The effect of extended blastocyst exposure of hyaluronan enriched transfer media on implantation rate in frozen embryo transfers

Abstract

Prospective cohort study. MATERIALS AND METHODS: We followed 93,775 women participating in the Nurses' Health Study II between 1991 and 2013 who had no prior history of cardiovascular disease, cancer, or diabetes and who reported the usual length and regularity of their menstrual cycles at ages of 14-17, 18-22, and 28-48 years. We obtained hazard ratios (HR) and 95% confidence intervals (CI) for the relationships between menstrual cycle characteristics and mortality from Cox proportional hazards models adjusted for relevant confounders including body mass index, race/ethnicity, and physical activity, and lifestyle factors.RESULTS: We documented 1679 deaths, including 828 from cancer, and 166 from cardiovascular disease, during 1,729,410 person-years of followup. After adjustment for various covariates, women reported that their menstrual cycles were always irregular between the ages of 14-17 and 18-22 were 21% [HR¼1.21 (95% CI: 1.04, 1.40)] and 34% [HR¼1.34 (95% CI: 1.08, 1.66)], respectively, more likely to die from any causes during follow-up than women reporting very regular menstrual cycles in the same age range. A similar relation was observed with irregular menstrual cycles between the ages of 28-48 years. Likewise, women reporting a current usual cycle length of 32-39 days or of R40 days were more likely to die from any causes during follow-up than women whose current usual cycle length was 26-31 days [HRs ¼1.23 (95% CI: 1.04, 1.45), and 1.28 (95% CI: 1.05, 1.55), respectively]. Elevated HR for cardiovascular and cancer mortality was also associated with longer menstrual cycle lengths (>32 days) between the ages of 28-48 years.CONCLUSIONS: Irregular and long menstrual cycles are associated with an increased risk of mortality.

Effect of Hyaluronic Acid-Enriched Media in Embryo Implantation

“…An inflated concentration of hyaluronan improves in-vitro growth and the number of cells in bovine embryos by increasing the viscosity of the culture medium. They also discovered that embryos cultivated with hyaluronan supplementation had greater survival rates following freezing [ 1 - 3 , 10 , 11 ]. Onestep with increased hyaluronic acid concentration can be used successfully as a macromolecule in a medium used for embryo transfer which gives outcome in high pregnancy and implantation rates in a recent study [ 12 ].…”

Embryo Glue: The Use of Hyaluronan in Embryo Transfer Media

“…22 Perez et al showed that increased exposure to EmbryoGlue had a positive impact on implantation rate and clinical pregnancy rate, with a statistically significant difference seen in subgroup analysis between exposure times of 1 hour and 2 or 3 hours to HETM (p < 0.05, n ¼ 119). 23 A recent Cochrane review reported the effects of adherence compound HA when used in functional concentrations in embryo transfer media (21 studies and 6,704 participants). 24 This review found that there is evidence to support improved clinical pregnancy and live birth rates with the use of HA in embryo transfer media compared with low concentration or no HA media (Relative Risk (RR): 1.21, 95% CI: 1.1-1.31, 10 randomized controlled trials, N ¼ 4,066, I 2 ¼ 33%, moderate-quality evidence), although it is important to note that the observed increase in multiple pregnancy rates is suggested to be due to embryo transfer policies rather than the HA supplementation.…”