The effect of exogenous deoxyribonucleosides on thymidine incorporation in T4‐infected cells

The rate at which 3H thymidine is incorporated into DNA is increased in T4w-infected cells compared to wild-type when measured late in infection under conditions of low thymidine concentration. This increased DNA synthesis is sensitive to hydroxyurea but not to mitomycin C, and can be prevented by the addition of chloramphenicol early in infection. Also, DNA replicative intermediates isolated from T4w-infected cells late in infection sediment significantly faster than those isolated from wild-type-infected cells. In contrast, DNA replicative intermediates isolated from T4x- or T4y-infected cells sediment more slowly than those produced by wild-type T4. Cells coinfected with wild-type T4+ and T4x, y or w; or T4w and T4x or y, produce wild-type DNA replicative intermediates. Cells coinfected with T4x and T4y produce more slowly sedimenting DNA replicative intermedites. Cells coinfected with T4w and wild-type T4 show wild-type rates of DNA synthesis while cells coinfected with T4w and T4x or T4y show increased rates of DNA synthesis over that observed with wild-type alone.

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The effect of exogenous deoxyribonucleosides on thymidine incorporation in T4‐infected cells

Cited by 12 publications

References 15 publications

Single-stranded DNA binding properties of the uvsy recombination protein of bacteriophage T4

Single-stranded DNA binding properties of the uvsy recombination protein of bacteriophage T4

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Properties of the nonlethal recombinational repair deficient mutants of bacteriophage T4

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