The Effect of Fat Rich Diet and Alcohol on Ion Concentration in Bile Fluid in Rats

Abstract

The metabolic alteration of fatty liver causes significant changes in the composition of bile fluid secreted by epithelial liver cells. These processes include both organic molecular and inorganic ion content changes in bile juice as well. In this work ion concentration differences of bile fluid in experimental hyperlipidemic rats compared to normal ones were monitored. Fatty liver in young male Wistar albino rats was induced by 2% cholesterol, 20% sunflower oil and 0.5% cholic acid added to the normal food and 3% alcoholic water was administered to rats for 9 days. The development of hyperlipidemy was detected by measuring serum parameters and fatty liver was proved by morphological investigations. The ion concentrations of bile fluid samples were determined by ICP-OES (inductively coupled plasma optical emission spectrometry). The bile fluid samples were digested with a mixture of HNO ! and H O . The results show that the concentration of calcium and phosphorus in bile juice increased significantly in hyperlipidemic rats compared to the control. Significant concentration changes have also been detected for chromium and manganese. The changes in metal ion metabolism also point to the damage of liver cells owing to metal complex evacuation.

Protective effect of CV247 against cisplatin nephrotoxicity in rats

“…After digestion of the samples in nitric acid (5 ml, 65%) and H 2 O 2 (2 ml, 30%), an inductively coupled plasma (IPC) optical emission spectrometric method was used for measuring the metal content in a Spectro Genesis ICP equipment (Spectro Analytical Instruments GmbH, Kleve, Germany). 41 For the standardization of equipment and measurements of elements, Spectro multielement and Spectrum 3D standards were used. A computer-guided TraceLab 50 type polarographic–voltammetric analyzer was used for the voltammetric determination of Se at −550 mV.…”

“…After digestion of the samples in nitric acid (5 ml 65%) and hydrogen peroxide (2 ml 30%), an inductively coupled plasma optical emission spectrometric (ICP-OES) method was used for measuring metal content 43 in a Spectro Genesis ICP equipment (Kleve, Germany). For the standardization of equipment and measurements of elements Spectro multielement and Spectrum 3D standards were used.…”

Magnesium sulfate against oxidative damage of membrane lipids: A theoretical model

“…As mentioned in the introduction, the way in which the MgSO 4 molecule exerts an antioxidative effect in many diseases related to LPO is unclear at present. Several experimental works have reported that magnesium compounds are effective hydroxyl radical scavengers . In this sense, calculations with different approaches for electronic structure methods were performed with small systems to validate the results by comparison between them and with other theoretical calculations from the literature.…”

“…However, due to their high mobility and reactivity, • OH radicals may be trapped by the MgSO 4 CIP, as shown below, to prevent the reaction with H atoms of unsaturated fatty acid residues. This is feasible because, as aforementioned, experimental researchers have found that the MgSO 4 salt is a hydroxyl radical scavenger …”

Alterations in the Content of Metal Elements and Fatty Acids in Hepatic Ischaemia–Reperfusion: Induction of Apoptotic and Necrotic Cell Death

“…After digestion of the liver samples (protein content: 10 mg/ml) with a mixture of nitric acid and hydrogen peroxide and dilution with deionised water, the concentration of seven elements (Ca, Cu, Fe, Mg, Mn, P, Zn) was determined. Three times 3 s integration time, blank subtraction and background correction were applied during the measurements [26].…”