The effect of ethynyl estradiol and desogestrel on proliferation and apoptosis hydatidiform mole trophoblast cell

Abstract

This study purpose to explore the effect of ethynyl estradiol and desogestrel on proliferation and apoptosis hydatidiform mole trophoblast cell. Hydatidiform mole tissue, trophoblast cells were isolated and culture at RPMI 20% FBS medium. Trophoblast cells divided into three groups observation. First group get ethynyl estradiol 10 nmol/mL, second group get desogestrel 100 nmol/mL, third group get DMSO 1%. Cells incubated and observe at 24, 48, 72, 96 hours. Cell cycle, apoptosis and β HCG was evaluated. The group of cells that get ethynyl estradiol in concentration 10 nmol/mL had cell proliferation index, amount cells and β HCG level higher than control after 72 hours observation. The group of cells that get desogestrel in concentration 100 nmol/mL have cell proliferation index, amount cells and β HCG level lower than control after 48 hours observation. There are no differences of apoptosis between the two group and control. Conclusion: Ethynyl estradiol will increase proliferation of hydatidiform mole trophoblast cell, while desogestrel will decrease proliferation of hydatidiform mole trophoblast cell. There is no effect of ethynyl estradiol and desogestrel on apoptosis of hydatidiform mole trophoblast cell.