clearance to be 11.4 ml.min-1.g-1; these data were compared with those of the liposoluble compounds in the emulsion. The subcellular distribution of gitoxin and its metabolites in the liver indicated that 79% of the radioactivity was found in the soluble fraction, no significant binding occurring in the mitochondrial and microsomal fractions.

show abstract

Section: Pharmacokineticssupporting

confidence: 91%

“…Holler and Breuer (12) found that hepatic clearance of oestrogens was reduced in rat liver perfused with an emulsion of perfluorocarbon, in comparison with the clearance in livers perfused with a water phase. This difference is very likely to be due to the influence of the perfluorocarbon on the free fraction of drug.…”

Section: Pharmacokineticsmentioning

confidence: 99%

A further study of the pharmacokinetics of gitoxin in rabbit isolated liver: clearance of 3H-Gitoxin

Pellegrin

Lesne

1985

European Journal of Drug Metabolism and Pharmacokinetics

View full text Add to dashboard Cite

show abstract

“…Pulse rate was 280/min. An 18% (vol/vol) fluorocarbon emulsion was taken as perfu sion medium (Holler and Breuer, 1975). The aqueous phase of the medium contained the following ions (mmoll L): Na+ 140; K+ 3.5; Ca 2 + 1.5; Mg 2 + 0.7; Cl-116;…”

Section: Technique For Perfusion Of the Brainmentioning

confidence: 99%

Eicosanoids in Rat Brain during Ischemia and Reperfusion—Correlation to DC Depolarization

Tegtmeier

Weber

Heister

et al. 1990

J Cereb Blood Flow Metab

Self Cite

View full text Add to dashboard Cite

Summary:The effects of complete ischemia on cerebral arachidonic acid (AA) metabolism were investigated in the isolated perfused rat brain. During 12.5 min of isch emia, AA, 5-hydroxy-6,8, 11, 14-eicosatetraenoic acid, and 15-hydroxy-5 ,8,11, 13-eicosatetraenoic acid increased 129-, 4-, and 10-fold, respectively, while subsequent reperfusion for 30 min resulted in normalized levels inde pendently of the duration of preceding ischemia. Prosta glandin (PG) F2u, PGE2, PGD2, 6-keto-PGF,u, and thromboxane (Tx) B2 remained at preischemic levels dur ing 12.5 min of complete ischemia. However, at the end of subsequent reperfusion for 30 min, the levels of the

show abstract

“…The volume of distribution thereby being increased, and the clearance decreased. Such a decrease has been described for oestrogens by Holler (18).…”

Section: Discussionmentioning

confidence: 57%

Hepatic clearance of gitoxin: pharmacokinetic study on rabbit isolated liver. Influence of protein binding and comparison with digoxin

Pellegrin

Lesne

1983

European Journal of Drug Metabolism and Pharmacokinetics

View full text Add to dashboard Cite

Hepatic clearance of gitoxin has been studied in the rabbit and compared with that of digoxin using an isolated perfused liver technique. During 1.5 hour perfusions with a modified Krebs-Henseleit solution, gitoxin perfusate levels decreased biexponentially; the distribution and elimination half-lives were estimated to be 0.14 and 1.25 hour, Vd area to be 95.5 ml.g-1 and intrinsic metabolic clearance to be 1.98 ml.min-1.g-1. During 1.5 hour perfusions with modified Krebs-Henseleit solution containing 2.7% bovine serum albumin, gitoxin perfusate levels decreased monoexponentially. This is probably due to protein binding which moderates hepatic uptake so that distribution is not yet complete after 1.5 hour and it is therefore impossible to discriminate the two phases. This was confirmed by 5 hour perfusion experiments with an emulsion of perfluorocarbon in the modified Krebs-Henseleit solution also containing 2.7% bovine serum albumin, during which gitoxin levels decreased biexponentially. Distribution and elimination half-lives have been estimated to be 0.31 and 5.54 hours, Vd area to be 139 ml.g-1 and intrinsic metabolic clearance to be 1.36 ml.min-1.g-1. Gitoxin has been compared in these experimental conditions with digoxin, one of the most often used cardiotonic's. Distribution and elimination half-lives of digoxin were estimated to be 0.34 and 4.52 hours, Vd area to be 46.5 ml.g-1 and intrinsic metabolic clearance to be 0.17 ml.min-1.g-1. Other pharmacokinetic parameters (alpha, beta, V1, V2...) also have been calculated for these three types of perfusion experiments.

show abstract

The Effect of Fluorocarbon FC 43 on the Metabolism of Steroids during Perfusion of the Isolated Rat Liver

Cited by 5 publications

References 10 publications

A further study of the pharmacokinetics of gitoxin in rabbit isolated liver: clearance of 3H-Gitoxin

A further study of the pharmacokinetics of gitoxin in rabbit isolated liver: clearance of 3H-Gitoxin

Eicosanoids in Rat Brain during Ischemia and Reperfusion—Correlation to DC Depolarization

Hepatic clearance of gitoxin: pharmacokinetic study on rabbit isolated liver. Influence of protein binding and comparison with digoxin

Contact Info

Product

Resources

About