The Effect of Fatal Carbon Monoxide Poisoning on the Equilibria Between Cell Membranes and the Electrolyte Solution

Abstract

The effect of fatal carbon monoxide poisoning on equilibria between cell membranes and surrounding ions was described using a theoretical four-equilibria model. The model was developed to obtain parameters characterizing the interactions between solution ions and erythrocyte or thrombocyte membrane surface. The parameters are the total surface concentrations of both acidic and basic groups CA, CB and their association constants with solution ions KAH, KBOH. These parameters were used to calculate the theoretical values of surface charge density. The model was validated by comparison of these values to experimental data, which were determined from the electrophoretic mobility measurements of the blood cells. The experimental and theoretical surface charge density values agree at pH 2–8, and at higher pH, the deviation was observed.

The Equilibria in Lipid–Lipoic Acid Systems: Monolayers, Microelectrophoretic and Interfacial Tension Studies

“…The zeta potential depends on the molecular composition of the biological membrane and plays an essential role in understanding membrane-binding mechanisms and cellular uptake, such as drug delivery. This parameter is an essential property of a cell and is useful for comparing the surface properties between two cell types [35,36]. Changes in the zeta potential of a living cell can commonly occur, including alongside diseases in cells since the membrane zeta potential is influenced by many different factors, such as membrane composition.…”

“…Six measurements were made (each covering 100-200 series for a duration of 5 s) for each pH value for each sample. The experiments were carried out three times [16,35,36].…”

Equilibria Between Cell Membranes and Electrolyte Solution: Effect of Fatal Accidental Hypothermia

“…The other two equilibria are associated with negative species of phospholipids or proteins and sodium and hydrogen ions. The model is presented in full detail in previous papers (Dobrzyńska et al 2006 ; Kotyńska et al 2012 ; Petelska et al 2015 ).…”

“…This parameter strongly depends on environmental conditions such as pH and membrane composition. Changes of the membrane surface charge density values are observed in vitro [adding ionizable surfactants (McLaughlin and Harary 1976 ), altering the membrane composition] as well as in vivo [sudden unexpected death (Kotyńska et al 2012 ), fatal carbon monoxide poisoning (Szeremeta et al 2013 ; Petelska et al 2015 ), fatal accidental hypothermia (Szeremeta et al 2015 )] conditions.…”

Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia

“…As there is no sufficient number of professional literatures about the influence of hypothermia on the electrical properties of biological membranes, we researched the changes of surface charge density of blood cells after fatal accidental hypothermia. It is a continuation of a systematic study of the electrical properties of postmortem human erythrocyte and thrombocyte membranes, which was examined by Figaszewski and co-workers (Kotyńska et al 2012 ; Szeremeta et al 2013 ; Petelska et al 2015 ). The researchers conducted their experience using a microelectrophoresis method since it is one of the fundamental analytical tools in case of biological studies.…”