The Effect of Exopolysaccharide-Producing Probiotic Strains on Gut Oxidative Damage in Experimental Colitis

Abstract

EPS-producing probiotic bacteria significantly attenuate oxidative stress in experimental colitis. Increased EPS production gives rise to a better probiotic function. These results suggest that EPS molecules could revaluate probiotic strains and exert their beneficial effects on the host and this may have a therapeutic potential.

Biosynthesis and Bioactivity of Exopolysaccharides Produced by Probiotic Bacteria

“…Few works have explored the bioactivity of the bacterial EPS in relation to some diseases, which can be directly related to the immune state of the individual; this is the case of the intestinal colitis (Sengül et al 2006;Rodriguez et al 2009), arthritis (Nowak et al 2012), and some carcinogenic processes (Oda et al 1983;Kitazawa et al 1991;Kim et al 2010). More recently, studies of EPS bioactivity are focused on determining its potential as antioxidants, or free radical scavenging agents, to diminish damages and loss of function in many tissues caused by different reactive oxygen species (Sengül et al 2011;Xu et al 2011). It is worth to note that these studies are preliminary, most have been done in vitro and there is not demonstration of the efficacy in humans; besides, no mechanism of action have been proposed in order to explain how bacterial EPS could carry out such activities upon the biological tissues.…”

Comparative Analysis of Lactulose and Fructooligosaccharide on Growth Kinetics, Fermentation, and Antioxidant Activity of Common Probiotics

“…Some reported benefits include enhanced immune system [5] and defense against pathogens [6], modulation of short-chain fatty acid (SCFA) production [7] and tight junction integrity [8], and mild improvement in iron status [9]. Proliferation of beneficial bacteria, usually lactobacilli and bifidobacteria, in the colon of the host may also be associated with reducing lipid peroxidation in colonic mucosa of intact mammals [10] [11]. The putative antioxidant effect might be due to the ability of the bacteria to scavenge free radicals, and/or an increase in antioxidant capacities of the colon contents.…”

Experimental immunology Immunosuppressive effect of systemic administration of Lactobacillus rhamnosus KL37C-derived exopolysaccharide on the OVA-specific humoral response

“…In our basal experimental system mice were injected intraperitoneally with the antigen (OVA), adjuvant (LPS) and cEPS. Therefore, in contrast to other experimental models, restricted to oral administration of both EPS and antigen [33,34] we could observe the effects of EPS on the systemic immune system. Importantly, we used the same route of OVA immunization, as the one we used previously for collagen to induce the CIA.…”