The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound

Ajirni, Ajirni; Nazaruddin, Nazaruddin; Sutriana, Amalia; Masyitha, Dian; Isa, Muhammad Lokman

doi:10.21157/ijtvbr.v5i2.20484

Cited by 3 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fibroblasts actively move into the wound area to support the proliferating tissue. They also produce collagen, enabling the formation of granulation tissue that covers the wound [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Solanum tuberosum L. ointment on second-degree burns in mice

Silva-Correa,

Rosas-Cruz,

Calderón-Peña

et al. 2023

Vet World

View full text Add to dashboard Cite

Background and Aim: Potato (Solanum tuberosum L.) is mainly characterized by its antioxidant and healing properties. Therefore, this study aimed to evaluate the effects of an ointment based on S. tuberosum L. “papa tumbay” on burns induced in Balb/c mice (Mus musculus). Materials and Methods: The experimental animals were divided into four groups (n = 5/group) 48 h before second-degree burns were inducted. After epilating the loin areas of the mice and anesthetizing them with ketamine/xylazine (80 mg/kg/10 mg/kg) through intraperitoneal (i.p.) route, a round metal rod (0.7 cm in diameter) was placed on the depilated skin at a temperature of 100°C for 5 s. Group I was not given any treatment, Group II was treated with silver sulfadiazine (1%), and the other two groups (III and IV) were treated with the ointment formulated based on S. tuberosum L. “papa tumbay” at 1% and 2%, respectively. After performing the treatment for 21 days, the mice were euthanized using i.p. sodium pentobarbital (185 mg/kg) to obtain skin samples. The samples were preserved in 10% neutral-buffered formalin and subjected to histopathological analysis. Results: We found statistically significant differences in the histopathological sections between the groups (p < 0.05). The abundant collagen and fibroblasts observed in the direction of the dermis in Groups III and IV indicate that the phytoconstituents present in the potato might promote the healing of the second-degree burns until day 21 of treatment. Conclusion: Our findings showed that the ointments based on the ethanolic extracts of S. tuberosum L. “papa tumbay,” especially the 2% ointment, might accelerate the healing of second-degree burns induced in Balb/c mice. Keywords: burn healing, herbal medicine, histopathology, ointment, potato, skin, Solanum tuberosum L.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Solanum tuberosum L. ointment on second-degree burns in mice

Silva-Correa,

Rosas-Cruz,

Calderón-Peña

et al. 2023

Vet World

View full text Add to dashboard Cite

show abstract

“…Wounds are caused by sharp objects such as knives that damage the anatomy of the skin tissue which is characterized by the edges of the wound in the form of straight and regular lines. Wounds that are not treated can cause effects such as loss of tissue substance, bacterial contamination, and lead to complications such as infection 2 . Infection can occur when microorganisms enter the body and cause trauma or damage 3 .…”

Section: Introductionmentioning

confidence: 99%

Binahong leaf extract activity in the 8th day of wound healing infected with Staphylococcus aureus towards collagen tissue

Nuroini,

Kanti Ratnaningrum,

Mochamad Restinu Alya Soejoto

et al. 2021

J. Teknol. Laboratorium

View full text Add to dashboard Cite

Infection by Staphylococcus aureus, which contains alpha hemolysis and toxins that induce skin necrosis, can occur if incisions are left untreated. Infectious wound treatment that isn't dosed properly might have negative effects including bacterial resistance, hence plant-based solutions like binahong (Anredera cordifolia (Ten.) Steenis) leaf extract is needed. Alkaloids, saponins, and flavonoids found in binahong leaf act as anti-inflammatory, antiseptic, increase fibroblast cells, and increase collagen production throughout the healing process and scar tissue creation. Collagen production begins on day 3 of the proliferative stage and continues to increase until day 21. The goal of the study was to determine the thickness of collagen tissue using Masson's Trichrome staining on the 8th day of wound healing infected with S. aureus after administering binahong leaf extract. The research method used a completely randomized design with 4 research groups, normal control, negative control, treatment 25% (P1), and 50% (P2) concentrations of binahong leaf extract. S. aureus was cultured using BHI, MC, and BHIA media (Mc Farlan standard 0.5; 20L). Wounds were made on the back skin of the rat with a 2cm of length and 2mm of depth, then induced by S. aureus. The Binahong leaf extract was applied to the wound area in the morning and evening for 8th days. The paraffin method was used to prepare slides of the skin tissue, 10% NBF as fixation solution, and stained with Massonâ€™s Trichrome. The thickness was measured in 5 fields of view at 400x magnification with a score of 0-4. The results showed that the P2 group had the same average collagen thickness as the normal control group, which was 50% in each field of view (score 3). While the average thickness of the P1 group was 25% in each field of view (score 2). These results indicate that the administration of binahong leaf extract at 25% and 50% concentrations can stimulate the formation of collagen on S. aureus-infected wounds.

show abstract

Use of Medicinal Plants in the Process of Wound Healing: A Literature Review

Cedillo-Cortezano,

Martinez-Cuevas,

López

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

The literature on the use of medicinal plants in wound healing was comprehensively searched to obtain and assess the data. The data were procured via clinical studies that utilized medicinal plants and their compounds in vitro and in vivo for wound healing. This review collected data from electronic databases, including Google Scholar, PubMed, Science Direct, Web of Science, SciFinder, Thesis, and Scopus, using the search terms “natural products”, “wound healing”, and “natural compounds”, along with the keywords “plants”, “extracts”, and “phytochemicals”. Results from the last decade reveal a total of 62 families and 109 genera of medicinal plants, and their compounds have been studied experimentally both in vivo and in vitro and clinically found to effectively promote healing. This activity is related to the presence of secondary metabolites such as flavonoids, alkaloids, saponins, tannins, terpenoids, and phenolic compounds, which act at different stages through different mechanisms to exert anti-inflammatory, antimicrobial, and antioxidant effects, confirming that the use of medicinal plants could be an adequate alternative to current conventional practices for treating wounds.

show abstract

The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound

Cited by 3 publications

References 3 publications

Effects of Solanum tuberosum L. ointment on second-degree burns in mice

Effects of Solanum tuberosum L. ointment on second-degree burns in mice

Binahong leaf extract activity in the 8th day of wound healing infected with Staphylococcus aureus towards collagen tissue

Use of Medicinal Plants in the Process of Wound Healing: A Literature Review

Contact Info

Product

Resources

About